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K Gloning

Publications and source records attributed to K Gloning.

At least 19 recordsLinked to original sources
PubMed2000

Current applications of single-cell PCR.

The advent of the polymerase chain reaction (PCR) has revolutionised the way in which molecular biologists view their task at hand, for it is now possible to amplify and examine minute quantities of rare genetic material: the limit of this exploration being the single cell. It is especially in the field of prenatal diagnostics that this ability has been readily seized upon, as it has opened up the prospect of preimplantation genetic analysis and the use of fetal cells enriched from the blood of pregnant women for the assessment of single-gene Mendelian disorders. However, apart from diagnostic applications, single-cell PCR has proven to be of enormous use to basic scientists, addressing diverse immunological, neurological and developmental questions, where both the genome but also messenger RNA expression patterns were examined. Furthermore, recent advances, such as optimised whole genome amplification (WGA) procedures, single-cell complementary DNA arrays and perhaps even single-cell comparative genomic hybridisation will ensure that the genetic analysis of single cells will become common practice, thereby opening up new possibilities for diagnosis and research.

Animals↗
PubMed1998

[Prenatal diagnosis with fetal cells in maternal blood: report of experiences in Basal].

Currently prenatal diagnosis relies on invasive procedures such as chorion villus sampling (CVS) or amniocentesis (AC). Many parents are reluctant to expose themselves and their child to the small, but significant risk posed by these procedures to mother and child. There is, hence, a great need for a risk-free non-invasive alternative. To achieve this goal most research has been focussed on enriching fetal cells from the blood of pregnant women. The erythroblast has emerged as the target cell of choice, since it is abundant in the early fetus, rare in normal adult blood, and since it has a very short half life, there is no risk of obtaining cells from previous pregnancies. Most enrichment protocols rely either on magnetic- or fluorescent activated cell sorting (MACS and FACS) using fetal specific antibodies. These enriched cells can be examined by FISH (fluorescence in-situ hybridisation) for the presence of the most common fetal chromosomal aneuploidies (13, 18, 21, X and Y) or by polymerase chain reaction (PCR) on singly manipulated cells for genetic disorders. The efficacy in detecting fetal aneuploidies is currently being evaluated in a phase II clinical trial under the auspices of the NIH-NICHD, the so-called NIFTY Trial, in which our group is a participant. By modifying our enrichment protocols we have recently been able to obtain detection sensitivities of almost 80%, thereby renewing our optimism that this methodology provides a solid basis for an effective non-invasive prenatal diagnostic test.

Adult↗
PubMed1989

2- and 4-iodinated estriol as indicator ligands for estriol radioimmunoassays with anti-estriol-C6 conjugate antiserum.

The suitability of 2- and 4-125I-estriol as indicator ligands for estriol determination in radioimmunoassays with anti-estriol-C6 conjugate antiserum was tested and compared to the one of 3H-estriol, estriol-6-(O-carboxymethyl)oxime-125I-histamine and a commercially available 125I-estriol derivative of unknown structure. An ideal radioiodinated tracer would react identically with the analyte and its tritiated analogue and the 4-monoiodo-estriol was found to fulfil these requirements as shown by the pattern of dilution and standard curves obtained with the various labeled ligands. This observation was corroborated by a comparison of the apparent estriol concentrations in human pregnancy sera determined with the different indicator ligands. The experimentally proven advantage of 4-monoiodo-estriol over other iodinated estriol derivatives verifies a hypothesis deduced previously from binding constants obtained with analogous estriol derivatives and the same antiserum.

Animals↗
PubMed1983

Ovulation and pregnancy after pulsatile administration of gonadotropin releasing hormone.

Twenty-seven patients with primary or secondary amenorrhea were treated with gonadotropin releasing hormone (GnRH). They were given 20 micrograms of GnRH in 50 microliter solution i.v. every 90 min by means of a peristaltic pump (Zyklomat, Ferring GmbH, Kiel). Two days after a rise in basal body temperature and when a ferning test on cervical mucus was negative the Zyklomat was removed and injections of hCG (2,500 IU) were given on alternate days until three injections had been administered. Ovulation occurred in 32 out of 40 treatment cycles and 11 patients conceived. Three of them miscarried at 6, 8, and 15 weeks of gestation. There were no severe complications of treatment and we did not encounter ovarian hyperstimulation and multiple pregnancy.

Adult↗
PubMed1983

[Pulsatile GnRH substitution in Kallmann's syndrome in women].

Anosmia and primary amenorrhoea are guiding symptoms of Kallmann's syndrome (olfacto-genital syndrome) in which agenesis of the olfactory lobe is associated with congenital defects in the mediobasal region of the hypothalamus, thus preventing a sufficient GnRH synthesis. In three patients with Kallmann's syndrome, the secretion of gonadotropins on bolus injection of 25 micrograms GnRH was comparable with prepubertal reaction. In one patient, the hypophyseal function was normalized, and ovulatory cycles were induced by pulsatile GnRH substitution via a portable computerized pump (Zyklomat). Pregnancy occurred. The duration of treatment required to induce ovulation was identical during two subsequent treatment cycles, contrary to observations in functional hypothalamic amenorrhoeas. The marked ovarian reaction shows that even if there is no endogenous GnRH secretion, a pulsatile dose of less than 20 micrograms seems to be sufficient.

Amenorrhea↗
PubMed1978

[Aphasia and neurolinguistics].

General dominance is a basis of human language. Neurolinguistics deal with this phenomenon and with central language disturbances. Collaboration with general linguistics and neuropsychology is necessary. The most important symptoms of aphasia and the problems of classification and of speech therapy are discussed.

Aphasia↗
PubMed1978

[Spatial orientation with regard to short-term memory, age and cerebral dominace].

Though orientation in acoustic space frequently has been the subject of investigations, sound was offered as a rule only in one or two dimensions of the subjective polar system of coordinates. In an earlier paper a test-situation was presented in which the offering of sound is varied in all three dimensions (horizontal and vertical angle as well as distance); the mistake also is calculated in these three dimensions separately. In this paper the investigations are continued by examinating the influence of age, the cerebral dominance and the short-term memory (offering of two different noises with following indication). Our results show that 1) age has no significant influence; 2) the relation of mistakes of the right and the left side is significantly more inhomogen in all three dimensions in left-handed persons; 3) the influence of the memory does not enlarge the mistake significantly.

Adult↗