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K Inada

Publications and source records attributed to K Inada.

At least 73 records · Page 4Linked to original sources
PubMed1984

Studies of human tear proteins. 3. Distribution of specific tear prealbumin in lacrimal glands and other ocular adnexa.

Tear fluid is a mixed solution containing many kinds of proteins, and two predominant fractions have been determined by electrophoretic studies. One is anodal specific tear prealbumin (STP) and the other is cathodal lysozyme. This study is the first to demonstrate the localization of STP in the ocular adnexa by the immunofluorescence method. STP was found in the acinar cells of both the main and the accessory lacrimal glands. The cells of the interlobular ducts showed no or only weak staining of STP. No specific staining of STP was found in the conjunctiva, cornea or Meibomian gland. STP as well as lysozyme are thought to be secreted from the main and the accessory lacrimal glands.

Aged↗
PubMed1984

Quantitative determination of human aqueous proteins by crossed immunoelectrophoresis.

Crossed immunoelectrophoresis having high resolving power and sensitivity was applied to the human aqueous humor, in an attempt to determine which of the following is the most appropriate parameter for detecting the concentration of protein components: 1) the height of the precipitation line, 2) the length of the line itself or 3) the area under the precipitation line. Furthermore, the concentrations of several proteins were determined in the aqueous humor from normal subjects. Although it has been reported that the area under the precipitation line was proportional to the concentration of the protein, the present study showed that both the height and the length of the precipitation line are also equally valid for concentration determinations. The amount of protein antigens in 100 ml of the normal human aqueous humor were prealbumin 0.31-0.41 mg, albumin 5.4-6.5 mg, alpha 1-acid glycoprotein 0.05-0.17 mg and transferrin 1.3-1.7 mg.

Adult↗
PubMed1983

Postoperative deep venous thrombosis in Japan. Incidence and prophylaxis.

The incidence of postoperative deep venous thrombosis was investigated using the iodine-125-fibrinogen method in 256 patients undergoing major surgery. Deep venous thrombosis was found in 49 patients (15.3 percent), and nonfatal pulmonary embolism developed in one of seven patients in whom the thrombus extended to the popliteal vein. The same investigation was performed in 110 patients who wore a graduated compression stocking on one leg, with the other leg serving as a control. Deep venous thrombosis was found in 4 of 110 stockinged legs (3.6 percent) and in 16 of 110 control legs (14.5 percent). The incidence of deep venous thrombosis decreased significantly in patients who wore the stocking. An increase in femoral venous flow velocity was found in the stockinged legs by the Doppler method. The mean velocity of venous return by xenon-133 clearance was significantly greater in the stockinged legs than in the control legs. These findings were considered to support the efficacy of graduated compression stockings for the prevention of deep venous thrombosis.

Adolescent↗
PubMed1983

Diabetic gangrene in Japan: analysis of 487 cases.

Diabetic gangrene was thought of as a rather rare complication in Japan. Since the first report in 1917, only 16 cases were reported up to 1945. However, the number of reports increased rapidly in the manner of a geometric series after 1965. Sex and age distributions of the syndrome in Japan corresponded closely with those of Western countries. A distinct tendency in the inducement of gangrene was found, i.e. thermal trauma had preceded 43.6% of the cases. This might be the result of the local habit of warming the feet using hot-water bottles or foot warmers. The number of patients with normal pulses in the affected extremities in Japan was distinctly higher compared with that reported in Western countries. But functional and morphological changes in large peripheral vessels were frequently demonstrated when more detailed examinations were conducted. The cause for the rapid increase in the incidence of diabetic gangrene has not been elucidated, but changes in lifestyle may be partly responsible. The most conspicuous change was the increase in meat and fat intake during the 1960's. The changes in total calorie, total protein or sugar intake over this period were inconspicuous. Changes in dietary habits seem to be responsible, but further research will be necessary to clarify the details.

Aged↗
PubMed1983

Studies of human tear proteins--2. Analysis by crossed immunoelectrophoresis of tears from diseased eyes.

By filter paper strips, tears were collected from patients with diseases in the anterior ocular segment and analysed by crossed immunoelectrophoresis. Their crossed immunoelectrophoretic patterns were compared with those of normal subjects. Tear proteins from Sjögren's syndrome. Mikulicz's disease and tumor of the lacrimal gland showed abnormal patterns by crossed immunoelectrophoresis. Tears were collected from patients with lacrimal gland tumor after resection of the tumors. Ten tear-specific proteins were more decreased in these patients than in normal subjects. Serum proteins in the tears of these patients were increased, but secretory IgA (s-IgA) showed no change. A patient with symblepharon showed a marked decrease of both tear-specific proteins and s-IgA. Tears from patients wit epidemic keratoconjunctivitis (EKC), vernal conjunctivitis, herpes simplex keratitis (HSK) and adult inclusion conjunctivitis were analyzed. No remarkable change in tear-specific proteins could be noticed between tears from a case with an early stage of EKC or slight inflammation of the conjunctivitis and tears from the fellow unaffected eye. However, an increase was noted in serum proteins of the diseased eye. The tears collected from a case with severe EKC showed a decrease of tear-specific proteins, but an increase of serum proteins; especially s-IgA showed a significant increase. Tears from patients with EKC, HSK, vernal conjunctivitis and adult inclusion conjunctivitis showed differences in the immunoelectrophoretic patterns of tear proteins according to the severity and duration of the inflammation, as well as the amount of tears secreted. After due consideration of the analysis of tear proteins from some diseases of the anterior ocular segment, the following secretory sites of tear proteins under normal conditions were projected. The 10 tear-specific proteins reported in the previous paper are thought to be largely secreted from the main lacrimal gland. s-IgA is mainly secreted through the excretory ducts of the main lacrimal gland. Serum proteins in the tears are thought to come through the conjunctiva.

