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Biomedical subjects

K Gilmore

Publications and source records attributed to K Gilmore.

10 recordsLinked to original sources
PubMed2000

Expression of the cyclic AMP-dependent transcription factors, CREB, CREM and ATF2, in the human myometrium during pregnancy and labour.

Elevated concentrations of cyclic AMP (cAMP) in the human myometrium may promote uterine quiescence during pregnancy by protein kinase A (PKA)-mediated phosphorylation and subsequent inactivation of myosin light-chain kinase, as well as by the phosphorylation and activation of cAMP-dependent transcription factors. In this context, we show that the altered expression of cAMP response-element binding protein (CREB), cAMP response-element modulator protein (CREM) and activating transcription factor 2 (ATF2) are implicated in the maintenance of myometrial quiescence during fetal maturation and the switch to uterine activation at term. Using electrophoretic mobility shift and super shift assays, as well as immunoblotting of paired myometrial tissue samples from non-pregnant, pregnant non-labouring and spontaneous labouring women, we defined the patterns of expression of various isoforms of these proteins in the human uterus. Here, we report spatio-temporal changes in the expression of a 43 kDa form of CREB, a 28 kDa CREM-like protein, and a novel 28 kDa ATF2-like protein which are differentially expressed, depending on the gestational state of the uterus. Changes in the pattern of expression of these potent transcription factors may have an important role in the control of uterine activity throughout pregnancy.

Activating Transcription Factor 2↗
PubMed2000

A psychoanalytic perspective on attention-deficit/hyperactivity disorder.

Psychoanalysts have tended to view the diagnosis of AD/HD either with skepticism or as a contraindication to analytic treatment. The author reviews the history of this puzzling diagnostic entity, which is estimated to account for up to fifty percent of child referrals, and suggests that a psychoanalytic perspective on the underlying disturbance in ego functioning helps to clarify the nature of the symptom picture. Whatever the etiology, which may be compound, she suggests that psychoanalytic treatment, most often in conjunction with psychopharmacological therapy, can address the core disturbance in ego integration that creates the familiar triad of inattention, impulsivity, and hyperactivity.

Attention Deficit Disorder with Hyperactivity↗
PubMed1999

The use of chloromethyl-X-rosamine (Mitotracker red) to measure loss of mitochondrial membrane potential in apoptotic cells is incompatible with cell fixation.

BACKGROUND: A recent report by Macho et al. (Cytometry 25: 333-340, 1996) described the use of chloromethyl-X-rosamine (CMX-Ros) as a fixable probe for detection of loss of mitochondrial membrane potential (psi(mit)), an early event in many models of apoptosis. However, this previous report lacked a description of any direct comparisons between pre- and post-fixation analyses of normal and apoptotic cells stained with CMX-Ros. METHODS: Using a variety of cell types, we investigated the effect of paraformaldehyde fixation on cellular retention of CMX-Ros and the implications of this for the subsequent analysis of changes in psi(mit) in cells undergoing apoptosis. RESULTS: We found that following fixation, the resolution between normal cells with polarized mitochondria and apoptotic cells with depolarized mitochondria is reduced to the extent that accurate discrimination between the cell types is no longer possible. CONCLUSIONS: Overall, our results are consistent with CMX-Ros being a valid probe for psi(mit) in intact cells but only when the cells are stained and analyzed immediately. Thus, our results suggest that the proposed applications for CMX-Ros in multiple parameter analysis of fixed cells are inappropriate and will lead to spurious results.

Apoptosis↗
PubMed1998

Cloacal anxiety in female development.

Contemporary psychoanalytic theory about feminine genital anxieties has shifted our focus away from some essential developmental realities of the little girl prior to the oedipal phase. Anal phase experience has recently been reemphasized as contributing significantly to the girl's developing body image, her mastery modes, and her psychological experience of gender. This paper proposes that associated with this phase is a specific anxiety described as the girl's fear that her genital is dirty, messy, and repellent to the love object, and that her sexuality is out of control and dangerously explosive. This is an important anxiety for the girl, a specific feminine version of anxiety around loss of love and approval. It derives from her bodily experience and is typically incorporated into the feminine genital anxieties of the oedipal period.

