Biomedical subjects

K Dolezalová

Publications and source records attributed to K Dolezalová.

At least 19 recordsLinked to original sources

PubMed1989

[Monitoring serum theophylline levels in children. Use of saliva for prediction].

In 28 children suffering from asthma--20 boys and 8 girls aged 3-19 years, weighing 18-79 kg--theophylline concentrations in serum and saliva were assessed. For detection the spectrophotometric method of Schack and Waxler and the fluoroimmunoassay TDA AMES were used. Under conditions of non stimulated salivary secretion the pH values of the secretion varied as a rule between 6.0 and 7.5 which did not influence significantly the amount of theophylline passing from blood into saliva. The authors found a very close correlation between the theophylline concentrations after administration of Syntophylline in saliva and serum (r = 0.9169, p less than 0.001) and Spophylline (r = 0.9369, p less than 0.001). Comparison of serum theophylline concentrations calculated from the salivary values on the one hand and the same serum theophylline concentrations actually assessed revealed that their difference varied up to 1 microgram.ml-1.

Adolescent↗

PubMed1989

[Nonlinear theophylline kinetics in a boy on long-term theophylline therapy for bronchial asthma. Case report].

On the case of a 13-year-old boy the authors demonstrate the possible non-linear relationship between long-term administration of theophylline and its serum concentrations which implies the danger of induction of limital to toxic concentrations of the drug in the blood stream when the doses are increased as required.

Adolescent↗

PubMed1987

[Immunologic response in children treated with long-term bacterial autovaccination].

Explore the source record for details and available documents.

Bacterial Vaccines↗

PubMed1984

Phorbol myristate acetate-stimulated production of interleukin 2 by T-cell lymphoma and constitutive production by derived hybridomas.

To isolate a stable tumor cell line source of IL-2 (TCGF), 19 murine T-cell lines and their derivatives were screened for both constitutive and mitogen-stimulated IL-2 production. The cloned subline of a mouse thymic lymphoma EL-4 designated as EL-4TF could be stimulated with PMA to produce 80 U/ml of IL-2. A TK- EL-4TFR mutant line has been selected from the EL-4TF cell population by treatment with 5-BrdUrd. The EL-4-TFR cells were stimulated with PMA and fused with the cells of thymic lymphoma BW5147. The resulting BH3 hybrid cell population was repeatedly cloned and tested for constitutive IL-2 production: two of the BH3 hybridoma clones were found to produce IL-2 constitutively. The IL-2 of EL-4TF origin was found to support permanent in vitro growth of IL-2 dependent, tumor-specific T killer cell line CTLL when present in culture medium at a concentration of at least 0.1 U/ml. Since the EL-4TF-derived IL-2 preparations were contaminated with PMA, it was of interest whether PMA alone has a growth-promoting activity in CTLL cell cultures. Permanent cultivation of CTLL cells in an IL-2-free medium containing PMA was not possible. However, both mitogenic and co-mitogenic effects of PMA on CTLL cells were observed.

Animals↗

PubMed1983