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Biomedical subjects

K Huse

Publications and source records attributed to K Huse.

33 records · Page 2Linked to original sources
PubMed1982

A new procedure for the purification of proteinase B from baker's yeast and interaction of the purified enzyme with a specific inhibitor.

Proteinase B from baker's yeast was purified to homogeneity by applying affinity chromatography with D,L-tyrosine ethyl ester as ligand. The molecular parameters of the product are similar to those reported formerly by other groups. A different form of proteinase B is isolated if affinity chromatography is replaced by CM-cellulose chromatography and gel filtration. In this case a peptide tightly associated with the enzyme was found to occur. This could be identified as an inhibitor fragment produced by limited proteolysis of a proteinase B bound inhibitor induced by proteinase A.

Chromatography, Affinity↗
PubMed1980

[Clinical experiences with diazepam (Valium) - fentanyl combination anesthesia in neurosurgery. A study of circulation and oxygen consumption].

This clinical study was performed on 13 neurosurgical patients. The mean age was 56 years (range 45-70 years). After induction of anesthesia the heart rate diminished by 21.4%, the mean pressure by 12.2% in the same time decreased the cardiac output by 19.3%, the calculated total peripheral resistance increased by 13%. The oxygen uptake decreased by 16%. The advantages and contraindications of this technique in neuroanesthesia are discussed.

Aged↗
PubMed1980

[Circulatory effects of dihydrazinophthalazine (Nepresol) compared with sodium nitroprusside in controlled hypotension. A study of neurosurgical patients under neuroleptanesthesia].

In 32 neurosurgical patients in neuroleptanaesthesia and controlled hypotension the following hemodynamic parameters were studied. Blood pressure, heart rate, cardiac output in addition serial measurements of the blood gasanalysis, oxygen content were performed from the arterial and centralvenous blood samples. In the first collective of 12 patients a mean dosis of 45 +/- 12 mg dihydrazinophthalazine (Nepresol) decreased the blood pressure to a mean value of 55 +/- 7 mm Hg. In spite of this blood pressure drop the cardiac output increased by 62,4% in consequence to the increase of the heart rate by 27% and the stroke volume by 19,9%. A controlled hypotension to a mean value of 57 +/- 9 mm Hg was performed in 20 patients with sodiumnitroprusside. In contrast to the effects of Nepresol after medication of sodiumnitroprusside the cardiac output decreased by 16% the stroke index by 24% while the heart rate increased by 18%. Both drugs have a useful place in neuroanesthesia, but sodiumnitroprusside is the drug of choice for controlled hypotension, because after medication of Nepresol even in the higher dose range there is no dose-response-relationship and the onset of action is delayed.

Adult↗
PubMed1980

[Circulatory changes in neurosurgical patients with intracerebral angiomas in neuroleptanaesthesia (author's transl)].

In 10 neurosurgical patients with intracerebral angiomas circulatory and respiratory functions were studied. After induction of anesthesia the cardiac output declined from an average value of 7.63 +/- 2.13 1.min-1 to 3.99 +/- 0.43 1.min-1 (-48%) in consequence to the decline of the heart rate by 24% and the stroke volume by 32%. In the same time increased the calculated peripheral resistance in reverse proportion to the decline of the cardiac output by 47%. The oxygen transport decreased after induction of anaesthesia by 48% while the oxygen uptake decreased by 28.6%. The oxygen utilization coefficient increased by 48% (0.33) and documented a satisfactory relation between oxygen-uptake and oxygen utilization in neuroleptanaesthesia.

Adolescent↗
PubMed1979

[Circulatory changes in the sitting position during neurosurgical anaesthesia (NLA) (author's transl)].

In 23 neurosurgical patients with haemodynamic effects of neuroleptanaesthesia in the supine (13 patients) and upright position (10 patients) were studied. The patients in the upright position demonstrated an increase of the mean arterial pressure of 19% and the total peripheral resistance of 45%, and simultaneously a decreased stroke index of 20% and the cardiac index of 16%. The stability of the circulation was preserved due to the increase of heart rate (to 90 +/- 17 beats/min) and total peripheral resistance. In spite of the almost identical average total dose of fentanyl in both groups (4.72 +/- 2.2 mcg/kg/h), supine position) and 4.97 +/- 2.23 mcg/kg/h, (sitting position), only the patient group in the supine position demonstrated a decrease of pulse rate of 36% to 65 +/- 20 beats/min with a decline of the cardiac index of 33% and unchanged stroke index. Our clinical observations and the animal studies of other authors allow the conclusion that during neuroleptanaesthesia in the upright position the baroreceptor-activity and the catecholamine secretion is not markedly reduced and consequently circulatory stability obtained. We regard neuroleptanaesthesia as the method of choice for neurosurgical operations in the upright position.

Adolescent↗
PubMed1978

[The effect of buprenorphin on circulation, respiration and the electroencephalogram (a postoperative study of 12 neurosurgical cases) (author's transl)].

Buprenorphin was given to 12 patients as a postoperative analgesic after neurosurgery, and the effects of the drug on the circulation, respiration and the electroencephalogram were studied. The circulation was not significantly affected, respiration was moderately depressed, the analgesic action was satisfactory. The depression of consciousness was reflected in the electroencephalographic pattern.

Adolescent↗
PubMed1976

[Cardiovascular changes caused by atropine in normo- and hypothermic methoxyflurane anaesthesia (author's transl)].

The authors examined in 25 patients, of which 15 were normo- and 10 in hypothermic, the cardiovascular effects of 1 mg atropine i.v. In normothermia the heart rate increased significantly from 100 to 110 beats/min after atropine. At the same time the stroke index and stroke work decreased significantly. The mean arterial pressure, the heart index, the left ventricular minute- and stroke work and the total peripheral resistance did not change. In two patients with an arteriovenous fistula and hypervolaemia, the atropine injection caused an increased of heart- and stroke index. Arrhythmias did not occur after atropine. In hypothermia on the other hand atropine was shown to have no effect on heart frequency and all other examined parameters. In one patient in which the P-R interval was shortened, the atropine injection was followed by an total atrio-ventricular block. The authors cannot recommend atropine therapy in cases of hypothermic bradycardia, because of its lack of effect on the heart rate in hypothermia.

Adult↗