Biomedical subjects

K Hald

Publications and source records attributed to K Hald.

3 recordsLinked to original sources

PubMed2002

Characterization of an enzyme-linked immunosorbent assay for soluble CD163.

We have recently identified a soluble plasma form of CD163 sCD163, the macrophage receptor for clearance of haptoglobin-haemoglobin complexes, and we have observed highly elevated levels of sCD163 in subgroups of haematological patients. In the present study, we describe the optimization and characterization of a sandwich ELISA for the determination of the concentration of sCD163 in plasma and serum. The optimal concentrations of antibodies were determined systematically and the assay was calibrated by CD163 purified from human spleen membranes. The minimum detection limit was below 6.25 microg/L. Recovery of CD163 added to plasma samples was 106%. The concentration of sCD163 in paired serum and plasma samples correlated well (r2=0.99); however, serum levels were 1.1 times higher than the plasma levels. The addition of haptoglobin-haemoglobin complexes did not influence the assay. A very high stability of sCD163 was measured in whole blood and in plasma subjected to different temperatures and after repeated freezing and thawing.

Antibodies↗

PubMed2000

Three dimensional alignment of molecules using elliptically polarized laser fields

We demonstrate, theoretically and experimentally, that an intense, elliptically polarized, nonresonant laser field can simultaneously force all three axes of a molecule to align along given axes fixed in space, thus inhibiting the free rotation in all three Euler angles. Theoretically, the effect is illustrated through time dependent quantum mechanical calculations. Experimentally, 3, 4-dibromothiophene molecules are aligned with a nanosecond laser pulse. The alignment is probed by 2D ion imaging of the fragments from a 20 fs laser pulse induced Coulomb explosion.

Journal Article↗

PubMed1989

[Requests for ambulances for patients with acute diseases and injuries. Are ambulance services abused?].

The aim of this study was to describe the dispatch of emergency ambulance requests in Trondheim and to evaluate the medical justification of such ambulance missions. Data concerning 555 emergency ambulance missions were recorded from March to August 1986. A review of the cases shows that almost 40 per cent were unjustified. However, whether the ambulance missions were justified or not was significantly connected to the age of the patient and with the person requesting the ambulance. Missions requested by health care professionals and missions to elderly patients were the most justified ones. Furthermore, the mission was more justified in the case of internal medical problems than for surgical cases and trauma. We also discuss different ways of improving the use made of emergency medical services outside hospital.

Ambulances↗