Biomedical subjects

K Gott

Publications and source records attributed to K Gott.

22 records · Page 2Linked to original sources

PubMed1991

Experimental murine hypersensitivity pneumonitis.

To establish a model of experimental hypersensitivity pneumonitis (EHP) in mice and to examine the influence of genetic background on the pulmonary inflammatory response to Micropolyspora faeni, we determined the responses of C57BL/6, SJL/J, and C3H/HeJ mice to intratracheal (i.t.) injections of M. faeni. Recipient animals received lymph node cells (LNC), peritoneal exudate cells (PEC), and spleen cells (SC) from sensitized mice cultured in vitro with M. faeni. Controls included serum containing anti-M. faeni antibody; uncultured SC from M. faeni-sensitized donors, and M. faeni-cultured SC from ovalbumin (OA)-sensitized donors. Recipients were challenged i.t. with M. faeni or normal saline 48 hr after the cell or serum transfer. We developed a model of EHP in mice. Increasing amounts of i.t. M. faeni were associated with increasing extent of pulmonary inflammation with no difference between the mouse strains. There was substantial increase of the extent of pulmonary abnormalities in the animals receiving cultured SC. The number of transferred cells and the M. faeni concentration correlated with the extent of pulmonary histologic abnormalities. Cultured PEC and LNC could transfer EHP in C3H/HeJ mice only. Serum containing anti-M. faeni antibody, cultured SC from OA-sensitized donors, and noncultured SC from sensitized donors could not transfer EHP. We conclude that it is possible to adoptively transfer EHP.

Actinomycetales↗

PubMed1991

Mast cell hyperplasia in experimental hypersensitivity pneumonitis.

We investigated the presence of mast cells in a model of experimental hypersensitivity pneumonitis (EPH). Guinea pigs exposed to 8 weekly intratracheal challenges with Micropolyspora faeni exhibited significant increases in the number of mast cells within the lung as compared to controls and animals challenged only 2 or 4 times. The number of cells in M. faeni-challenged animals were increased around bronchi, bronchioles, blood vessels and in alveolar septa. There appeared to be contraction of peribronchial, peribronchiolar and vascular smooth muscle. Ultrastructural examination of lung tissue revealed the presence of degranulating mast cells. Bronchoalveolar lavage histamine levels were increased after 8 but not after 2 or 4 weekly challenges. Serum anti-M. faeni antibody was present in all M. faeni-exposed animals but not in control animals. We conclude that mast cells and histamine levels in bronchoalveolar lavage fluid are increased in a model of EHP caused by repetitive, intratracheal injection of M. faeni particulate antigen.

Alveolitis, Extrinsic Allergic↗

PubMed1990

Experimental hypersensitivity pneumonitis: influence of donor sensitization.

Experimental hypersensitivity pneumonitis (EHP) can be transferred to strain 2 guinea pigs by lymphoblasts from lymph node cells from sensitized guinea pigs cultured in vitro with antigen. We sought to examine the relationship between characteristics of the donor animal and development of competence to transfer EHP. We also compared cell populations that were capable and incapable of transfer using flow cytometry and fluorescein conjugated anti-Ig to determine cell size and surface IgG + (SIg +: surface immunoglobulin-positive) cells. Lymph node cells from donor animals were cultured with a soluble extract of Micropolyspora faeni (10 micrograms/ml) for 72 hours; blasts were then isolated and transferred intravenously to syngeneic recipients. Control recipients received an equal volume of medium. Four groups of donors were used: animals systemically sensitized with Freund's adjuvant and M. faeni and challenged with two, four, or eight weekly intratracheal injections of M. faeni (2-, 4-, and 8-week group); and animals sensitized with Freund's adjuvant and normal saline and challenged with two weekly intratracheal injections of normal saline (NS group). Recipients were challenged intratracheally with M. faeni 48 hours after the cell transfer and killed 4 days thereafter. Randomly selected microscopic fields of the lung (250/animal) were judged to be normal or abnormal without knowledge of treatment. All animals were maintained in high efficiency particulate accumulator-filtered air. There was a low level of pulmonary response to an intratracheal challenge of M. faeni in animals that received media.(ABSTRACT TRUNCATED AT 250 WORDS)

Actinomycetales Infections↗

PubMedDate not supplied

Experimental hypersensitivity pneumonitis: suppressor cell influences.

Experimental hypersensitivity pneumonitis (EHP) can be transferred to strain 2 guinea pigs by lymph node cells (LNC) cultured in vitro with antigen. Using mixtures of cell populations, we sought to determine if functional suppressor cells were present in our system. We also characterized the composition of cell populations that were capable (blast 10 micrograms/mL Micropolyspora faeni from 2-week donor animals) and incapable (blast 0 micrograms/mL M. faeni from 2-week donor animals; blast 10 micrograms/mL from 8-week donor animals) using flow cytometry, anti-Ig and monoclonal antibody 8BE6 (T cell marker) of transferring EHP. Two groups of donors were used: animals sensitized with Freund's adjuvant and M. faeni and challenged with either two or eight weekly intratracheal (IT) injections of M. faeni (2- and 8-week groups). LNC from donor animals were cultured with a soluble extract of M. faeni (10 or 0 micrograms/mL) blasts isolated and transferred IV to syngeneic recipients. Control animals received media IV. Recipients were challenged IT with M. faeni 48 h after the cell transfer and sacrificed 4 days thereafter. All animals were maintained in HEPA filtered air. Randomly selected microscopic fields of the lung (250/animal) were judged to be normal or abnormal without knowledge of treatment. There was a low level of pulmonary response to an IT challenge of M. faeni in media recipients. There was a substantial increase (P less than .01) in pulmonary abnormalities in the animals receiving blasts from the 10-micrograms/mL M. faeni 2-week group. Addition of cells from incompetent cell populations (0 micrograms/mL M. faeni 2-week donors or 10 micrograms/mL M. faeni 8-week donors) did not alter the ability of competent populations to transfer EHP. Cells cultured with antigen had a decreased proportion of T cells and an increased proportion of SIg+ and large cells. Competent and incompetent cell populations did not differ in regard to proportion of large cells, surface Ig+, or T cells. We conclude that the inability of certain cell populations to transfer EHP is not associated with the appearance of functional suppressor cells. Differences of ability to transfer EHP do not correlate with differences of size distribution or T and B cell composition.

Alveolitis, Extrinsic Allergic↗