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Biomedical subjects

K Funatsu

Publications and source records attributed to K Funatsu.

At least 37 records · Page 2Linked to original sources
PubMed1994

A case of a primary hepatic tumor causing segmental changes on imaging and its relation to Zahn's infarct.

A 69-year-old female was admitted to our hospital for further examination of an intrahepatic mass which had been found while undergoing a complete physical examination. The mass measured 4 cm in size and was located in the medial segment (S4) of the liver. On computed tomography (CT), S4 was observed to be 'atrophied' and was well enhanced segmentally. A celiac angiogram showed segmental staining, and a transarterial portogram demonstrated portal stoppage of S4 from the left branch. However, no segmental intensity difference was seen on magnetic resonance imaging (MRI). An aspiration biopsy showed adenocarcinoma and thus an operation was performed under a tentative diagnosis of intrahepatic cholangiocarcinoma. The postoperative diagnosis of the tumor was combined hepatocellular and cholangiocellular carcinoma. However, no histological abnormality was seen in S4, contrary to the expectation of Zahn's infarct. In this study, we discuss the mechanism and imaging findings of Zahn's infarct, the possible reasons as to why no pathological change was seen in S4, as well as stress the rarity of reports on Zahn's infarct in cases of portal thrombus due to hepatocellular carcinoma.

Aged↗
PubMed1992

Adenine nucleotide content of thymoma-derived lymphocytes.

Purine and pyrimidine metabolites are essential substances for cells. We have measured the adenine nucleotide (AN) contents of thymocytes from 15 human thymomas, 11 adjacent non-neoplastic thymuses and 3 children's thymuses. There was no significant difference in AN content of thymocytes between thymoma and children's thymus. But, AN content of adjacent non-neoplastic thymuses was significantly lower than that of thymoma or children's thymus. In mixed type thymoma, ATP content in thymocytes was significantly higher than that in lymphocytic type thymoma. These data indicate that thymocytes in thymoma may show further T cell maturation probably associated with the functional microenvironment of the neoplastic epithelial cells just like the children's thymus.

Adenine Nucleotides↗
PubMed1991

High density culture of anchorage-dependent animal cells by polyurethane foam packed-bed culture systems.

Monkey kidney cells (Vero) and Chinese hamster ovary cells (CHO-K1) attached to the internal surface of polyurethane foam (PUF) and grew to a high cell density (1.1 x 10(8) cells/cm3 PUF and 4.2 x 10(7) cells/cm3 PUF, respectively) in a PUF-plates packed-bed culture system. This density of Vero cells was twice that obtained previously with a PUF-particles packed-bed culture system. A maximum cell density of 6.7 x 10(7) cells/cm3 culture vessel volume was obtained in a PUF-disc packed-bed culture of Vero cells. From the cell density of CHO-K1, growing in a monolayer on the surface of PUF and a petri dish, per bulk volume of PUF, we estimated that a surface area to volume ratio of PUF plates effective for cell growth was about 109 cm2/cm3.

Animals↗
PubMed1991

High albumin production by multicellular spheroids of adult rat hepatocytes formed in the pores of polyurethane foam.

Adult rat hepatocytes formed spherical multicellular aggregates (spheroids) when they were cultured in the pores of polyurethane foam (PUF). The diameter of the spheroids was within the range 100-200 microns. These spheroids partly attached and immobilized in the PUF pores for at least 2 weeks. The albumin production rate by the spheroids increased up to 17.0 micrograms/10(6) nuclei per day during the first 6 days and maintained at a high level for 2 weeks. In contrast, the albumin production rate by the monolayer markedly decreased after 3 days. The spheroid culture using PUF seems to be a convenient and simple method for maintaining some differentiated functions of hepatocytes and for making a bioreactor using the function of spheroids.

Albumins↗
PubMed1990

Anchorage-dependent mammalian cell culture using polyurethane foam as a new substratum for cell attachment.

Anchorage-dependent mammalian cells were cultivated at high cell density in a novel culture system using polyurethane foam (PUF) as a substratum for cell attachment. PUF has a macroporous structure giving a high surface area to volume ratio. Monkey kidney cells (Vero) and Chinese hamster ovary cells (CHO-K1) attached to the internal surface of PUF and grew to a high cell density (1.04 X 10(8) cells/cm3 PUF and 3.5 X 10(7) cells/cm3 PUF, respectively) in PUF stationary cultures. In addition, we have designed a PUF-particle packed-bed culture system for high density mass cell culture. A maximum cell density of 2.4 X 10(7) cells/cm3 culture vessel volume was obtained in a packed-bed culture of Vero cells.

Animals↗
PubMed1990

Increased gallium-67 citrate uptake in the breast of a patient with gynecomastia.

Although the accumulation of Ga-67 citrate in gynecomastia has been described, no researchers have reported that Ga-67 citrate accumulates in a "doughnut" pattern. Basically, gynecomastia is due to an increase of estrogens. Since estrogens accelerate the proliferation of lactiferous ducts in the center of breast, Ga-67 citrate may accumulate in it. On the hand, prolactin increases the proliferation of the glandular lobules and the acinus. When the serum prolactin increases after child birth or renal insufficiency, the image from the accumulation of Ga-67 citrate in the breast may have a "doughnut" pattern. A patient with gynecomastia had high levels estrogens because he was being treated with an estrogen medication. He was referred for liver cirrhosis. The results of the present report indicate that the "doughnut" pattern occurs in the margin of the hyperplasic glandular lobule.