Adolescent↗
PubMed1982

Studies of human tear proteins--1. Analysis of tears from normal subjects by crossed immunoelectrophoresis.

Tears were collected from 10 normal subjects by two methods, 1) micropipetting after stimulation with an onion slice, and 2) Schirmer 1 test using a filter paper strip. The tears in the 5 x 5 mm part of the filter paper placed within the conjunctival sac were extracted with a 0.9% NaCl solution. The tear samples so obtained were analyzed by crossed immunoelectrophoresis. Nineteen proteins were identified by the use of monospecific antisera. Ten tear-specific proteins including the specific tear prealbumin of Bonavida et al., lactoferrin and lysozyme were identified by the intermediate gel method. Some differences were found in the pattern of the tear-specific proteins between the tears collected by the two methods, but the pattern was basically similar. Some individual differences were also found. The levels of serum proteins including albumin and IgG varied considerably, but it was thought to be due to dilution by reflex lacrimation during tear sampling. On the other hand, the pattern of the tear-specific proteins was fairly constant. The filter paper method allowed tear protein analysis, even in patients with 0 mm wetting by the Schirmer 1 test. A study of the length-volume relationship of the filter paper wetting showed that the tears contained in the 5 x 5 mm portion of the filter paper were about 1.8 microliters. The filter paper method was thought to offer a reliable clinical tool for the study of the tear proteins in various pathological conditions of the lacrimal secretion.

Adult↗
PubMed1980

Complement activating property of the protein-rich endotoxin (OEP) of Pseudomonas aeruginosa. II. Complement activating property of the lipopolysaccharide portion and the inhibition by polymyxin B.

In order to elucidate the active principle and the mechanism of complement activation of protein-rich endotoxin (OEP) of P. aeruginosa, the complement consumption of the LPS from OEP was studied either in guinea pig serum (GPS) or in factor D-depleted GPS (D-dpl-GPS) in the presence or absence of polymyxin B. Polymyxin B is an inhibitor of the classical pathway (CP) activation due to the lipid A of LPS. The LPS from OEP had about 5 times higher the activity than OEP in GPS. This value corresponds to the LPS content (about 20%) in OEP. It was not likely that the protein portion (about 80%) of the OEP participates to activate complement. In GPS, the ability of the LPS from OEP was only partially inhibited by polymyxin B. In D-dpl-GPS in which the alternative pathway (AP) may be abrogated, the consumption by the LPS from OEP markedly decreased, and was furthermore diminished in the presence of polymyxin B. These results suggest that the LPS from OEP is the active principle of OEP and activates both the CP and the AP. It was noteworthy that two fold or less polymyxin B in weights did not inhibit the ability of E. coli 0111:B4 LPS to activate only the AP, but rather enhanced it.

Animals↗
PubMed1980

Complement activating property of the protein-rich endotoxin (OEP) of Pseudomonas aeruginosa. I. Activation of both the classical and the alternative pathways of guinea pig complement.

Complement activating property of the protein-rich endotoxin (OEP) of Pseudomonas aeruginosa was investigated. The ability of OEP to consume the hemolytic complement (CH50) of guinea pig serum (GPS) was relatively lower than zymosan, a potent activator of the alternative pathway (AP). The profiles of the complement consumption with OEP in GPS suggested that classical pathway (CP) activation seems to occur in addition to the AP-activation, because the consumption of the C3-C9, i.e., C3 total was relatively higher than those of C1, C4 and C2. Further results stated below is confirmed this. OEP consumed complement in certain extent in C4-deficient GPS and also in factor B- or D-depleted GPS. In these sera either pathway of the AP or the CP was operated. However, OEP consumed complement quite in full extent in the EGTA-chelated serum, in which AP-activation took place in normal level. OEP was therefore found in this experiment to activate only the AP. The conversion of C3 in immunoelectrophoresis which is a evidence of the CP-and/or AP-activation was observed with OEP. It was suggested that the antibody against OEP did not participate in the consumption of complement, because the GPS priorly absorbed with OEP-coated sheep RBC, was retained fully to react with OEP. It was discussed that the active site of OEP as to the complement activation located on the LPS portion of OEP.

Animals↗