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Biomedical subjects

K H Tiedemann

Publications and source records attributed to K H Tiedemann.

5 recordsLinked to original sources
PubMed1991

Detection of human papillomaviruses in paraffin-embedded condylomata acuminata--comparison of immunohistochemistry, in situ hybridization, and polymerase chain reaction.

The polymerase chain reaction (PCR) is used for detection of human papillomavirus (HPV) DNA in paraffin-embedded tissue sections of condylomata acuminata. Incorporation of biotinylated nucleotides during the amplification process allows a highly sensitive, fast, and non-isotopic detection of viral DNA in a subsequent Southern dot blot. In 100% (13 of 13) of histologically confirmed condylomata, HPV-6 or -11 could be detected by polymerase chain reaction. By in situ hybridization 77% (10 of 13) and by immunohistochemistry (IHC) 69% (nine of 13) positive results were obtained. Because HPV genital infection is linked to penile and cervical dysplasia, polymerase chain reaction provides a powerful and highly sensitive tool for epidemiologic studies on sexual transmission of HPV.

Adult↗
PubMed1988

Release of a virus coded glycoprotein from herpes simplex virus type 1 infected cells.

HEp-2 cells, which were infected with HSV-1, excrete besides other proteins a soluble glycoprotein (Mr 125,000-130,000) related to the virus protein gC. The excretion of the glycoprotein and the production of extracellular virus particles is reduced to a similar extent when the cells were treated with monensin. Possible consequences of the excretion of soluble viral proteins to a modulation of the immune response are discussed.

Cells, Cultured↗
PubMed1986

[Cholecystitis in accident surgery].

Out of a large number of cholecystectomies four cases are reported. In some cases, the gallbladder was involved in the traumatic lesion itself but there are also cases, in which the cholecystitis developed independently of the primary lesion. Beside abdominal trauma which leads to a disruption of the organ wall we also see an inflammatory disease of the gallbladder after perforation during liver blind punction. In another case a patient with gall stone history developed acute cholecystitis after operative treatment of a collum femuris fracture. The fourth case shows acute cholecystitis which also arose after operative treatment of bone fracture in an acalculous bile vesicle. Acute cholecystitis after severe trauma not inflicting the organ itself is a severe, life threatening complication. One step to acute acalculous cholecystitis is the sludge formation in the gallbladder which can be recognized by ultrasonography. The pathogenetic mechanisms which lead to sludge phenomenon and later to acute cholecystitis are multifactorial. Some theories which try to explain this phenomenon are discussed. In each case the development of cholecystitis after trauma is a dangerous complication which urgently demands nonconservative treatment.

Adult↗