Biomedical subjects

K Itokazu

Publications and source records attributed to K Itokazu.

10 recordsLinked to original sources

PubMed1999

Incidence of Haemophilus influenzae in the throats of healthy infants with different feeding methods.

BACKGROUND: Haemophilus influenzae is the major cause of otitis media and lower respiratory tract infection in childhood. In the presence of human milk, which contains numerous host defense factors, Haemophilus influenzae may be inhibited in attaching to and colonizing pharyngeal cells. We investigated the incidence of H. influenzae in the throats of 162 healthy infants with different feeding methods: 70 breast-fed, 49 mixed-fed and 43 formula-fed infants. METHODS AND RESULTS: Haemophilus influenzae was identified using standard microbiological procedures and the API NH system. The incidence of H. influenzae in breast-fed infants, mixed-fed infants and formula-fed infants was 0, 0 and 7.0% respectively. CONCLUSION: The results suggest that the colonization of H. influenzae in the throat was inhibited by the presence of breast milk.

Bacterial Adhesion↗

PubMed1998

[Detection of enterovirus 70 in acute hemorrhagic conjunctivitis by PCR-stringent microplate hybridization method].

The surveillance of acute hemorrhagic conjunctivitis (AHC) caused by enterovirus 70 (EV70) is insufficient because of the difficulties of virus isolation using conventional methods. In this study, reverse transcription-polymerase chain reaction (RT-PCR) and stringent microplate hybridization (SMH) methods were assessed for the detection of EV70 in conjunctival swabs collected from the patients of AHC which broke out in Miyazaki Prefecture in 1990 and Okinawa Prefecture in 1994. Furthermore, with the use of the SMH method, we compared the genetic homogeneity of EV70 detected from the samples collected in 1990 and 1994 to the J670/71 strain isolated approximately 25 years ago. A similar investigation was performed between recent coxsackievirus A24 variant (CA24v) and EH24/70 strain, a standard strain of CA24v that was isolated approximately 25 years ago. As a result, RT-PCR products were detected in 26 of 34 conjunctival swabs collected in Miyazaki Prefecture in 1990. Likewise, RT-PCR products were detected in 9 of 10 conjunctival swabs collected in Okinawa Prefecture in 1994, and these RT-PCR products were all identified as EV70 by SMH method. Such a high level of virus recovery from specimen shows the evidence of usefulness of these techniques. Recent EV70 showed a reaction of about 60% to a probe synthesized with J670/71. Likewise, recent CA24v showed a reaction of about 30% to a probe synthesized with EH24/70. These finding are highly suggestive of the progress of viral mutation.

Conjunctivitis, Acute Hemorrhagic↗

PubMed1998

A survey on the vaccination practice for perinatal hepatitis B virus infection at a clinic in Okinawa, Japan.

The prevalence of hepatitis B (HB) virus carrier mothers and the HB immunization rate at a clinic in Okinawa prefecture were investigated. The prevalence of HB virus carrier mothers during 1981-1985, 1986-1994 and 1995-1997 were 3.5%, 2.3% and 1.1% respectively. According to the national prevention program, passive-active immunoprophylaxis with HB immune globulin (HBIG) and HB vaccine was carried out for 12 infants born to HB virus carrier mothers positive for HBe antigen (high risk infants) and for 24 infants born to carrier mothers negative for HBe antigen (low risk infants) since 1986. The immunization rate of high risk infants and low risk infants were 100.0% and 91.7%, respectively. A follow up system for the prevention program and assessment of vaccination schedule will be necessary to improve HB immunization rate of low risk infants in the future.

Ambulatory Care Facilities↗

PubMed1996

Iron status of newborns born to iron deficient anaemic mothers.

Haemoglobin, serum iron, total iron binding capacity, and serum ferritin were determined in newborns of 16 mothers with iron deficiency anaemia, 28 mothers with non-anaemic iron deficiency, and nine mothers with normal haemoglobin and serum ferritin levels. The results showed that there were no significant differences in the mean values of haemoglobin, serum iron, and total iron binding capacity among the newborns in the three groups. However, the mean value of serum ferritin differed significantly among the three groups, with the lowest values found in newborns of mothers with iron deficiency anaemia.

Adolescent↗

PubMed1985