Biomedical subjects

K Izdebska-Szymona

Publications and source records attributed to K Izdebska-Szymona.

11 recordsLinked to original sources

PubMed1992

Staphylococcal lipase affects phagocytosis of Staphylococcus aureus by human granulocytes and monocytes.

The rate of immunological and non-immunological phagocytosis of staphylococci by lipase pre-treated human granulocytes and monocytes was compared. It was found that the effect of this enzyme on two types of cells is opposite. Lipase decreases phagocytosis by granulocytes and increases by monocytes. The revealed differences between phagocytosing cells studied prompted us to investigate the influence of lipase on Fc receptors on these cells (rosette EA test). The different susceptibility of Fc receptors on non-activated phagocytes to lipase was found. This could be at least partially responsible for the difference observed between phagocytic activity of granulocytes (decreased) and monocytes (increased) pretreated with staphylococcal lipase. Inactivated enzyme showed a similar effect as active enzyme in the case of granulocytes. However, inactivated enzyme had no effect on rosette formation by lipase pretreated monocytes, indicating an enzymatic effect.

Dose-Response Relationship, Immunologic↗

PubMed1991

Zinc affects humoral and cellular response in mice.

The aim of the presented work was to elucidate whether supplementation with zinc affects the immunological response in BALB/c mice. Zinc was used as ZnCl2 in concentrations of 10(-4); 10(-5); 10(-6) M in PFC and migration-inhibition test. It was found that the numbers of anti-SRBC antibody-producing cells in mice injected with zinc were greater than in the control ones. This enhancement of PFC was proportional to the concentration of zinc. ZnCl2 itself inhibited target cell migration in concentration 10(-4) M but had no effect at 10(-5) and 10(-6) M. Zinc in all investigated concentrations promoted the action of suboptimal as well as optimal doses of PHA and enhanced target cell migration inhibition. It was determined that ZnCl2 in concentration 10(-4) M activated mouse lymphocytes for migration inhibitory factors production. We postulate that zinc may enhance the effectiveness of anti-infectious immunity.

Animals↗

PubMed1988

Factors influencing the adhesive properties of Pseudomonas aeruginosa.

The aim of this study was to evaluate different factors which may influence surface and adhesive properties of Pseudomonas aeruginosa strains isolated from patients with urinary tract infections. P. aeruginosa strains exhibited moderate surface hydrophobicity as shown by "salting out" with ammonium sulfate and hydrophobic interaction chromatography. The ability to haemagglutinate red blood cells by P. aeruginosa strains was increased when the cells were treated with papain and neuraminidase. The ability of all tested strains to attach to plastic and glass surfaces was independent on incubation temperature. There was no significant difference in the ability of any particular P. aeruginosa strains to adhere to Vero cells in culture. In this study no correlation between hydrophobic properties, intensity of haemagglutination, and ability to attach to Vero cells in culture was observed.

Bacterial Adhesion↗

PubMed1988

Comparison of some adhesive properties of Pseudomonas aeruginosa strains isolated from respiratory and urinary tract infections.

The aim of the paper was the comparison of adhesive properties concerning pathogenic potential of P. aeruginosa strains isolated from the patients with respiratory tract infections and from the patients with urinary tract infections. It was stated that P. aeruginosa strains had no haemagglutinating properties when cultured on a solid medium. Bacteria cultured in a liquid medium showed an increase of these properties in 48 h cultures as compared with 24 h cultures. They were not sensitive to heating. The haemagglutinating properties of the most strains were inhibited by D-mannose. These results seem to suggest that in P. aeruginosa strains fimbriae play an important role in adhesion. On the other hand, the mechanism of adhesion is not uniform as shows mannose-sensitivity of some strains and its lack in the other haemagglutinating strains. The most effective agglutination of human erythrocytes seems to be caused by the species specificity of the individual strains isolated from humans. The higher attachment of P. aeruginosa strains isolated from the urinary tract infections than those from respiratory tract infections to "Vero" cells suggests that these two strains populations may differ in their pathogenic potential to various tissues.

Animals↗

PubMed1975