Adult↗
PubMed1995

Gender identity disorder in a girl: insights from adoption.

Gender identity disorder in girls is reported far less frequently than in boys. The analysis of a six-year-old adopted girl with gender identity disorder is presented to show the importance of the mother-infant relationship and of parental, especially maternal, attitudes toward gender in the normal development of gender identity and gender role definition. This child's unusual history included a late adoption, with clear evidence of separation trauma, and the ongoing influence of maternal infertility. Her symptoms of gender identity disorder appeared before her second birthday and were well established by three years of age. The analysis, from age six to thirteen, achieved a better adaptation to her gender. Her masculine strivings gradually became incorporated into an essentially feminine orientation.

Adolescent↗
PubMed1994

Androgen and oestrogen responsiveness of stromal cells derived from the human hyperplastic prostate: oestrogen regulation of the androgen receptor.

Stromal cells derived from collagenase-digested benign hyperplastic adult prostates were isolated and grown in culture. Androgen and oestrogen receptor status were determined and growth in response to mibolerone (a synthetic androgen) and oestradiol-17 beta was measured. In addition, the ability of oestrogens to regulate the androgen receptor in stromal cells was investigated. [3H]Thymidine incorporation into DNA was stimulated by mibolerone in primary and secondary cultures, but sensitivity was lost with subsequent passages. Androgen stimulation of [3H]thymidine incorporation was consistently inhibited by the anti-androgen cyproterone acetate. Oestradiol-17 beta also stimulated [3H]thymidine incorporation into DNA, and this effect was inhibited by the anti-oestrogen tamoxifen. Sensitivity to oestradiol was lost with subsequent passages. A combination of mibolerone and oestradiol was not synergistic in increasing [3H]thymidine incorporation into DNA, but maximal stimulation occurred at 100-fold lower concentrations of mibolerone and oestradiol when the two hormones were applied in combination. Specific high-affinity [3H]mibolerone- and [3H]oestradiol-binding sites were demonstrated by radioligand binding in intact cells. The affinity for oestradiol binding to its receptor exceeded that quantified for mibolerone binding to the androgen receptor, whilst the number of oestradiol-binding sites was approximately tenfold less than that quantified for mibolerone. Treatment with oestradiol down-regulated the number of [3H]mibolerone binding sites 1.7-fold (P < 0.005) as early as day 2 after oestradiol treatment. In conclusion, we successfully cultured stromal cells derived from hyperplastic prostates which retained sensitivity to androgen and oestrogen.(ABSTRACT TRUNCATED AT 250 WORDS)

Cells, Cultured↗
PubMed1991

Intraoperative infectious disease exposure to otolaryngology operating room personnel.

Health care workers are at risk of exposure to serious infectious diseases. Since the seroconversion rate is approximately 0.4% for human immunodeficiency virus and may be greater than 20% for hepatitis exposure, these risks are substantial. To assess body fluid exposure to otolaryngology operating room personnel, elective operations were prospectively analyzed over 2 months. Statistical evaluation was made between types of cases and length of procedures. Thirty-eight contaminations occurred in 228 operations with 26 torn gloves, 1 soaked grown, 6 skin scratches, 4 skin punctures, and 1 ocular exposure. Exposure was more likely in cases longer than 3 hours. Independent of procedure length, head and neck operations carried the greatest risk, followed by otologic procedures, as compared to general, endoscopic, pediatric, and elective trauma cases. The impact of potential operative exposure and universal precautions is emphasized.

Blood↗
PubMed1980

Psychiatric manifestations of sarcoidosis.

The case of an acutely depressed 34 year old man with a history of systemic sarcoidosis is presented with the question of the psychiatric manifestations of sarcoidosis in mind. The literature on Central Nervous System Sarcoidosis is reviewed and the possibility that this condition may present with psychiatric symptomatology is raised. An emphasis is placed on maintaining a high index of suspicion regarding organic factors in cases such as this, particularly in the setting of a complex, multi-system disease such as sarcoidosis.

Adult↗