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Biomedical subjects

K Hashimoto

Publications and source records attributed to K Hashimoto.

At least 829 records · Page 46Linked to original sources
PubMed1996

Internal DNA deletion within the BCL-6 gene on untranslocated chromosome in non-Hodgkin's lymphoma with 3q27 abnormality.

Chromosomal translocations involving the band 3q27 are recognized as common specific cytogenetic abnormalities in B cell non-Hodgkin's lymphoma (NHL), and the BCL-6 gene, identified on 3q27 was shown to be disrupted by these translocations. Previously, we have reported biallelic BCL-6 rearrangements occurring in some patients with B cell NHL. In the present study, we describe a NHL patient with t(3;22)(q27;q11) translocation. In this patient, biallelic BCL-6 abnormalities were indicated by Southern blot analysis. Further studies revealed that one of the two independent abnormalities was a juxtaposition to the immunoglobulin (Ig) lambda gene associated with chromosomal translocation, whereas the other was an internal DNA deletion of 1.5 kb area on untranslocated chromosome 3. Deletion junctions were located within the first exon and the 5' region of the first intron. The result provides the evidence that, besides chromosomal translocation, submicroscopic local DNA recombination can cause structural alteration of the BCL-6 gene.

Base Sequence↗
PubMed1996

[An unusual autopsy case of corticobasal degeneration--with special reference to clinicopathological differentiation from progressive supranuclear palsy and slowly progressive aphasia].

We report an unusual autopsy case of corticobasal degeneration. The patient was male who was 67 years old at the time of his death. He developed clumsiness of his right hand at age 65. Neurological examination at age 66 revealed supranuclear ophthalmoplegia of upward gaze and parkinsonism. Progressive supranuclear palsy was suspected. The patient's clinical course was rapidly progressive. Dementia developed nine months after the onset of the disease, and the patient manifested apallic syndrome at 21 months and died of pneumonia at about 25 months. At autopsy the brain weighed 1370g. Macroscopic examination revealed prominent atrophy of the anterior and parietal lobes, particularly of the pars opercularis in the left inferior frontal gyrus, and there was marked depigmentation of the substantia nigra. Histological examination showed neuronal loss, glial proliferation, spongy state, and ballooned neurons in the frontal and parietal lobes. Neuronal loss with glial proliferation was conspicuous in the striatum, pallidum, thalamus, and substantia nigra. There was slight neuronal loss in the dentate nucleus and locus ceruleus. Staining by Gallyas-Braak method revealed massive appearance of argyrophilic threads. This case is important in terms of the clinical differentiation from progressive supranuclear palsy, and is also interesting in regard to the pathological correlation with slowly progressive aphasia.

Aged↗
PubMed1996

[Usefulness of estriol for the treatment of bone loss in postmenopausal women].

To assess the effect of estriol (E3) on bone loss in Japanese postmenopausal women, twenty-five women within 10 years after menopause received 2 mg/day of E3 and 2 g/day of calcium lactate (Ca) daily (n = 17; Group-E3 + Ca) or 2 g/day of Ca daily (n = 8; Group-Ca) for one year. Bone mineral density (BMD) at the lumbar spine L2 - L4 was evaluated by dual energy X-ray absorptiometry (DXA). In Group-Ca, BMD was significantly reduced (p < 0.05 vs. pretreatment) at the end of the study period. In contrast, in Group-E3 + Ca, the mean percent increase in BMD was 1.66%, which was significantly higher than that of Group-Ca (-3.08%; p < 0.001). As for the biochemical markers of bone metabolism, bone specific alkaline phosphatase (ALPIII) reflecting bone formation, and pyridinoline (Pyr) and deoxypyridinoline (D-Pyr) reflecting bone resorption, were significantly decreased (p < 0.001) in Group-E3 + Ca after six months of treatment. These data indicate that the acceleration of bone turnover usually observed after menopause was prevented by treatment with E3. As to the side effects of E3, only one of the 17 patients experienced a slight increase in vaginal discharge, but no other side effects were observed. Accordingly, reasonable compliance can be expected among postmenopausal women treated with E3 to prevent bone loss.

Absorptiometry, Photon↗
PubMed1996

Merkel cells participate in the induction and alignment of epidermal ends of arrector pili muscles of human fetal skin.

The follicular proximal ends of arrector pili muscles anchor the bulge areas of human hair follicles. However, the location of the ends of these muscles towards the epidermis, especially the fine points of attachment, is not fully understood. Conventional serial sections of the scalp skin of a 12-month-old infant were examined. Most of the distal ends were situated in the upper dermis, while some seemed to be in close contact with the epidermal basal layers. In vertical sections of the scalp skin of 36-week-old fetus, double immunoenzyme staining showed a close topographic correlation between the epidermal ends of the arrector pili muscles and the epidermal and dermal Merkel cells. These findings allow speculation that fetal Merkel cells participate in the induction and alignment of arrector pili muscles.

