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Biomedical subjects

K Husek

Publications and source records attributed to K Husek.

At least 19 recordsLinked to original sources
PubMed2003

Epithelioid hemangioendothelioma of the mandible.

The authors describe a locally recurrent tumor of the mandible whose microscopic structure and immunohistochemical findings corresponded to an epithelioid hemangioendothelioma. The tumorous cells had an epithelioid character, they created rudimentary vascular lumina, and they focally expressed vascular markers CD 34, CD 31, and F VIII. Locally the tumor grew aggressively. During the last recurrence, the tumor even affected the already healed and rebuilt bone graft, which was incorporated after the primary resection of the mandibular body. From the histological point of view, the tumor approximated an angiosarcoma. No metastases occurred.

Aged↗
PubMed2003

[Fibrillary immunotactoid glomerulonephritis--an unusual cause of irreversible kidney failure].

Fibrillary-immunotactoid glomerulopathy (FITGP) is a rare cause of nephrotic syndrome. In this patient, male 65-years-old, respectively, fibrillary glomerulonefritis (FGN) was diagnosed by percutaneous renal biopsy. Clinically, the disease manifested with long term proteinuria and nephrotic syndrome without any associated systemic disorder. Histologically, glomeruli showed deposition of PAS+, fuchsinophilic and Orange G+ material in the mesangium and basal membranes. Strong granular immunofluorescent IgG, C3, kappa and lambda light chains deposition was present in the mesangium. Electronmicroscopically, depositions of fibrillary material in the expanded mesangium and in the peripheral basal membranes were found. Randomly distributed nonbranching fibrils measured 18-28 nm. After 18 months of follow-up, the therapy with corticosteroids and Cylosporin A was without effect, and the disease progressed into chronic renal failure, and after 24 months of biopsy the patient is undergoing hemodialysis now.

Aged↗
PubMed2002

[Lymphoproliferative disease after transplantation].

Herein we describe 7 cases of posttransplantation lymphoproliferative disease (PTLD), 5 in men and 2 in women (aged from 25 to 62 years), occurring from 4 months to 12 years (mean, 7 years) after transplantation. Our patients were recipients of kidney, kidney and pancreas, heart, and autologous peripheral haematopoetic stem cells. Four cases were diagnosed as monomorphic and three as polymorphic type of PTLD according to the WHO classification. Monoclonal immunoglobuline heavy chain gene rearrangement was detected in two monomorphic lesions and one polymorphic lesion by polymerase chain reaction (PCR). In the two cases of polymorphic and the one case of monomorphic PTLD, the presence of EBV was visualised by immunohistochemical staining of some transformed lymphoid cells for latent membrane protein (LMP) of EBV. The presence of type A EBV was demonstrated by PCR. The patients were treated by reduction or discontinuation of immunosuppression and by chemotherapy. In 2 cases, a part of the organ affected by lymphoma (sigmoid colon and pancreas) was surgically resected. Four patients died of causes related to PTLD (2 to 15 months after the diagnosis), mainly of infectious complications. Two other patients who achieved remission died of unrelated causes. Only the youngest man is alive and in the complete remission 10 months after the diagnosis of PTLD.

Adult↗
PubMed2002

Extraorbital giant cell angiofibroma.

We describe a case of giant cell angiofibroma (GCA) in extraorbital location. A 47-year-old man developed a subcutaneous tumor on the forearm, which measured 6 cm in diameter and was well demarcated. Histologically, small-sized vessels similar to arterioles, sometimes with a hyalinized wall, were regularly distributed throughout the tumor tissue. Lesional cells were oval and spindle in shape with pale eosinophilic cytoplasm and plump nuclei with clumped chromatin. In addition, larger bizarre cells with lobulated hyperchromatic nucleus were randomly admixed. Areas with a sieve-like pattern contained small slit-like spaces or larger pseudocystic spaces lined by tumor cells with an admixture of bizarre cells. Both mononuclear and bizarre lesional cells expressed strongly and diffusely vimentin and CD34. Negative reactions were with cytokeratins, bcl-2, desmin, actin, VWF, S100, alpha-1-antitrypsin, CD99, lysozyme and FXIIIa. Our finding confirms that GCA is a benign neoplasm not necessarily confined to orbital location. The patient did well without recurrence after 13 months of follow-up.

