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K G Bergner

Publications and source records attributed to K G Bergner.

6 recordsLinked to original sources
PubMed1986

[Proteins of bee honey. VI. Isoelectric focusing of amylase in various kinds of honey].

The isolated honey amylase was characterized by preparative and analytical isoelectric focusing. An interesting aspect of the preparative isoelectric focusing was the reduction of iodine by Ampholines which were used for building up a pH-gradient, giving a false increase in apparent amylase activity as determined by the iodine/starch reaction. Dextran gels were not suited for the matrix, because interactions with the enzyme disturb the focusing. From the analytical isoelectric focusing of isolated honey amylase in ultrathin layer Polyacrylamide gels 10 protein bands resulted. A newly developed zymogram technique was positive for amylase activity in all 10 protein bands.

Amylases↗
PubMed1977

[The proteins of honey. III. Separation of honey amylase by hydrophobic partition chromatography (author's transl)].

Previous experiments to separate the amylase and "invertase" of honey by chromatography on sephadex-gels were unsuccesful. It was shown that honey-amylase, -like amylases form other sources -- has hydrophobic properties. Therefore it was possible to separate amylase-activity from other activities by means of hydrophobic affinity chromatography on phenyl-butylamine-Sepharose 4B.

Amylases↗
PubMed1975

[Proteins in honey. II. Gel-chromatography, enzymatic acitivity and origin of honey-proteins (author's transl)].

The honey proteins were separated into five distinct peaks by freezing and thawing and subsequent chromatography on Sephadex G-200. The enzymatic activities were always located in the same peaks and clearly separated. The elution curves of the individual honeys were very similar. No significant differences were observed neither among floral honeys not between floral and honeydew honeys. The determination of the elution coefficients, however, showed that three of the five peaks originate in the bee the other two in the plant.

Chromatography, Gel↗
PubMed1973

[Melting salts].

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Cheese↗