Biomedical subjects
Publications and source records attributed to K E Balashov.
Immunological investigation was performed in patients with trigeminal neuralgies and painful myofascial temporomandibular joint dysfunction before and after treatment. The data suggest a connection between the state of humoral immunity and the peculiarities of pain syndrome in trigeminal neuralgia and clinically related prosopalgies that was most probably due to different mechanisms of realization of pain syndrome.
Natural antitumor resistance of cancer patients and healthy subjects was studied on the basis of whole blood natural cytotoxicity (WBNC) assay. A correlation between WBNC and mononuclear leukocyte cytotoxic activity in healthy donors was established (r = 0.912; p less than 0.001). WBNC levels in colonic, breast and gastric cancer patients were decreased as compared with healthy controls.
Adherent cells were released from human peripheral blood mononuclears and the remaining mononuclears were separated by the density gradient centrifugation on percoll. Five fractions obtained were tested for cytotoxicity against K-562 and HeLa tumour target cells. Cell morphology and reactivity to some monoclonal antibodies were also studied. Fractions 2 and 3 were enriched by large granular lymphocytes (LGL) and BMA-070 positive cells and showed increased cytotoxicity only to K-562 target cells, while fraction 1 contained 9.5% of monocytes, but no LGL, and showed increased cytotoxicity against both K-562 and HeLa target cells.
It is shown that the damage action of normal peritoneal macrophages of mice includes inhibition of the proliferating activity of the mastocytoma P-815 cells and their complete destruction accompanied by the breaks of their cytoplasmic membrane. Scanning electron microscopy was used to reveal two types of contacts between peritoneal macrophages and cells P-815; the loss of microvilli and the appearance of blebs on cell surface in the case of adsorption of P-815 cells on macrophages; the contact through long filopodia which are sent forth by macrophages in the case when contacting cells are at some distance from each other.
The results of immunologic investigation of patients with trigeminal neuralgia evidence that this condition involves impairments of local and central humoral immunity parameters: increased serum IgA and salivary secretory IgA. Such measurements may become a diagnostic test.
Studies of the major cell-mediated immunity parameters in patients with painful syndromes involving the face and neck have shown a reduction of the peripheral lymphocyte count.