Fetus↗
PubMed1996

Unilateral facial angiofibromas--a segmental form of tuberous sclerosis.

Multiple facial angiofibromas are thought to be a pathognomonic and common, feature of tuberous sclerosis. In contrast, it is rare to see multiple angiofibromas limited to one side of the face. We have studied a patient with multiple angiofibromas of one cheek, in order to search for other manifestations of tuberous sclerosis and to determine the histochemical identity of the angiofibromas. No evidence of other pathology known to be associated with the tuberous sclerosis complex was uncovered. Histopathology of the unilateral lesions revealed focal fibroplasia and positive cellular staining for factor XIIIa and vimentin, similar to that described for bilateral angiofibromas. We suggested that the segmental expression of tuberous sclerosis, in the form of unilateral facial angiofibromas, may result from a postzygotic mutation.

Adult↗
PubMed1996

Cutaneous Pneumocystis carinii and Cryptococcus neoformans in AIDS.

A patient with AIDS was found to have skin lesions which contained the organisms Pneumocystis carinii and Cryptococcus neoformans. Both organisms were identified using haematoxylin and eosin, Gomori methenamine silver, Giemsa and mucicarmine stains. Electron microscopy was performed and confirmed their presence. The clinical presentation, histopathology, differential diagnosis and treatment of cutaneous P. carinii and C. neoformans in AIDS is presented.

AIDS-Related Opportunistic Infections↗
PubMed1996

[Primary transitional cell carcinoma of the prostatic urethra: a case report].

A case of primary transitional cell carcinoma of the prostatic urethra is reported. A 60 year-old male, with a chief complaint of difficult urination consulted our clinic on March, 1994. Urethrography and endoscopic examination revealed the solitary tumor of the prostatic urethra and transurethral resection was performed. Histologically, the tumor was Grade 2 transitional cell carcinoma without submucosal invasion. The post-operative course was uneventful and no recurrence has been seen. To our knowledge, including our case, 10 cases of primary transitional cell carcinoma of the prostatic urethra have been reported in the Japanese literature.

Carcinoma, Transitional Cell↗
PubMed1996

Pedunculated proliferative papillomatosis.

Skin tags (soft fibromas) are benign connective tissue neoplasms of the dermis. As a rule they occur as soft, skin-colored, filiform, often pedunculated growths in intertriginous areas. The classification and naming of these lesions is somewhat arbitrary due to their banal clinical and histological spectrum of characteristics. We report an unusual case of a peculiar proliferation of dermal and epidermal components that clinically and histologically appears to fit into this category of soft fibroma.

Buttocks↗
PubMed1996

[A case of renal cell carcinoma presenting as spontaneous renal rupture].

A case of renal carcinoma presenting as spontaneous renal rupture is reported. A 50-year-old man was admitted to our hospital with the chief complaint of left lumbal pain. There was no evidence of recent trauma. Intravenous pyelography showed extravasation of perirenal area and space-occupying lesion at the left upper pole of the kidney. Left perirenal hemorrhage was found on computed tomography (CT scan). Suspecting extracapsular hemorrhage caused by rupture of the renal tumor, we performed nephrectomy. Histological diagnosis was renal cell carcinoma, alveolar type, clear cell subtype, grade 2, INF alpha, pT2. The patient has been well without local recurrence or distant metastasis for 9 months after the operation. To our knowledge, this is the 16th case or renal cell carcinoma with spontaneous rupture, reported in the Japanese literature, and the 7th case of extracapsular renal rupture.

Antineoplastic Agents↗
PubMed1996

[Two cases of recurrent, massive hemoptysis with contralateral, concurrent hemoptysis].

Bilateral, concurrent massive hemoptysis with respiratory failure is rare and difficult to manage. We encountered two patients with this condition. One was a 51-year-old woman and one was a 63-year-old woman. Both had inactive pulmonary tuberculosis and episodes of hemoptysis. Home oxygen therapy was prescribed to the former patient and bronchial artery embolization was done in the latter. The sites of initial bleeding were in the left basal segment and in the lingular segment. Bleeding was well controlled by endobronchial tamponade with a Fogarty balloon catheter in the left main bronchus, contralateral intubation, and systemic artery embolization. Use of water instead of air in the balloon was more effective in over the long term. Concurrent bleeding from the contralateral upper lobe bronchus was controlled by compression with a tracheal tube cuff at the bronchial orifice. Afterward, an infiltrative shadow was seen on the chest X-ray film and hemoptysis occurred in case 2 occurred in case 2. Treatment with erythromycin and antituberculosis drugs brought about improvement of chest X-ray findings and disappearance of the hemoptysis.

Antitubercular Agents↗
PubMed1996

[Evaluation of postoperative pain in spinal surgery--usefulness of the administration of local anesthetics into the peridural cavity].

