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Biomedical subjects

K Inada

Publications and source records attributed to K Inada.

At least 271 records · Page 15Linked to original sources
PubMed1982

Studies of human tear proteins--1. Analysis of tears from normal subjects by crossed immunoelectrophoresis.

Tears were collected from 10 normal subjects by two methods, 1) micropipetting after stimulation with an onion slice, and 2) Schirmer 1 test using a filter paper strip. The tears in the 5 x 5 mm part of the filter paper placed within the conjunctival sac were extracted with a 0.9% NaCl solution. The tear samples so obtained were analyzed by crossed immunoelectrophoresis. Nineteen proteins were identified by the use of monospecific antisera. Ten tear-specific proteins including the specific tear prealbumin of Bonavida et al., lactoferrin and lysozyme were identified by the intermediate gel method. Some differences were found in the pattern of the tear-specific proteins between the tears collected by the two methods, but the pattern was basically similar. Some individual differences were also found. The levels of serum proteins including albumin and IgG varied considerably, but it was thought to be due to dilution by reflex lacrimation during tear sampling. On the other hand, the pattern of the tear-specific proteins was fairly constant. The filter paper method allowed tear protein analysis, even in patients with 0 mm wetting by the Schirmer 1 test. A study of the length-volume relationship of the filter paper wetting showed that the tears contained in the 5 x 5 mm portion of the filter paper were about 1.8 microliters. The filter paper method was thought to offer a reliable clinical tool for the study of the tear proteins in various pathological conditions of the lacrimal secretion.

Adult↗
PubMed1980

Complement activating property of the protein-rich endotoxin (OEP) of Pseudomonas aeruginosa. II. Complement activating property of the lipopolysaccharide portion and the inhibition by polymyxin B.

In order to elucidate the active principle and the mechanism of complement activation of protein-rich endotoxin (OEP) of P. aeruginosa, the complement consumption of the LPS from OEP was studied either in guinea pig serum (GPS) or in factor D-depleted GPS (D-dpl-GPS) in the presence or absence of polymyxin B. Polymyxin B is an inhibitor of the classical pathway (CP) activation due to the lipid A of LPS. The LPS from OEP had about 5 times higher the activity than OEP in GPS. This value corresponds to the LPS content (about 20%) in OEP. It was not likely that the protein portion (about 80%) of the OEP participates to activate complement. In GPS, the ability of the LPS from OEP was only partially inhibited by polymyxin B. In D-dpl-GPS in which the alternative pathway (AP) may be abrogated, the consumption by the LPS from OEP markedly decreased, and was furthermore diminished in the presence of polymyxin B. These results suggest that the LPS from OEP is the active principle of OEP and activates both the CP and the AP. It was noteworthy that two fold or less polymyxin B in weights did not inhibit the ability of E. coli 0111:B4 LPS to activate only the AP, but rather enhanced it.

Animals↗
PubMed1980

Complement activating property of the protein-rich endotoxin (OEP) of Pseudomonas aeruginosa. I. Activation of both the classical and the alternative pathways of guinea pig complement.

Complement activating property of the protein-rich endotoxin (OEP) of Pseudomonas aeruginosa was investigated. The ability of OEP to consume the hemolytic complement (CH50) of guinea pig serum (GPS) was relatively lower than zymosan, a potent activator of the alternative pathway (AP). The profiles of the complement consumption with OEP in GPS suggested that classical pathway (CP) activation seems to occur in addition to the AP-activation, because the consumption of the C3-C9, i.e., C3 total was relatively higher than those of C1, C4 and C2. Further results stated below is confirmed this. OEP consumed complement in certain extent in C4-deficient GPS and also in factor B- or D-depleted GPS. In these sera either pathway of the AP or the CP was operated. However, OEP consumed complement quite in full extent in the EGTA-chelated serum, in which AP-activation took place in normal level. OEP was therefore found in this experiment to activate only the AP. The conversion of C3 in immunoelectrophoresis which is a evidence of the CP-and/or AP-activation was observed with OEP. It was suggested that the antibody against OEP did not participate in the consumption of complement, because the GPS priorly absorbed with OEP-coated sheep RBC, was retained fully to react with OEP. It was discussed that the active site of OEP as to the complement activation located on the LPS portion of OEP.

Animals↗
PubMed1978

Popliteal artery entrapment syndrome: a case report.

A rare case of bilateral popliteal artery entrapment syndrome is reported. Pathological changes of the occluded lesion in this anomaly were investigated in 8 other cases. Abnormal longitudinal muscle in the media was noted in all. Development of these muscle bundles was considered to be due to chronic trauma inflicted by pressure on the arterial wall. Its significance is emphasized to assist in differentiation from other chronic arterial occlusive disease.

Adolescent↗
PubMed1978

Surgical treatment of thoracic aneurysm in a hemodialysis-dependent patient: a case report.

A 53-year-old woman who was on hemodialysis underwent the resection of an aneurysm of the descending thoracic aorta as well as graft replacement. The operation was performed with the aid of femoro-femoral bypass perfusion and an artificial kidney inserted on the venous side of bypass line. Although the perfusion time was short, the uremic metabolic substance was efficiently removed, resulting in easy management after surgery.

Aorta, Thoracic↗