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Biomedical subjects

K Hasiba

Publications and source records attributed to K Hasiba.

2 recordsLinked to original sources

Inactivation of Escherichia coli by ultrasonic irradiation.

Ultrasonic inactivation of Escherichia coli XL1-Blue has been investigated by high-intensity ultrasonic waves from horn type sonicator (27.5 kHz) utilizing the "squeeze-film effect". The amplitude of the vibration face contacting the sample solution was used as an indication of the ultrasonic power intensity. The inactivation of the E. coli cells by ultrasonic irradiation shows pseudo first-order behavior. The inactivation rate constant gradually increased with increasing amplitude of the vibration face and showed rapid increase above 3 microm (p-p). In contrast, the H2O2 formation was not observed below 3 microm (p-p), indicating that the ultrasonic shock wave might be more important than indirect effect of OH radicals formed by ultrasonic cavitation in this system. The optimal thickness of the squeeze film was determined as 2 mm for the E. coli inactivation. More than 99% of E. coli cells was inactivated within 180-s sonication at the amplitude of 3 microm (p-p) and 2 mm of the thickness of the squeeze film.

Escherichia coli↗

[Self-injury behavior].

BACKGROUND: Self-damage is defined as intentional injury of the own body. Patients with this disorder often consult and deceive surgeons. In case of factitious disease the diagnosis of self-injurious behavior can be difficult. METHODS: A literature review on self-injuring behavior was done with special emphasis on its clinical presentation in surgical departments, its psychodynamic background and the therapeutic consequences. RESULTS: Self-damaging behavior is most frequent in adolescent females. Both a disturbed relation with the own body and with fellow-beings is the problem of all patients. Deprivation, physical or sexual abuse are common in the biography of these patients. The feelings of internal emptiness and unbearable psychic tension are the immediate psychodynamic causes of the self-damaging act. Psychotherapeutic strategies are aimed at learning to express emotions in a better way, to care for the own body, and to establish confidential and stable relationships. CONCLUSIONS: The knowledge of the psychodynamic background facilitates the therapeutic approach to patients with self-injuring behavior.

Adolescent↗