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Biomedical subjects

K Hosoi

Publications and source records attributed to K Hosoi.

At least 109 records · Page 6Linked to original sources
PubMed1981

Induction of various androgen-dependent esteroproteases (trypsin-like and chymotrypsin-like enzymes) by tri-iodo-L-thyronine in the submandibular glands of female mice and mice with testicular feminization.

Trypsin-like and chymotrypsin-like esteroprotease isozymes of the mouse submandibular gland were separated by isoelectric focusing. In normal female mice the following pI-isozyme activities were found; pI-4.6, -5.6 (shoulder), -5.8, -7.1, and -9.9, hydrolytic activities for benzoylarginine ethylester (BAEE) (trypsin-like enzymes), and pI-4.7 and -10.3 hydrolytic activities for acetyltyrosine ethylester (ATEE) (chymotrypsin-like enzymes). In mice with testicular feminization (Tfm mice), only pI4.6 hydrolytic activity for BAEE was found; no ATEE hydrolytic activity was detected. In normal female mice, both 5 alpha-dihydrotestosterone (5 alpha-DHT) and tri-iodo-L-thyronine (T3) significantly increased all these isozymes except the pI-4.6 hydrolytic activity for BAEE. In Tfm mice, T3 also increased all these isozymes except the pI-4.6 hydrolytic activity for BAEE, but 5 alpha-DHT had no effect on any enzymes. These results suggest that the pI-4.6 hydrolytic activity for BAEE is non-inducible by the two hormones. Androgen does not seem to be involved in the inductions of these esteroproteases by T3.

Androgen-Insensitivity Syndrome↗
PubMed1981

Induction of epidermal growth factor by tri-iodo-L-thyronine in the submandibular glands of mice with testicular feminization.

The content of epidermal growth factor (EGF) as a high molecular weight complex (HMW-EGF) in the submandibular glands of mice was measured simply by a single radial immunodiffusion method. In female mice, the amount of HMW-EGF was increased 10-fold by tri-iodo-L-thyronine (T3) and 60-fold by 5 alpha-dihydrotestosterone (5 alpha-DHT). In mice with testicular feminization (Tfm), which are genetically deficient in androgen receptor, T3 but not 5 alpha-DHT increased the HMW-EGF from a non-detectable level to 5.4 +/- 0.94 micrograms/mg protein. It was concluded that EGF is also synthesized under the control of thyroid hormone in vivo, and that androgen was not involved in this induction of EGF by thyroid hormone.

Androgen-Insensitivity Syndrome↗
PubMed1981

[A developmental study of narcissistic tendency (1) : women in pregnancy and confinement (author's transl)].

This study examined developmental changes in the narcissistic tendency in women by two questionnaires. The first questionnaire was given to 235 women in pregnancy (P groups) and the second to 49 women in confinement (C group) who were randomly chosen from among the 235 women surveyed first. Women when pregnant were more sensitive and more narcissistic compared with when not pregnant. The narcissistic tendency associated with Exhibition found for adolescent non-pregnant women (Hosoi, 1978 a) was also found for the women in pregnancy, with addition of association with Ego Ideal. They were also concerned with themselves more than their fetus.

Adolescent↗
PubMed1980

Effect of autonomic agents on the secretion of N-acetyl-beta-glucosaminidase of mouse submaxillary gland.

The secretion of N-acetyl-beta-glucosaminidase of mouse submaxillary gland into saliva was stimulated by norepinephrine and phenylephrine but not by pilocarpine and isoproterenol. The stimulative effects of the alpha-adrenergic agents were inhibited by alpha-blockers, phentolamine, and phenoxybenzamine. These results suggest that the secretion of the enzyme is regulated through alpha-adrenergic receptors.

Acetylglucosaminidase↗
PubMed1979

Androgenic regulation of N-acetyl beta-glucosaminidase activity in the submandibular glands of mice.

