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Biomedical subjects

K J Ranadive

Publications and source records attributed to K J Ranadive.

At least 37 records · Page 2Linked to original sources
PubMed1976

Experimental studies on betel nut and tobacco carcinogenicity.

Sun-dried Mangalore betel nut extracts in water and in DMSO, and sun-cured Vadakkan tobacco extract in DMSO, were tested for their carcinogenic potency. Inbred Swiss and C17 mice and golden hamsters were used for the experiments. Control animals treated with either DMSO or water did not show any changes at the sites of administration. On subcutaneous administration of betel nut extract, 60% of Swiss mice developed transplantable fibrosarcomas at the site of injection. Skin application of DMSO extracts of tobacco and of betel nut separately did not result in skin lesions in C17 mice; but when a mixed DMSO extract of tobacco and betel nut was used, skin papilloma and epidermoid carcinoma developed in some animals. Similarly, hamster cheek pouches painted with a DMSO extract of tobacco alone did not develop malignant atypia whereas those painted with a DMSO extract of betel nut showed early malignant changes. DMSO extract of a mixture of tobacco and betel nut positively increased the incidence of early malignant changes in the hamster cheek pouch, indicating the enhancing effect of betel nut in carcinogenesis.

Animals↗
PubMed1975

Circulating levels of prolactin in human breast cancer.

Serum prolactin concentrations were measured by radioimmunoassays in 98 patients with established carcinoma of breast, 12 patients with cystic mastitis and 10 patients with gynaecomastia and compared with that of age matched normal control women. The serum prolactin levels in the patients with breast cancer, gynaecomastia or cystic mastitis were observed to be similar to that in normal women. It was interesting to note that the levels of prolactin in the luteal phase of the cycle were higher than that in the early follicular phase in normal women.

Adult↗
PubMed1975

Activation of murine leukaemia virus under different physiological conditions.

The leukaemic lesions in intact and ovariectomized mice of strain ICRC, induced with 20-methylcholanthrene (20-MCA) in combination with or without hormones were investigated for the presence of mouse leukaemia virus (MuLV) by (i) bioassays and (ii) electron microscopy. The different experimental groups treated with 20-MCA were (i) intact females, (ii) ovariectomized females, (iii) ovariectomized females with pituitary graft, (iv) ovariectomized females with 10 mug oestradiol/day for 30 days and (v) ovariectomized females with 1 mug oestradiol together with 1 mg progesteron/day for 30 days. It was possible to transmit nearly all these experimentally induced leukaemias to syngeneic mice through acellular extracts, compared with very poor transmissibility of spontaneous leukaemias in the ICRC strain, indicating functional activation of viral agents on combined treatment with carcinogen and hormones. Potency of the acellular leukaemic extract from the mice of group (ii) without the ovarian hormones was much weaker than that from mice of the other experimental groups. The leukaemogenic activity of MuLV was enhanced on serial transmission in syngeneic hosts. Leukaemic lesions of ovariectomized mice treated with 20-MCA and oestradiol were also transmissible to the sucklings of allogeneic mice of strain C3H-MTV, C57-BL and Dba-MTV. The cell-free supernatant medium of the cultures of these leukaemic lesions induced leukaemias on back inoculation into syngeneic mice. Electron microscopic studies of lesions induced with carcinogen and oestradiol consistently showed abundant intracytoplasmic type A particles. Numerous intracytoplasmic type A particles as well as some type B particles were found in the leukaemic tissues of ovariectomized females treated with MCA and oestradiol combined with progesterone. Type C particles, characteristic of MuLV were seen in the leukaemic tissues of all other experimental groups. These findings indicate a significant influence of the physiological condition of the host, particularly the hormonal make up, on expression and activity of specific viral agents.

Animals↗
PubMed1974

Ectopic production of human chorionic gonadotrophin by human breast tumours.

The incidence of tumours ectopically producing the human chorionic gonadotrophins was studied in patients with breast cancer. Specific radioimmunoassay of subunits of HCG was utilized. Nine out of 65 patients with carcinoma of breast showed the presence of circulating HCG. Patients with other pathological conditions of breast tissue did not show any evidence of circulating HCG.

Adenofibroma↗
PubMed1973

Influence of hormones and chemical carcinogen on murine leukaemia.

Leukaemogenesis induced with chemical carcinogens and hormones was studied in intact and ovariectomized mice of the ICRC strain which is susceptible to spontaneous development of both breast cancer and leukaemia and the Strong A strain susceptible only to breast cancer and not to leukaemia. In ovariectomized females oestradiol was administered at two dose levels (i) 1 μg oestradiol/day for 30 days, (ii) 10 μg oestradiol/day for 30 days. The effect of oestradiol (1 μg/day) and progesterone (1 mg/day) for 30 days was also studied. In one group, two pituitaries of the syngeneic male mice were implanted subcutaneously on the right inguinal pair of mammary glands. Enhancing effect of 20-MCA on leukaemogenesis was seen in intact strain ICRC mice and not in ovariectomized mice. However, administration of hormones, either oestradiol alone or in combination with progesterone, or by the way of pituitary grafts, to these carcinogen treated ovariectomized females increased the incidence of leukaemia with a shorter latent period. Although 20-MCA could induce leukaemogenesis in Strong A ovariectomized females, further treatment with hormones, either with pituitary graft or with oestradiol, failed to promote leukaemogenesis. The highest leukaemia incidence in strain A ovariectomized females was observed in the group treated with a balanced dose of oestradiol and progesterone. The present experimental findings in the ICRC and Strong A strains suggest specific differential responses of different strains of mice to the action of carcinogen and hormones for the induction of leukaemogenesis.

Animals↗