Angiofibroma↗
PubMed2002

[Fibrillary glomerulonephritis].

Fibrillary glomerulonephritis (FGN) is a rare cause of nephrotic syndrome. In three patients (65-year-old male; 57- and 38-year-old females) FGN was diagnosed by percutaneous renal biopsy. Clinically, the disease manifested itself with long term proteinuria and nephrotic syndrome without any associated systemic disorder. Histologically, glomeruli showed deposition of PAS+, fuchsinophilic and Orange G+ material in the mesangium and basal membranes. Strong granular immunofluorescence IgG, C3 and weak kappa and lambda light chains mesangial and peripheral deposition were observed in two patients. Electronmicroscopically, deposition of fibrillary material into mesangium and basal membranes was found. Randomly distributed nonbranching fibrils were 18-28 nm thick. After 10 to 13 months of follow-up, the therapy with corticosteroids and immunosuppressives was without effect, and the disease progressed into chronic renal failure in two patients; the third patient did well.

Aged↗
PubMed2001

[Sclerosing epithelioid fibrosarcoma].

Sclerosing epithelioid fibrosarcoma (SEF) was first described in 1995 and since then 39 cases have been reported. Here we describe 6 cases of SEF (3 in women and 3 in men). The patients aged from 22 to 79 years. The tumours were located in soft tissues of the extremities (in 3 cases in the lower, in 2 instances in the upper extremity) and of the trunk (in 1 case). The lesions were partially nodular, of gray-white colour, and hard in consistency. Histologically, they were composed of epithelioid round to ovoid small cells with a sparse cytoplasm and a very low mitotic activity. The tumour cells formed cords and alveoli or were scattered individually within a dense hyalinized collagenous stroma. The neoplasms also contained foci of conventional fibrosarcoma, necrosis, calcification, and metaplastic bone. On immunohistochemistry, the neoplastic cells were positive for vimentin. Two cases were immunoreactive for epithelial membrane antigen and one tumour also for cytokeratins. The proliferative activity, assessed by MIB 1 antibody (Ki-67), was detected in 1-6% of neoplastic cells in primary tumours. Follow-up information was available in 5 patients. In two cases, there were local recurrences and distant metastases (in the lungs, upper extremity, and mediastinum). One of these patients died of SEF. The differential diagnosis of this relatively low-grade fibrosarcoma is broad and includes, along with a variety of benign and malignant soft tissue lesions, infiltrating carcinoma, and, to a lesser extent, sclerosing lymphoma.

Adult↗
PubMed2001

Pleomorphic hyalinizing angiectatic tumor.

Authors present an additional case report of pleomorphic hyalinizing angiectatic tumor (PHAT), only recently recognized entity amongst the soft tissue tumors. 63-year-old man underwent surgery for subcutaneous tumor growing on the forearm. Grossly, ovoid well demarcated tumor 4.0 x 3.5 x 2.5 cm was removed. Histologically, hypovascular areas of spindle-cells arranged in sheets and fascicles resembling spindle-cell sarcoma and areas rich on ectatic vessels with fibrin deposits or thickened hyalinized walls were present. Some cells contained intranuclear cytoplasmatic inclusions, lobulated nuclei with or without prominent nucleoli or multiple nuclei. Mitoses were less than 1 per 30 HPF, Ki-67 nuclear positivity was observed in 2.6% of tumor cells. Immunohistochemically, cells were positive with vimentin, but negative for CD34, S100, desmin, smooth-muscle actin, EMA, VWF, CD99, bcl-2 and cytokeratins. The patient did well, without recurrent tumor after 13 months follow-up. Spectrum of tumors including solitary fibrous tumor, giant-cell angiofibroma, neurilemmoma, malignant fibrous histiocytoma and acral myxoinflammatory tumor with atypical bizarre giant cells is discussed in differential diagnosis.

Diagnosis, Differential↗
PubMed2001

[Incidence of carcinomas in goiters and their combination with Hashimoto's thyroiditis].

