Biomedical subjects

K G Karagezian

Publications and source records attributed to K G Karagezian.

At least 19 recordsLinked to original sources

PubMed2006

[Study of volatile fatty acids in the blood of patients with periodic disease].

Microecological "failures" are an important pathogenetic factor of different diseases and, in the authors' opinion, periodic disease (PD) is one of them. PD is a recessive disease characterized by fever attacks and neutrophil-mediated serous inflammation. A genetic factor has been established to be responsible for half the cases of PD, the influence of non-hereditary factors, particularly a role of the host automicroflora in the genesis of an inflammatory process, has been little studied. The authors' early studies indicate that there are changes in the qualitative and quantitative composition of microbial molecules in the blood of patients with PD. The anaerobic bacterial metabolites that are volatile fatty acids (VFAs) represent biologically active substances that affect the growth of the microflora, on the one hand, and the host's immunological responsiveness, on the other. Out of VFAs, only is acetic acid detectable in small quantities in the blood of healthy individuals. The other VFAs, namely propionic, valeric, butyric, and caproic acids and their isomers, are absent. Gas chromatography was used for qualiitative and quantitative determination of the metabolites of anaerobic microorganisms in the blood of patients with PD (n = 13) during an attack and remission and in that of healthy volunteers (Armenians) (n = 5) of a control group from one Yerevan region. The blood samples from all the patients with PD displayed a significantly higher concentration of caproic acid while the latter was absent in the blood of the controls. This finding suggests that there is a specific shift in the structure of the microbiocenosis in patients with PD. It is conceivable that caproic acid plays a certain role in the pathogenesis of the disease under study. Further studies will deal with the association of some microbial molecules with the manifestation of an attack of PD, which may provide the key to the goal-oriented regulation of detected homeostatic disorders and to the management of the frequency of its attacks.

Armenia↗

PubMed2004

[Nuclei and nucleolar organizing regions in chromosomes of lymphocytes on different stages of periodic disease].

By scanning cytomorphometry a cytological study was first performed on the behavior of nuclei and nucleolar organizing regions (NOR) in chromosomes of peripheral blood lymphocytes of healthy men and of patients with periodic disease (familial Mediterranean fever, FMF) on different stages of development, including its complication with amyloidosis. The volume and total surface of nuclei, the sum total volume and sum total surface of NOR, the mean number of NOR for one nucleus and distribution of nuclei according to NOR number were measured. It is shown that the parameters of nuclei and NOR for patients with FMF on all stages clearly and trustworthy differ from those for healthy men. They are sufficiently informative, can be successfully used in clinical practice and even serve as an early diagnostic test for amyloidosis complication.

Amyloidosis↗

PubMed2002

[The spectrum and level of low-weight molecular compounds of microbial origin in familial Mediterranean fever].

Familial Mediterranean fever (FMF) is a disease whose etiopathogenesis is not clarified yet. The infectious theory of FMF has not been confirmed either. Nevertheless, the involvement of microbes in the trigger of an inflammatory process cannot be excluded since today's well-known pathogenetic processes in FMF are to be directly related to the key cells of an inflammatory response. According to the existing concept, homeostasis of small molecules originating from microbes (SMOM) in healthy individuals is achieved due to adequate immune system function with the preserved biocenosis of a macroorganism whose disturbance with resultant immune shifts triggers a cascade of inflammatory reactions in the body. An attempt was taken to reveal the participation of microorganisms at the onset of an inflammation in case of FMF by using chromatographic mass spectrometry to detect chemical components of microorganisms and their vital activity products. The method allows one to screen a large number of microbial markers in a clinical sample. Pronounced impairments in the homeostasis of non-protein SMOM were found in the blood of examined patients with FMF (n = 16). There was a uniformity of deviations from the normal values in all the examinees in the episodes and episode-free periods. These qualitative and quantitative deviations basically differ from the direction of changes with other diseases (n 59) or healthy individuals (n = 18). All significant deviations affect non-traditional participants of an inflammatory process in the host. The similar microecological breakages in the human body and their consequences have not been earlier detected and investigated. The findings show it necessary to continue studies to receive an answer to the question as to whether the detected homeostatic features of SMOM in patients with FMF are primary or what is their role in the etiopathogenesis.

Adolescent↗

PubMed2000

[Immune disorders in periodic disease].

The examination of 404 patients with periodic disease (301 with uncomplicated form and 104 with amyloidosis complication) has detected decreased functional activity of T-lymphocytes and their suppressor subpopulation, their subnormal quantity. There was a rise in T-helper/T-suppressor index, level of B- and 0-lymphocytes, sensitivity to the renal antigen. Colchicin therapy stopped the attacks and stimulated T-suppressor activity. Combined treatment with tactivin is proposed.

Adolescent↗

PubMed2000

[Immunological indices in development of periodic disease].

AIM: To specify immune changes in periodical disease (PD) in different periods of PD development as well as peculiarities of their changes in colchicin therapy. MATERIAL AND METHODS: Clinicoimmunological investigations including blast transformation of lymphocytes with lymphocytic mitogen and renal antigen, leukocyte migration inhibition with renal antigen, spontaneous and complementary rossette formation were made in 828 PD patients and 43 donors. RESULTS: Decreased functional activity and quantity of T-lymphocytes, their suppressory subpopulation was found in PD. The indices did not correlate with the disease variants and gender but with age and PD complication. In amyloidosis immunological indices deteriorate with positive trend after colchicin treatment. CONCLUSION: PD is characterized by strain in immunological process.

Adolescent↗

PubMed1999