Biomedical subjects

K F GIVAN

Publications and source records attributed to K F GIVAN.

3 recordsLinked to original sources

PubMed1965

INCIDENCE OF RUBELLA ANTIBODIES IN FEMALE SUBJECTS.

Sera from females aged 1 to 40 years were assayed for rubella virus antibodies. Results showed that by age 14 years, 60% had antibodies and that by 19 years, 70% were positive. This figure rose to 80% by 24 years of age and remained unchanged in older age groups.A comparison of the incidence of high and low levels of antibodies in each age group revealed that antibody levels fell between ages 20 and 40 years. Only 20% of individuals in the latter group had a high antibody level compared to 80% in the former.These results are discussed as they relate to the problems of reinfection and possible vaccination procedures.

Adolescent↗

PubMed1963

A plaque method for rubella virus assay.

A method has been described in which suitable dilutions of rubella virus will induce the formation, in a monolayer of green monkey kidney cells, of islets of infected cells which were protected from the effects of Echo 11 challenge virus. The number of islets or "negative" plaques was proportional to the dilution of rubella virus inoculated on to the monolayer. Using this method, it was observed that bentonite adsorption increased the plaque assay values of rubella virus pools. This suggested that rubella virus interference may be mediated by an interferon-like principle.

Animals↗

PubMed1963

Association of virus with cases of rubella studied in Toronto: propagation of the agent and transmission to monkeys.

Using an interference test with indicator virus Echo 11, a virus has been isolated in nine of 18 specimens from cases of typical rubella. The virus will interfere with the development of cytopathology in green monkey kidney cells with viruses Echo 11, Coxsackie B1 and B4, Poliovirus I and III (Sabin strains) and simian virus SV4. In four of five paired sera this virus was neutralized by convalescent but not by the acute phase serum, tested by interference inhibition. No cytopathology was observed in unstained cultures or in sequential cultures stained with acridine orange or fluorescent antibody. The virus was destroyed by exposure to 56 degrees C. for 30 minutes and 15% ether at 4 degrees C. for 24 hours, but survived with some reduction in titre at 4 degrees C. for 24 hours. Green monkeys infected by this virus developed a macular rash, lymphadenopathy and modest rise in white blood cell count.

Animals↗