Development of effective strategies to support breastfeeding.
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Adult↗
Biomedical subjects
K Farley
Publications and source records attributed to K Farley.
The COPD critical pathway: a case study in progress.
Patients with chronic obstructive pulmonary disease (COPD) consume many health care resources and require complex coordination of care among multiple caregivers. In this report, we share our experiences at Fletcher Allen Health Care, Burlington, Vermont, in developing and implementing a critical pathway for these patients. The COPD pathway has resulted in measurable improvements in the quality of care and has provided us with lessons that will enhance our use of critical pathway methods.
Clinical Protocols↗
Development of solid-phase enzyme-linked immunosorbent assays for the determination of epidermal growth factor receptor and pp60c-src tyrosine protein kinase activity.
Solid-phase ELISAs for the determination of EGF receptor (EGF-R) and pp60c-src tyrosine protein kinase activity are described. The methods were developed and optimized using purified recombinant EGF-R intracellular domain (ICD) and pp60c-src tyrosine protein kinases. A standardized assay that utilizes poly (GluNa-Tyr)4:1 as substrate and a monoclonal antiphosphotyrosine antibody for detection is described. Assay conditions for both enzymes were optimized with respect to substrate and ELISA plate-coating condition, divalent metal ion preferences, enzyme concentration, apparent kinetic constants for ATP, and reaction linearity. Following standardization, a number of reference tyrosine protein kinase inhibitors were tested in the ELISAs and compared to results obtained using solution-phase radioactive tyrosine protein kinase assays, which are based on the transfer of 32P from [gamma-32P]ATP to synthetic substrate. To enable a comprehensive comparison, IC50 values obtained in the ELISA were compared with values obtained in radioactive assays using both the holo-EGF-R and EGF-R ICD kinases. No substantial qualitative differences between these assays were seen. For many routine tyrosine protein kinase assays, semiquantitative or qualitative measurement of TPK activity is adequate. For such purposes, the ELISAs would be an attractive alternative to radioactive assays.
Enzyme-Linked Immunosorbent Assay↗
A research-based risk assessment tool as the cornerstone of a pressure ulcer prevention program.
By highlighting theories of knowledge utilization, we consider some of the factors and issues in planning research utilization projects. By our use of a research utilization model in developing a research-based pressure ulcer prevention program, we help to bridge the gap between research and practice.
Clinical Nursing Research↗
A chemotherapy standard order form: preventing errors.
PURPOSE/OBJECTIVES: To describe the development and implementation of one approach to standardize and document chemotherapy orders for patients in the acute-care setting. DATA SOURCES: Multidisciplinary team, Oncology Nursing Society standards of care, clinical experience, and published literature. DATA SYNTHESIS: To avoid errors in ordering, dispensing, and administering chemotherapy that may have the potential for serious adverse patient outcomes, a standardized order sheet was developed to consistently document information regarding chemotherapy ordering and administration throughout the hospital. Each component of the form is essential in promoting safety and efficiency in the chemotherapy ordering and administration process. CONCLUSION: A standardized approach dramatically improved ordering, dispensing, and administering chemotherapy. Multidisciplinary verification and documentation of dose and schedule helps reduce chemotherapy-related errors. IMPLICATIONS FOR NURSING PRACTICE: The chemotherapy order sheet saves time and is comprehensive and consistent. Use of the order sheet also has increased interdisciplinary collaboration and assisted all members of the team in documenting required information.
Antineoplastic Agents↗