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Biomedical subjects

K H Weber

Publications and source records attributed to K H Weber.

At least 37 records · Page 2Linked to original sources
PubMed1983

Chemical structure and biological activity of the diazepines.

Since the introduction of chlordiazepoxide and diazepam many diazepines have been developed. Use of these drugs is increasing and considerable knowledge has accumulated about their mechanisms of action. The structural and pharmacological properties of these drugs are surveyed briefly.

Acetylcholine↗
PubMed1979

Acetylcholine receptor. Binding properties and ion permeability response after covalent attachment of the local anaesthetic quinacrine.

Membrane vesicles rich in nicotinic acetylcholine receptor prepared from Torpedo californica electric tissue have been irreversibly modified with quinacrine mustard, an alkylating derivative of the local anaesthetic quinacrine. The reaction blocked the ion channel regulated by the acetylcholine receptor. Acetylcholine still bound to the modified membrane vesicles with KD approx. 10(-8). The number of binding sites was reduced by up to 50%. Stopped-flow experiments showed that in contrast to what had been found with the reversibly binding quinacrine no fluorescence changes caused by energy transfer from the irradiated protein to the fluorescent local anaesthetic occurred after addition of agonist. This indicates that the conformational changes associated with the activation of the ion channel are blocked by the covalent reaction with quinacrine mustard. Analysis of the membrane vesicles by SDS-polyacrylamide gel electrophoresis showed that all polypeptide chains assumed to be part of the receptor complex had reacted with the mustard. Even small components, probably lipids, migrating with the dye front, showed fluorescence.

Animals↗
PubMed1975

[Macro-amylasaemia: report of two cases (author's transl)].

Macro-amylasaemia is a rare form of hyper-amylasaemia without evidence of pancreatic or renal disease. It is due to the presence of a macro-molecular amylase complex in serum, whose origin and clinical significance remains uncertain. The abnormality was diagnosed in a 25-year-old woman and a 44-year-old man. In both the amylase complex was demonstrated by gel filtration on Sephadex G-200.

Adult↗