PubMed Health⌕ Search

Biomedical subjects

K Gulewicz

Publications and source records attributed to K Gulewicz.

10 recordsLinked to original sources
PubMed2001

Equilibrium between basic nitrogen compounds in lupin seeds with differentiated alkaloid content.

The results of studies on the content of the nitrogen basic compounds, viz. quinolizidine alkaloids, biogenic polyamines and basic amino acids in lupin seeds are presented. The investigations concerned three lupin species (Lupinus angustifolius L., Lupinus albus L. and Lupinus luteus L.) and 10 bitter and sweet cultivated varieties. Content of quinolizidine alkaloids in L. angustifolus ranged from 11.4 to 19.6 microg mg(-1) dw (bitter cultivars), from 0.18 to 0.47 microg mg(-1) dw (sweet), in L. albus from 0.58 microg mg(-1) dw (sweet) to 29.6 microg mg(-1) dw (bitter) and in L. luteus from 0.59 (sweet) to 14.7 microg mg(-1) dw (bitter). Total biogenic polyamine content ranged in L. angustifolius from 2,773.9 to 3,180.2 pmol mg(-1) dw (bitter) and from 315.0 to 599.0 pmol mg(-1) dw (sweet), in L. albus from 432.6 pmol mg(-1) dw (sweet) to 1,832.0 pmol mg(-1) dw (bitter) and in L. luteus from 506.9 pmol mg(-1) dw (sweet) to 2,091.8 pmol mg(-1) dw (bitter). Total basic amino acids varied in L. angustifolus from 1,034.3 to 1,704.6 pmol mg(-1) dw (bitter) and from 1,761.9 to 2,101.9 pmol mg(-1) dw (sweet), in L. albus from 696.9 pmol mg(-1) dw (bitter) to 1,269.2 pmol mg(-1) dw (sweet) and in L. luteus from 927.6 pmol mg(-1) dw (bitter) to 1,598.3 pmol mg(-1) dw (sweet). We found a close dependence between alkaloid content and level of biogenic polyamines and basic amino acids in all three lupin species tested. All bitter lupin seeds also contain high level of biogenic polyamines but a low content of basic amino acids. The reverse relationship in sweet lupin seeds was found. The findings demonstrate that lupin nitrogen basic compounds are in steady equilibrium and that change of content in one compound leads to corresponding change in the content of another.

Alkaloids↗
PubMed2000

Antifungal activity of Bacillus sp. isolated from compost.

Four strains of Bacillus isolated from lupine compost exhibited an antifungal activity against six plant fungal pathogens (Rhizoctonia solani, Bipolaris sorokiniana, Sclerotinia sclerotiorum, Trichothecium roseum, Fusarium solani, Fusarium oxysporum). It was significantly influenced by the composition of the cultivation media.

Animals↗
PubMed2000

Simple method of isolation and purification of alpha-galactosides from legumes.

A simple method for the isolation and purification of alpha-galactosides, raffinose family oligosaccharides (RFOs), from legumes has been developed. The method includes (i) imbibition of seeds, (ii) extraction with 50% ethanol, (iii) precipitation of RFOs, (iv) purification of RFOs on diatomaceous earth and charcoal, and (v) cation-exchange chromatography. The described method allows one to obtain high purity RFO preparations (90% for lentil and 80% for pea seeds, determined by HPLC-RI analysis) in the form of white, fine powder. Yields of alpha-galactosides isolated from 100 g of seeds of lentil and pea were 5.6 and 4.3 g, respectively.

Chromatography, High Pressure Liquid↗
PubMed1994

Immunotropic activity of lupin seeds extracts and fractions from Lupinus angustifolius and Lupinus albus.

The results demonstrated immunotropic activity of seeds extracts and fractions from Lupinus angustifolius and Lupinus albus. Plaque forming cells (PFC) number to SRBC (sheep red blood cells) were elevated by an extract from Lupinus angustifolius and lowered by extracts from Lupinus albus. All preparations obtained from Lupinus angustifolius reduced the number of rosette forming cells (E-RFC). These preparations suppressed also the intensity of graft versus host reaction (GVHR) in case when the donors were treated. Lupinus albus extract suppressed GVH reaction when recipients were treated. Lupin extracts stimulated draining popliteal lymph nodes in PLN assay.

Adjuvants, Immunologic↗
PubMed1994

Gibberellic acid production by Fusarium moniliforme on lupin seed extract.

Fusarium moniliforme grown on extract obtained from lupin (Lupinus angustifolius L.) seeds effectively produced gibberellic acid (GA3). The extract used at the concentration of 8.3-0.41% d.w. inhibited the fungal growth. As much as 0.35-0.40 g of GA3 per liter was produced on the 2% extract. The latter medium competes effectively with other media used so far for GA3 production.

Alkaloids↗
PubMed1981

The reaction of adenine and cytosine residues in tRNA with chloroacetaldehyde.

The reaction of Lupinus luteus tRNAPhe with 1 M chloroacetaldehyde in the pH range of 4 - 6 at 25 degrees C was studied. It was found that earlier difficulties lowering the utility of the reagent for structural studies of nucleic acids were caused by the formation of the stable reaction intermediates. In order to eliminate these difficulties the simple procedure of so-called maturation of the chloroacetaldehyde-modifies tRNA is proposed. It consists in further incubation of the short-term modified tRNA in water at 50 degrees C in the absence of the reagent. During the maturation step the stable intermediates are quantitatively converted into final ethenoderivatives. New HPLC conditions on Aminex A-6 were worked out which enable rapid separation of both ethenoadenosine and ethenocytidine from natural tRNA nucleosides.

Acetaldehyde↗
PubMed1981

Properties of native and nicked elongation factor Tu from Thermus thermophilus HB 8.

Two alternative procedures for the isolation of the elongation factor Tu from Thermus thermophilus were compared and the properties of a specifically nicked EF-Tu . GDP were examined in detail. Although the native elongation factor possessed similar catalytic activities in all reactions investigated as the protein isolated by Arai et al. [Eur. J. Biochem. 92, 509-519 and 521-531 (1978)] it could not be crystallized. The nicked EF-Tu, consisting of two associated fragments with molecular weights of 41000 and 8000 respectively, was active in binding GDP, GTP and in the formation of Phe-tRNAPhe . EF-Tu . GTP ternary complex. However, it did not promote poly(U)-dependent synthesis of polyphenylalanine on Escherichia coli ribosomes. The isolated fragment of a molecular weight of about 41000 did not bind GDP. This activity could be reconstituted with the supplement of the small 8000-Mr fragment. It is demonstrated that, in contrast to the native EF-Tu, the nicked EF-Tu forms high-molecular-weight aggregates. Cleavage of the polypeptide chain of EF-Tu from T. thermophilus stimulates the crystallization of this protein.

Chromatography, Gel↗
PubMedDate not supplied

The toxicity of seed extracts and their fractions from Lupinus angustifolius L. and Lupinus albus L.

Seed extracts obtained from Lupinus albus and Lupinus angustifolius by treatment with 48% ethanol contained ca. 10% alkaloids (on a dry weight basis) and were non-toxic. Their acute toxicity (LD50) in the mouse is > 4000 mg kg-1 body wt. After fractionation, the extract from L. angustifolius seeds afforded several fractions with differing toxicities (LD50 750-4000 mg kg-1 body wt.). None of the fractions tested in vitro were toxic. The results obtained showed that, in spite of the alkaloids, other low-molecular-weight constituents present significantly modified the toxicity of the lupin extracts.

Alkaloids↗