In the investigated group 300 patients were operated for a thyropathy mostly struma nodosa, tumours were proved in 69 cases; 38 of them were carcinomas of various extent and 3 of them were combined with Hashinoto's lymphomatous goitre. The overwhelming majority of the carcinomas were papillary carcinomas (22 cases). The incidence of papillary carcinoma in Hashimoto's goitre was about 8%; this fact calls for more attention to autoaggressive thyroiditis both in examination of surgical material, and during autopsies in middle-aged women, where this thyroiditis is often clinically silent. Our results suggest an increase in the incidence of carcinoma in Hashimoto's goitre and the generally accepted rarity of this coincidence is questioned. We recorded also a lower incidence of medullary carcinoma than the worldwide average and a more frequent incidence of mixed-type carcinoma.

Adenoma↗
PubMed2000

[Diagnosis of malacoplakia in a transplanted kidney by needle biopsy].

Malakoplakia is an uncommon inflammatory condition rarely involving parenchyma of transplanted kidney. We report a 44-year-old female recipient of a cadaveric renal allograft treated with cyclosporin A and prednisone. After transplantation, E. coli and Citrobacter bacteruria occurred and three years later decreased graft function developed. Percutaneous needle biopsy was performed and diagnosis of malakoplakia was established. Histologically, interstitial sheets of plasmocytes and histiocytes with periodic acid-Shiff positive cytoplasm containing Michaelis-Gutmann bodies were present. Ultrastructurally, phagolysosomes containing membrane fragments and various developmental stages of inclusions to fully developed Michaelis-Gutmann bodies were found. The patient was treated with co-piperacillin and subsequently pefloxacin and renal functions improved after six months follow-up. Our case suggests that malakoplakia represents an abnormal defective histiocytic response to the infection in the setting of immunosuppressive therapy.

Biopsy, Needle↗
PubMed1998

[Effects of carvedilol, a sympatholytic, in experimental alloxan diabetes in laboratory rats].

A possible effect of the sympatholytic carvedilol on alloxan-induced diabetes mellitus in the laboratory rat was examined in experiments. The animals were divided into a group treated with carvedilol in a single daily dose of 10mg/kg in 1 ml of diluting solution i.p and the control group which received only diluting solution in the pertinent amount. The values of malondialdehyde and glucose in the serum, diuresis and total losses of sugar in the urine within 24 hours were estimated and histopathological examination of the kidneys of the treated and control groups was performed. The results show an effect of the tested dose of the drug, primarily in the region the proximal renal tubule.

Adrenergic Antagonists↗
PubMed1998

[Low grade malignant fibromyxoid sarcoma].

Two cases of low-grade fibromyxoid sarcoma are presented. In a 31-year-old male the tumour arouse in the scapular soft tissues and local recurrence occurred. In a female of 45, the neoplasm was located in the thigh. Histologically, the neoplasms with low cellularity of deceptively benign-appearing small fibroblastic spindle cells demonstrated alternating dense fibrous and loose myxoid areas, showing a mainly whorled and swirling growth pattern. In myxoid areas a prominent vascular component was present. Mitoses and cellular atypia were absent. Tumor cells showed staining with anti-vimentin and occasionally anti-actin antibodies. As a distinctive soft-tissue sarcoma, low-grade fibromyxoid sarcoma had to be distinguished from variety of benign and malignant soft tissue tumours such as neurofibroma, liposarcoma, myxoid MFH and others.

Adult↗
PubMed1997

[Aggressive angiomyxoma of the pelvic-peritoneal region (case report)].

The authors describe the case-history of a 30-year-old woman with an aggressive angiomyxoma. This rare tumour is usually found in the pelvic and perineal area and reaches a big size. In the presented case the weight of the tumour was 1020 g. The most successful therapeutic method is radical surgical extirpation of the tumourous mass. Inadequate removal is the cause of frequent relapses. The paper contains also a review of the literature.

Adult↗
PubMed1997

[The effects of Carvedilol, a beta-blocker, in experimental ischemia-reperfusion kidney injury].

