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Biomedical subjects

K Gebhardt

Publications and source records attributed to K Gebhardt.

16 recordsLinked to original sources
PubMed1992

Escherichia coli K-12 and B contain functional bacteriophage P2 ogr genes.

The bacteriophage P2 ogr gene encodes an essential 72-amino-acid protein which acts as a positive regulator of P2 late transcription. A P2 ogr deletion phage, which depends on the supply of Ogr protein in trans for lytic growth on Escherichia coli C, has previously been constructed. E. coli B and K-12 were found to support the growth of the ogr-defective P2 phage because of the presence of functional ogr genes located in cryptic P2-like prophages in these strains. The cryptic ogr genes were cloned and sequenced. Compared with the P2 wild-type ogr gene, the ogr genes in the B and K-12 strains are conserved, containing mostly silent base substitutions. One of the base substitutions in the K-12 ogr gene results in replacement of an alanine with valine at position 57 in the Ogr protein but does not seem to affect the function of Ogr as a transcriptional activator. The cryptic ogr genes are constitutively transcribed, apparently at a higher level than the wild-type ogr gene in a P2 lysogen.

Amino Acid Sequence↗
PubMed1988

[Development and course of functional urinary retention in paraplegic patients].

The urographic follow-up series of 453 patients of a paraplegic rehabilitation center are checked to obstructive uropathies. In 33 patients (7.3%) mostly asymptomatic obstructions were found. The onset of the urinary transport disorder is in 71% of the patients in the first year after the accident. Therefore, roentgenological and urodynamical investigations are necessary in the first years after the paralysis. The urological follow-up must be done for life as secondary morphological changes of the urinary tract are the cause of late obstructions. The prognosis of urinary transport disorders of the upper urinary tract is favourable in the case of timely and purposeful treatment. The development of a high-pressure system of the urinary bladder is the most important causal factor for qualified urodynamical diagnostic measures. Therefore, the therapeutic influence on the development of this high-pressure system is important in the prophylaxis and therapy of urinary obstruction.

Adult↗
PubMed1988

[The status of intermittent self catheterization in the long-term management of paraplegic patients].

The search for an optimal therapy of the disturbance of bladder function which required much extensive diagnostical measures may be long-term and much patience of both sides is necessary. A number of conservative measures is possible, but the most expensive therapy must not be the best treatment. We would be remember to a simple and safe method, but to its successful long-term application a good functioning care system is mandatory.

Adult↗
PubMed1986

[Indications and results of transurethral 12 o'clock sphincterotomy in the therapy of neurogenic disorders of bladder emptying in transverse paralysis].

In 23 male patients with tetra- and paraplegia of a rehabilitation clinic for patients paralysed by a transverse lesion of the cord the transurethral 12-o'clock-sphincterotomy of the external vesical sphincter was performed on account of the neuromuscular dysfunction of the urinary bladder. In 78% of the patients the comparison of preoperative and postoperative clinical and urodynamic findings yields a positive result, i.e. decrease of the residual urine, reduction of the micturition pressure, regression of urinary stasis and reflux. The method is suited to influence favourably the life of these patients.

Humans↗
PubMed1982

[Drug therapy of neurogenic bladder with the alpha-receptor blocker phenoxybenzamine].

A report is given on the results of therapy with the alpha-adrenolytic phenoxybenzamine in 36 patients in a rehabilitation centre for paraplegics. The sole indication for using the preparation was neurogenously disturbed micturition with large quantities of residual urine. The best therapeutic results--i.e. reduction of residual urine to 60 ml or less--were achieved in patients with lesions of the lower motoric neuron (15 patients) and the upper motoric neuron in the region L2-S2 (1 patient). The results in cases of lesion in the region Th10-L2 (3 patients) were so bad that the application of the preparation cannot be recommended in such cases. In cases of paralysis of the thoracic cord and the cervical part of the medulla (14 patients) 50% of the results were good and 35% were bad.

Adolescent↗
PubMed1981

[Hyperoncotic hemodilution in the ewe asa model of therapy in EPH gestosis (author's transl)].

Hyperoncotic hemodilution is proposed as a new therapeutic procedure in EPH-gestosis for improvement of uterine blood flow. By infusion of human albumin solutions in acute experiments on pregnant Merino sheep the effects of altered maternofetal colloid osmotic pressure gradients were investigated. The proposed hemodilution treatment improved uterine blood flow without a theoretically apprehended and undesired fetal hemoconcentration. An absolute indication for hypervolemic EPH-gestosis is suggested in accordance with modern knowledge of the pathophysiology of microcirculation. Isovolemic hemodilution is proposed to be of potential value in cases of EPH-gestosis with normovolemia, hypertension, intrauterine fetal growth retardation or other symptoms of placental insufficiency, at least as a subject to further studies.

Amniotic Fluid↗
PubMed1977

[The effect of nitrates on the function of intact and ischemic myocardium (author's transl)].

The effects of Sodium Nitroprusside (NPN), of Isosorbidedinitrate (ISDN), and of a combined therapy with NPN and the intraaortic balloon pump (IABP) on left ventricular function and on local contraction patterns of normal and ischemic myocardial regions were studied. In acute experiments on 16 dogs the anterior descending branch of the left coronary artery was stenosed to produce a hypokinesia or dyskinesia of the anterior wall of the left ventricle. Regional myocardial function was assessed using the ultrasound transit time method. When the LV filling pressure was initially norman (LVedP 5.7 +/- 0.6mmHg) nitrate infusion (120 microgram/minn NPN: 800 microgram/min ISDN) resulted in an aggravation of the ischemic dysfunction. Conversely, with high initial filling pressure (LVedP 15.4 +/- 1.3MMHg) regional myocardial function in the ischemic area returned to normal under therapy. Additional application of the IABP further improved regional myocardial mechanics. It is concluded, that reduction of coronary perfusion pressure due to nitrate therapy becomes critical when coronary reserve is depleted. Compensation is however possible if ventricular wall stress can be reduced and thus the oxygen consumption and the extravascular component of coronary resistance are decreased. Only when the initial filling pressure is high these conditions are met.

Animals↗
PubMedDate not supplied

Pressure points.

Explore the source record for details and available documents.

Humans↗
PubMedDate not supplied

Heart preservation by means of a complex artificial perfusion fluid.

Heart storage for different time intervals of up to 72 hours was carried out using a complex perfusion fluid elaborated according to a personal new theoretical concept. The recovery of the hearts stored by means of this fluid was practically integral as shown by the measurements and recordings of several cardiac functional parameters within a period of 5-7 hours of ex vivo functional testing subsequent to the storage. This fluid was also found to allow repeated heart storages for 24 hours alternating with efficient ex vivo functional testing for periods of 5-7 hours. Such performances in heart preservation were not yet reported elsewhere. This fluid was thought to allow an efficient ex vivo heart conditioning before transplantation by immunological or pharmacological treatments throughout repeated short-term or long-term alternating storages and recoveries.

Amino Acids↗