Analgesic effects of perioperative administration of local anesthetics into the peridural cavity were examined in 21 patients with cervical or lumbar vertebral diseases. Sufficient analgesic effects were obtained particularly in patients with lumbar vertebral disease. Administration of local anesthetics was also effective for postoperative sleep on the day of operation. No postoperative neurological side effects were observed. These findings suggest that peridural administration of local anesthetics during spinal surgery is very useful.

Adult↗
PubMed1996

[Diagnosis and prognosis of periventricular leukomalacia].

Periventricular leukomalacia (PVL) is an important pathogenetic factor in cerebral palsy in premature infants. We studied 708 premature infants with gestational ages < 36 weeks, who had been admitted to the NICU of Matsudo City Hospital, examined by cranial ultrasonographic method repeatedly, and followed up for more than 1 year with cranial ultrasonography, magnetic resonance imaging (MRI), and CT scanning. We made a diagnostic protocol for PVL. All cystic lesions and periventricular echo densities (PVE) 3, and 14.9% of PVE 2 on ultrasonography were clinically diagnosed in PVL. Cranial MRI were consistent with clinical symptoms, and its complicated findings corresponded to the severity of PVL. This protocol was useful for the diagnosis of clinical PVL.

Echoencephalography↗
PubMed1996

Free thyroxine concentrations in serum measured by equilibrium dialysis in chronic renal failure.

The serum concentration of free thyroxine (FT4) is often low in patients with chronic renal failure (CRF) with low serum concentrations of triiodothyronine (T3). We evaluated the serum FT4 concentration by using both an equilibrium dialysis RIA kit (D-FT4) and a labeled-antibody kit (M-FT4) in two different groups of CRF patients, undergoing chronic hemodialysis (HD, n = 145) or not (non-HD, n = 30), and in a group of normal healthy subjects (n = 58). Thyroid peroxidase antibodies and thyroglobulin antibodies were not detected in any patient. Serum FT4 concentrations (mean +/- SD, pmol/L) by the D- and M-FT4 assays were, respectively, 21.5 +/- 4.6 and 16.6 +/- 2.0 in the healthy subjects, 17.8 +/- 4.3 and 13.9 +/- 3.6 in the non-HD patients, and 16.9 +/- 4.9 and 10.7 +/- 1.9 in the HD patients. By the D-FT4 assay, results for both CRF groups were significantly different from those for the healthy group (P <0.01), as were the results for each pair of groups by the M-FT4 assay (P <0.01). FT4 values were reported as being within the healthy reference range by D-FT4 in 73 of 113 HD subjects who had low T3 and low M-FT4 values. Serum FT4 concentrations measured by both assay kits showed a significant inverse correlation with the serum concentration of creatinine (P <0.01), but the serum concentrations of sex-hormone-binding globulin did not differ significantly among the three groups. Our results indicate that the low FT4 concentration measured by D-FT4 in patients with CRF, particularly those on HD, probably reflects the actual, mild nonthyroidal illness of renal failure.

Adult↗
PubMed1996

[Epileptic vertiginous seizure in a Japanese boy: a case report].

Dizziness in childhood is not an infrequent symptom, but epileptic vertigo is a rare condition in children. Here we report an 8-year-old Japanese boy with epileptic vertiginous seizures. At age 8 years, he visited Nippon Medical School Hospital because of a ten day history of dizziness. The dizziness occurred more than twenty times a day and he was hospitalized. On physical examination, the patient appeared normal and there were no abnormal neurological findings, including eye movement and cerebellar signs. Ophthalmoscopy, otoscopy, vestibular function test and hearing test were normal. Computerized tomography scanning and MR imaging of the head revealed no significant abnormality. The dizziness observed on admission comprised sudden brief attacks of rotatory sensation without amnesia regarding the event. Sometimes the attacks were accompanied by tremor like movement and numbness of the right hand, followed by postictal unsteadiness. Interictal EEG revealed spike-and-wave complexes in the central region dominantly in the light sleep stage. On ictal EEG, seizure discharges were observed to begin in the left central region and they increased in amplitude and subsequently propagated to the frontal and occipital regions. These findings were most suggestive of partial seizures. The patient was treated with carbamazepine and the seizures became well under control.

Anticonvulsants↗
PubMed1996

Long-term culture of avian embryonic cells in vitro.

The pH of the embryonic blood, one of the most important environmental factors for embryonic cells, was found to range from 8.1 to 8.5 in chick embryos until 108 h after incubation. Based on these results, the culture medium adjusted to pH 8.0 was used to culture embryonic chick and quail cells. They were easily subcultured for a long period of time at pH 8.0. This pH culture condition may have wide application for manipulating embryonic cells or tissues and establishing cell lines from avian embryos.

Animals↗