Biomedical subjects

K Hrbková

Publications and source records attributed to K Hrbková.

9 recordsLinked to original sources

PubMed1998

Biochemical findings in the ejaculate of men with non-obstructive azoospermia.

In men with non-obstructive azoospermia, testicle biopsy can provide isolated sperm which can be used for fertilization of an oocyte. The male seminal plasma was examined for adequate biochemical parameters and then tested as potential diagnostic parameters for prediction of sperm presence in non-obstructive azoospermia. Sperm was obtained by preparation of the testicular tissue. Biochemical parameters in men with found sperm were compared to findings obtained from men without sperm in the testicular tissue. The following parameters were analysed: fructose, lactate dehydrogenase, total acid phosphatase, Zn, K, Na, Cl, pH. No statistically significant differences were found in both groups under study. Biochemical examination of the ejaculate cannot provide any prediction of sperm retrieval in non-obstructive azoospermia.

Humans↗

PubMed1998

Infertility treatment of men with non-obstructive azoospermia.

Male infertility can be treated by several methods with varying degree of success. We present evidence that "open" testicular biopsy is favorable for men suffering from non-obstructive azoospermia (NOA). Moreover, any NOA patient may be subjected to this treatment even though his past histopathological examinations suggest that it is likely no sperm will be found in the testicular tissue. Thus, we recommend the testicular sperm extraction (TESE) procedure for NOA patients.

Female↗

PubMed1998

[Treatment of male infertility after oncologic therapy: preventive autologous sperm preservation versus testicular biopsy and oocyte fertilization with testicular spermatozoa].

The prognosis of oncological therapy of some malignancies is favourable. Unfortunately the majority of young patients remain sterile. Sperm cryopreserved before treatment was successfully used in IVF-ICSI (in vitro fertilization-intracytoplasmic sperm injection) cycles. Testicular biopsy (TESE) was performed after successful oncological treatment in three men without previous sperm cryopreservation. No spermatozoa were found for oocyte fertilization. TESE could not ensure sperm recovery in all patients after oncological treatment. Sperm cryopreservation should be highly recommended to all patients before any treatment is offered.

Cryopreservation↗

PubMed1998

[Treatment of infertility in non-obstructive azoospermia using the TESE (testicular sperm extraction) method--clinical study].

Testicular sperm extraction (TESE) was performed in 27 men in 30 cycles. All men were examined for genetics, serum hormonal status, biochemical status of semen samples. All men were examined by an urologist. No prognostic evaluation able to provide information about the prognosis of TESE procedure was found. Even a high FSH level, testicular hypotrophy or previous histological examination cannot exclude any patient from testicular biopsy.

Adult↗

PubMed1998

[Treatment of infertility in nonobstructive azoospermia using the TESE method (testicular sperm extraction)--clinical results].

Sperm was found in 56.6% of TESE cycles in 27 men with non-obstructive azoospermia. Using testicular sperm, 30% oocytes were fertilized. Embryos were transferred in 11 cycles. Twins were delivered in the 34th week of pregnancy. Two further pregnancies are ongoing. The pregnancy rate was 27% per ET. Testicular biopsy in all azoospermic men is recommended in special IVF centers not only for histological examination but also for ICSI procedure with testicular sperm which could be provided at the same time.

Biopsy, Needle↗

PubMed1997

[The current role of intracytoplasmic single sperm injection (ICSI) in assisted reproduction].

Intracytoplasmic sperm injection (ICSI) plays a unique role in the treatment of male infertility. ICSI results are not influenced by either sperm number, motility or sperm morphology. In our group we studied 90 cycles in which conventional IVF and ICSI were performed on sibling oocytes in couples with borderline semen analysis (more than 50,000 sperm/oocyte). A higher number of A quality embryos was found in the ICSI oocyte group (40%/32%). In 38% of IVF cycles no embryos were found, whereas with ICSI the absence decreased to only 12%. In only 4% of the cycles, the IVF method was more successful than that of ICSI treatment. In only 7% of our combined group there were no embryos for transfer. The positive influence of ICSI on our centre results -32% pregnancy rate/ET and an 18% baby take home rate--are discussed. In our retrospective study, ICSI is more successful than IVF alone and a combination of IVF and ICSI achieves the best results in the borderline sperm analysis group. An increased use of the ICSI technique in assisted reproduction can be expected in future.

Female↗

PubMed1996

[The first pregnancy in the Czech Republic achieved by oocyte fertilization using ICSI--intracytoplasmic sperm injection].

ICSI--an intracytoplasmic sperm injection can fertilize an oocyte in vitro with one sperm, using micromanipulation. ICSI was done on an inverted Diaphot 300 microscope with Hoffmann modulation contrast and Narishige micromanipulators. Between March and May 1995 23 ICSI cycles were performed. Of 166 oocytes (7.2 per cycle) 22% were degenerated or immature and 6% damaged during ICSI. 45% of the injected eggs were fertilized. Embryos were transferred in 65% couples. Three pregnancies resulted (20%/ET), all are viable. ICSI is suitable in cases of severe oligospermia, asthenospermia and teratospermia, immunology infertility and low cleavage rate of oocytes in the first IVF cycle.

Adult↗

PubMed1996

[Cryopreservation of embryos with the Cryogenetic apparatus].

For cryopreservation of human supernumerary embryos in our IVF programme we use a "Cryogenetic" computerised freezer. 1,2-propandiol and sucrose were used as cryoprotectant in the slow freezing/thawing protocol. In 20 transferred cycles we achieved 3 pregnancies. One is proceeds. Although the number of described cycles is low, the results are comparable with other centres. Our centre is the second one in the Czech Republic, which published data on a pregnancy after the transfer of cryopreserved embryos. Our Cryogenetic freezer is a high quality freezer suitable for gamete and embryo freezing.

Cryopreservation↗

PubMed1996

[Evaluation of sperm morphology using the "strict" method].

The asset of evaluation of the morphology of sperm cells for prediction of their fertilizing power is generally accepted. Advances in the sphere of extracorporeal fertilization and the onset of micromanipulation techniques (intracytoplasmic injection of a single sperm cell - IC-SI) called for a stricter evaluation which is the prerequisite for a high standard indication and selection of different methods of extracorporeal fertilization. We obtained the consent of Prof. Gruger and Prof. Menkveld from the Tygerberg University (South African Republic) who elaborated this evaluation, to publish their criteria in our journal.

Humans↗