Biomedical subjects

K J Hartman

Publications and source records attributed to K J Hartman.

4 recordsLinked to original sources

PubMed1999

Genome-scale cloning and expression of individual open reading frames using topoisomerase I-mediated ligation.

The in vitro cloning of DNA molecules traditionally uses PCR amplification or site-specific restriction endonucleases to generate linear DNA inserts with defined termini and requires DNA ligase to covalently join those inserts to vectors with the corresponding ends. We have used the properties of Vaccinia DNA topoisomerase I to develop a ligase-free technology for the covalent joining of DNA fragments to suitable plasmid vectors. This system is much more efficient than cloning methods that require ligase because the rapid DNA rejoining activity of Vaccinia topoisomerase I allows ligation in only 5 min at room temperature, whereas the enzyme's high substrate specificity ensures a low rate of vector-alone transformants. We have used this topoisomerase I-mediated cloning technology to develop a process for accelerated cloning and expression of individual ORFs. Its suitability for genome-scale molecular cloning and expression is demonstrated in this report.

Animals↗

PubMed1993

Postpartum pyometra: a case report.

Pyometra is an uncommon result of endometritis. The diagnosis of pyometra is made when a collection of pus is found within the endometrial cavity. Antibiotic administration and drainage of the pus are essential to resolve this condition. A report of a case of pyometra is presented and a technique for drainage is described. This case demonstrates the use of office-based diagnostic ultrasonography to narrow the differential diagnosis and guide the drainage procedure, as well as the use of simple ingenuity to improve patient care.

Adult↗

PubMed1992

Obstetric ultrasound by family physicians.

Obstetric ultrasound examination is a useful diagnostic procedure for family physicians who select appropriate equipment, observe indications, understand limitations, and work toward performance mastery. The knowledge obtained during an examination assists clinical decision making and reduces liability. This is particularly true for rural and underserved communities where family physicians provide the majority of prenatal and comprehensive perinatal care. Mastery of obstetric ultrasound techniques can also lead to developing amniocentesis skills and serve as an aid to external cephalic version. The equipment does not require extensive maintenance and is available at all hospitals. Many offices and group practices have found purchase of this equipment to be cost effective. Based on clinical experience in family practice and a review of the medical literature, an approach to skill acquisition and quality assurance is described.

Amniocentesis↗

PubMed1990

Relationship between liver tumors and age in brown bullhead populations from two Lake Erie tributaries.

We compared liver tumor frequencies, and age and length characteristics of brown bullheads (Ictalurus nebulosus) of greater than 250 mm total length from two Lake Erie tributaries. Bullheads taken from Old Woman Creek (n = 144) had no grossly observable liver tumors, while those collected in the highly industrialized Black River (n = 532) had a 30% frequency of grossly visible liver tumors during 1981-1982. Liver lesions diagnosed histologically in a randomly collected sample (n = 125) of brown bullheads from the Black River included both biliary and hepatic lesions, with cancerous neoplasms occurring in 38.4% of the fish. Black River bullheads of combined ages 4 and 5 had a significantly (p less than or equal to 0.05) greater prevalence of biliary carcinomas (35.5%) than those of ages 2 and 3 combined (18.4%). Biliary carcinoma was significantly more prevalent than hepatocellular carcinoma in age 4 fish (sexes combined) and in males of ages 3 and 4. The prevalence of hepatocellular carcinoma was significantly higher in females than in males. Age distributions of bullheads differed significantly between the two sites, while length distributions were similar. No brown bullheads of ages 6 or 7 were collected in the Black River, while these age groups composed 18% of the catch in Old Woman Creek. Brown bullheads of age 5 were almost six times more numerous in the Old Woman Creek than in Black River collections. These age and length distributions are consistent with the hypothesis that brown bullheads in the Black River were subjected to an age-selective mortality associated with high prevalences of liver carcinoma.

Aging↗