N-Acetyl beta-glucosaminidase [beta-2-acetamido-2-deoxy-D-glucoside acetylamido-deoxyglucohydrolase; EC 3.2.1.30] in the submandibular gland of mice was found to be androgen-dependent; the specific activities in males, females, and castrated males were 0.25, 0.11, and 0.11 unit/mg protein, respectively. The activities in females and castrated males were increased to the level of normal male mice by testosterone injection. Injections of progesterone and 17 beta-estradiol hardly affected the activity in males. In both males and females, the enzyme activity was detected in the convoluted tubular cells, not in acinous cells. The results of isoelectric focusing have shown that one enzyme having an isoelectric point of 9.0 is present in the glands of both sexes, indicating that the enzyme remains after castration and that the increases caused by testosterone represent the same molecular species. In addition, it was shown that the saliva from both sexes contained significant activity of N-acetyl beta-glucosaminidase, which also changed depending on the androgenic state of the animals. Most of the salivary activity was shown to originate from the submandibular gland, since the extirpation of this gland resulted in a significant decrease of the salivary activity.

Acetylglucosaminidase↗
PubMed1979

Induction of androgen-dependent protease and serous-like granules by tri-iodothyronine in the submandibular gland of mice with testicular feminization.

Esteroprotease, an androgen-dependent enzyme of the mouse submandibular gland, was increased by injection of tri-iodothyronine (T3) in mice with testicular feminization (Tfm) which are genetically deficient in androgen receptors. Histochemical and electron microscopic studies also demonstrated increases of RNA and serous-like granules in cells of the convoluted tubules of the gland. These findings suggest that the esteroprotease gene in Tfm mice is normal and that T3 can induced both esteroprotease and serous-like granules independently of androgen.

Androgen-Insensitivity Syndrome↗
PubMed1979

Regulation of delayed-hypersensitivity response by the submandibular gland of male mice.

This study was performed to determine whether the removal of submandibular gland (SG) of mice influences the delayed-hypersensitivity (DH) response. The removal of the SG increased the weights of thymus and spleen and enhanced DH response, only in males and not in females. The injection of crude extract of male mouse SG returned the enhanced DH response of SG-ectomized males to the normal level whereas the injections of crude extracts of female or castrated male mouse SG was not affected. These results suggested that SG of male mice contains an endocrine factor(s) to regulate DH response.

Animals↗
PubMed1978

Sex difference in L-glutamine D-fructose-6-phosphate aminotransferase activity of mouse submandibular gland.

L-Glutamine D-fructose-6-phosphate aminotransferase (2-amino-2-deoxy-D-glucose-6-phosphate ketol-isomerase (amino-transferring), EC 5.3.1.19) activities in the three main salivary glands of male and female mice were measured. It was found that the activity in the submandibular gland was about 10 times more in females than in males, whereas the activities in the sublingual and parotid glands of males and females were similar. The activity in the submandibular gland of female mice was not affected appreciably by ovariectomy but it decreased to the level in males on injection of testosterone. The activity in males was not affected appreciably by injection of progesterone or 17beta-estradiol, but it increased to the level in females after castration. The increased activity in castrated male mice was decreased again to the normal level by testosterone injection. Thus, this sex difference is caused by androgen, not by female hormones. On the basis of in vivo experiments using actinomycin D, it was suggested that testosterone produced an "enzyme inhibitor", which suppressed the enzyme activity in the submandibular glands of androgen-rich animals.

Animals↗
PubMed1978

Quantitation of berberine chloride in human urine by use of selected ion monitoring in the field desorption mode.

A method is described for the microdetermination of berberine chloride in human urine by a field desorption mass spectrometry selected ion monitoring system using a deuterium labelled analogue of berberine chloride as an internal standard. Prior to the quantitation of berberine in human urine, the fundamental problems related to field desorption selected ion monitoring, such as quality of emitters, amounts of sample loading, and the programming rate of the emitter current, were statistically investigated in detail. Berberine chloride can be determined in a concentration of 10 ng ml-1 in human urine by the method described. The analytical results were compared with those from gas chromatography mass spectrometry selected ion monitoring in the chemical ionization mode suggesting that the reliability of field desorption selected ion monitoring may be almost equivalent to that of gas chromatography chemical ionization selected ion monitoring.

Administration, Oral↗