Carvedilol is a recently introduced drug with multiple action with a non-selective beta-antiadrenergic and selective alpha1-antiadrenergic action used for treatment of mild to medium severe hypertension. The authors investigated in their experiments the protective effect of carvedilol under conditions of ischaemia-reperfusion of the kidney in the laboratory rat. The animals were divided into four groups 1. the control group was fed a diet without carvedilol for a period of two weeks. Groups 2, 3 and 4 were fed for two weeks a diet containing carvedilol, 1-3-10 mg/kg/day resp. After completed medication in all animals ischaemia of the kidney was induced (60 min.) with subsequent reperfusion (10 min.) Then the animals were sacrificed, the kidney was removed for histopathological examination, in blood the malondialdehyde (MDA) level was assessed. The conclusions of the investigation indicate a marked protective effect of the administered preparation. Carvedilol prevents the disintegration of tubular epithelia, pycnosis of the nuclei, and reduced the development of oedematous changes. These findings correlate with MDA levels.

Adrenergic beta-Antagonists↗
PubMed1994

[Protective effect of carvedilol on renal circulation and tissue injury in reperfusion of ischemic kidneys in rats].

In one-kidney rats the resting blood flow in the renal artery was recorded for 10 minutes then the artery was clipped for one hour and the following reperfusion blood flow was registered again for 10 minutes. The animals were divided into three groups: Series A, untreated control animals. Series B, two weeks before renal ischemia the rats were fed a diet containing 0.1% of BM-14.190, a derivate of carvedilol. Series C the rats received a diet with 0.1% of carvedilol. The resting renal blood flow was 4.58 +/- 0.13 ml/min (x +/- S.E.M.) in series A whereas 6.36 +/- 0.41 ml/min. in series B and 5.87 +/- 0.59 ml/min. in series C (p < 0.01), also the reperfusion blood flow in series B and C was significantly higher (p < 0.01) i.e. 3.65 +/- 0.25 ml/min. and 3.08 +/- 0.33 ml/min. respectively than in series A, 1.08 +/- 0.13 ml/min. The ischemia and the ensuing reperfusion elicited in series A serious injury, especially in renal proximal tubules and their reticulin skeleton whereas the renal injury in series B and C was of a markedly lesser degree. Carvedilol and its derivate BM-14.190 possess beside vasodilatory potency and alpha- and beta-adrenoceptor blocking activity a potent scavenger property. This may be the cause of the protective effect preventing the ischemia and reperfusion injury in the renal tissue.

Animals↗
PubMed1993

Aggressive angiomyxoma of the pelvioperineal region. Immunohistological and ultrastructural study of seven cases.

We describe seven cases of aggressive angiomyxoma of the pelvioperineal region. In three cases, we observed immunohistochemical signs of myoid differentiation in the tumor cells. In one of these cases, we could confirm the myoid differentiation at the ultrastructural level. The other four cases were devoid of this differentiation. We further discuss the relationship of aggressive angiomyxoma to the recently described angiomyofibroblastoma of the vulva.

Actins↗
PubMed1992

[Malignant transformation in multiple eccrine spiradenoma].

Development of a poorly differentiated eccrine carcinoma was observed in a 6-year-old woman. She had been operated on many times during 20 years for some tens of classical as well as less usual forms of eccrine spiradenomas, e.g. giant vascular spiradenomas. They were mostly localized in the skin of back, thorax and neck. The patient died of an extensive skin involvement and spine and liver secondaries 12 months after occurrence of the carcinoma. The structure of carcinoma was trabecular and partly papillary, tumour cells had bulky nuclei and striking nucleoli. There was a juxtaposition of spiradenomas with carcinoma and direct transformation of spiradenoma into carcinoma was observed. Immunohistological positivity of carcinoma concerned S-100 protein, slightly CEA, focally cytokeratin 7 and 18; cytokeratin 14 was negative. Ultrastructure of tumour cells showed irregular intercellular lumina with some microvilli, but structures characteristical for eccrine glandular of ductal differentiation were lacking.

Adenoma, Sweat Gland↗
PubMed1992

[Liver diseases in children with alpha 1-antitrypsin deficiency in infancy].

The authors evaluate the health status of children with alpha-1-antitrypsin deficiency, focused on liver disease in infant age. The children were selected by neonatal screening. Of 21 children one had severe neonatal hepatitis with progression to cirrhosis, 2 children had clinically apparent jaundice to the age of two months, 6 children had elevated total bilirubin and transaminase levels without clinical signs of the disease, 12 of the remaining children had no clinical and laboratory signs of liver disease. In the discussion the authors compare the results with data published abroad.

Female↗