Citrates↗
PubMed1990

Changes in thymocytes due to aging--a study of the effects of thymic hormone on adenine nucleotides and thymocyte DNA.

The number of lymphocytes (especially T-lymphocytes) in peripheral blood decreases with aging. The decline is most evident in the thymus. In this study, total AN in the thymocytes of aged mice clearly decreases from that of young mice. The quantity of thymic hormones secreted by thymic cells decreases with the organism's thymus involution with age. After demonstrating the decrease in total AN (primarily ATP) levels within the thymocytes of aged mice, we attempted to reattain the levels of younger thymocytes by the administration of various thymic hormones. Examination of DNA in the thymocytes of 4 and 58-week-old mice, using flowcytometry, showed no differences in content. However, it is significant that aged mouse thymocytes's energy level can be returned to those of young mice with the application of Th-5.

Adenine Nucleotides↗
PubMed1990

[Increased gallium-67 citrate uptake in the breast of a patient with gynecomastia].

The patient was a 60-year-old male. He had undergone orchiectomy 5 years earlier because of prostatic cancer. During this interval, he has taken the estrogen. Scintigraphy revealed on accumulation of Ga-67 citrate in the primary/metastatic foci. Of interest, the image of the accumulation of in the breast demonstrated a "doughnut" pattern.

Breast↗
PubMed1989

Microcirculatory disturbances of oral mucosa and periodontal disease in patients with liver cirrhosis.

This study showed: (1) The bone score in cirrhotic patients was significantly lower than that in the control group. (2) In liver cirrhosis, capillaries were decreased in number and they were dilated compared to that in the control. In particular, the capillaries in cirrhotic patients with ascites or oesophageal varices were dilated compared to those without. (3) Blood flow of the mucogingival junction was significantly decreased in patients with liver cirrhosis in comparison with the control group. These results suggest that microcirculatory disturbances of oral mucosa are important in the progress of periodontal disease in patients with liver cirrhosis.

Female↗
PubMed1986

5-Hydroxytryptamine and (+)-lysergic acid diethylamide displace [3H]thyrotropin-releasing hormone at its binding sites in the rat limbic forebrain.

Binding of [3H]thyrotropin-releasing hormone (TRH) to its receptors in the rat limbic forebrain was partially displaced by 5-hydroxytryptamine (5-HT, ligand for 5-HT1 receptors) and (+)-lysergic acid diethylamide ((+)-LSD, ligand for 5-HT1 and 5-HT2 receptors) at nanomolar concentrations. Spiperone (ligand for 5-HT2 receptors) displaced [3H]TRH in a dose-dependent manner at micromolar concentrations. These results suggest that some TRH receptors are related to 5-HT1 receptors, probably adjoining them on the membrane. This type of TRH receptor is shown to be among the high-affinity receptors which we reported previously. The significance of the receptor-coexistence is such that TRH facilitates serotonergic transmission by increasing the density of 5-HT1 receptors. This finding seems to support a pharmacological observation of other investigators that TRH potentiates 5-HT-induced hyperactivity in mice, probably by affecting postsynaptic 5-HT receptors.

Animals↗
PubMed1985

[Hyperbaric oxygenation treatment of the after-effects of carbon monoxide poisoning].

Following a symptom-free interval of 2 weeks after acute carbon monoxide poisoning a 38-year-old previously healthy man gradually developed impairment of drive and movement, disturbed memory, disorientation, mutism and abnormal gait. Finally, an apallic syndrome developed. After a total of 40 periods of hyperbaric oxygen administration the patient recovered completely and resumed his work. The EEG improved during the treatment more rapidly than the clinical picture. The electropolysomnograph changed in parallel to the clinical picture. Computed tomography on admission to hospital demonstrated a zone of lower density in the region of the right globus pallidus. This finding was no longer demonstrable after the patient had fully recovered.

Adult↗
PubMed1985

Various types of thyrotropin-releasing hormone receptors in discrete brain regions and the pituitary of the rat.

Receptors for thyrotropin-releasing hormone (TRH) in the rat brain and the pituitary are heterogenous. The receptors were classified into four types according to the dissociation constant (KD). High-affinity receptors (KD less than 3 nM) are present in the pituitary, hypothalamus, amygdala, and limbic forebrain which contains the nucleus accumbens and the septum. Intermediate-affinity receptors (KD, 5-16 nM) are evidently present in the frontal cortex, hippocampus, striatum, thalamus, and the brainstem, but may also be present in other regions. Low-affinity TRH receptors (KD, 50-80 nM) are seen in the limbic forebrain, amygdala, and the hypothalamus. Very-low-affinity receptors (KD, 215 nM) exist in the pituitary. Experiments using DN-1417 (gamma-butyrolactone-gamma-carbonyl-histidyl-prolinamide citrate), a synthetic TRH analogue with a more potent central activity, indicated the presence of TRH receptors having a high affinity to DN-1417 at least in the limbic forebrain but not in the pituitary. This type of receptor is not labeled by [3H](3-methyl-histidine2)-TRH. Density of the TRH receptor is the highest in the pituitary and next highest in the amygdala.

Amygdala↗