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Biomedical subjects

K Emigholz

Publications and source records attributed to K Emigholz.

4 recordsLinked to original sources
PubMed1980

Protein patterns of early mouse embryos during development.

Using high resolution two-dimensional polyacrylamide gel electrophoresis, the major protein species (labeled in vitro with 35S-methionine) of mouse embryos were examined starting with the unfertilized egg up to the blastocyst stage (fourth day of development). The analysis was then continued using in vitro culture techniques up to the 10th equivalent gestation day. At all periods of development distinct protein changes could be seen. However, major alterations in the protein synthesis pattern were noted between the 2nd and 3rd day in vivo and around the 8th equivalent gestation day in vitro. A complete series of gels is presented such that proteins can be easily identified in terms of their molecular weights and isoelectric points.

Animals↗
PubMed1979

Increased amplification of pBR322 plasmid deoxyribonucleic acid in Escherichia coli K-12 strains RR1 and chi1776 grown in the presence of high concentrations of nucleoside.

When pBR322 plasmid-harboring Escherichia coli strains RR1 or chi1776 were grown in the presence of 1 mg of uridine or cytidine per ml and later treated with chloramphenicol, as much as three times more plasmid deoxyribonucleic acid was recovered than would normally be obtained by routine plasmid amplification procedures.

Culture Media↗