Specificity of antisera to oestrogen-azo-protein conjugates: the influence of tautomerism and carrier proteins.
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Biomedical subjects
Publications and source records attributed to K J Hruska.
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The application of gel filtration-centrifugation to the separation of free and protein-bound ligands in radioimmunoassay is described. The method is simple and rapid. A large number of samples can be handled simultaneously and the bount radioactivity is directly transferred to the scintillation vial. Using a 9 X 36 mm column of Sephadex G-50 (coarse), 400 mul of the reaction mixture can be separated during 2 min centrifugation.
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