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Biomedical subjects

K Jacobs

Publications and source records attributed to K Jacobs.

At least 37 records · Page 2Linked to original sources

Rapid hybridization kinetics of DNA attached to submicron latex particles.

We describe a novel method for attaching any DNA molecule to submicron latex beads and characterize the hybridization kinetic properties of these bead-DNA conjugates. The conjugates hybridize to DNA in solution with rates comparable to homogeneous hybridization reactions, are compatible with common hybridization conditions and are conveniently manipulated. They should thus serve as useful reagents for the fractionation and characterization of DNA and RNA.

Bacteriophage lambda

Marketing occupational therapy.

Marketing is emerging as an important aspect of the delivery of health care services, including occupational therapy. An understanding of marketing and a knowledge of how to apply its principles will permit therapists to keep pace with the changing health care environment. This article introduces terminology, strategies, and applications of marketing.

Advertising

Isolation and characterization of cDNA clones for the 35-kDa pulmonary surfactant-associated protein.

A group of 35,000-dalton sialoglycoproteins is the major non-serum protein component of pulmonary surfactant. Tryptic fragments of these proteins were sequenced, and oligonucleotide probes were synthesized based on the amino acid sequences. A human lung cDNA library was then screened using the oligonucleotide probes, and clones coding for these proteins were identified and characterized. By in vitro transcription-translation experiments we have associated individual clones with particular proteins. The data suggest that co-translational modifications of two primary translation products account for many of the isoforms observed by two-dimensional gel electrophoresis in the precursors of 35,000-dalton sialoglycoproteins.

Amino Acid Sequence

Adult ceroid-lipofuscinosis: diagnostic value of biopsies and of neurophysiological investigations.

In a sibship of ten, three brothers presented with an adult form of ceroid-lipofuscinosis. The diagnosis was confirmed by necropsy of the first patient and was made by electron microscopy of eccrine sweat glands and of skeletal muscles in the two others. Somatosensory evoked potentials were characterised by biphasic, nearly monophasic, very high voltage complexes totally unlike those found in normal controls. Similar sensory evoked potentials were however recorded in other types of ceroid-lipofuscinosis. While electron microscopy of easily available tissues gives fairly specific results, sensory evoked potentials can bring supportive diagnostic evidence in adult ceroid-lipofuscinosis.

Adult

Amino acid analysis of elastin hydrolysates using a lithium citrate gradient: quantification of elastin from whole lung.

A method to quantitate the elastin content in lungs is described. Desmosine and isodesmosine, the cross-linking amino acids unique to elastin, were measured directly from acid hydrolysates of hamster and human lungs by amino acid analysis. A Durrum D-500 (Dionex) amino acid analyzer, with a modified lithium citrate buffer gradient was used. Results compared favorably with those obtained by gravimetric and microenzymatic techniques, and the method described could be used for the quantification of elastin in biopsy specimens

Amino Acids

Deliberate hypotension and anesthetic requirements of halothane.

Following determination of minimum alveolar anesthetic concentration (MAC) of halothane during normotension, 18 dogs were divided into three groups. Dogs in each group then received either pentolinium, trimethaphan, or sodium nitroprusside, and mean arterial pressure was decreased to 60% of control values for 1 hour. The MAC of halothane was determined during hypotension and then arterial pressure was returned to control values and MAC was redetermined at normotension. The MAC of halothane decreased significantly during hypotension in all three groups: in the pentolinium group from a control MAC of 1.2 +/- 0.14 (SEM) to a MAC of 0.7 +/- 0.11 during hypotension, in the trimethaphan group from a control MAC of 1.02 +/- 0.06 to a MAC of 0.72 +/- 0.07 during hypotension, and in the nitroprusside group from a control MAC of 1.02 +/- 0.07 to a MAC of 0.73 +/- 0.07 during hypotension. Upon return of mean arterial pressure to normotensive levels, MAC of halothane returned to control values in dogs given pentolinium and trimethaphan, but in dogs given nitroprusside MAC remained at the hypotensive value. There was no correlation between the changes in MAC and carotid blood flow or cardiac index. These data indicate that anesthetic requirements decrease during deliberate hypotension irrespective of the drug used to produce hypotension.

Anesthesia, Inhalation

Adrenomyeloneuropathy and adrenoleukodystrophy in two brothers.

A 19-year-old patient presented with spastic paraparesis and peripheral neuropathy without clinical or biochemical signs of adrenal insufficiency. The diagnosis of adrenomyeloneuropathy was supported by typical ultrastructural findings in peripheral nerve and endomysial nerve bundles and by a positive family history. Indeed his younger brother died at age 12 from a demyelinating disease of the central nervous system. Cutaneous nerve bundles and postmortem examination limited to the brain demonstrated features specific for adrenoleukodystrophy. The various features of both disorders are discussed. Their occurrence in the same sibship pleads strongly for a very close relationship.

Adrenal Insufficiency

Protease and antiprotease responses in lung and peripheral blood in experimental canine pneumococcal pneumonia.

We studied the effects of pulmonary infection with type III pneumococcus in dogs on the degree of granulocyte elastase-like esterase activity and on the degree of antiproteases in peripheral blood and lung lavage fluid. In 9 infected dogs the peripheral blood granulocyte esterase activity increased from a preinfection level of 361.3 +/- 51 units/10(8) leukocytes to 593.8 +/- 80 units/10(8) leukocytes (mean +/- SE) 24 h after pneumonia had been induced (p less than .02). Parallel changes were measured in granulocytes obtained from bronchial lavage fluid. The antitrypsin activity and esterase inhibitory activity of peripheral blood increased by 15% after infection was induced. The esterase inhibitory activity of lung lavage fluid increased from a preinfection level of 776 +/- 160 units/mg of lavage protein to 1,390 +/- 200 units/mg of lavage protein (p less than .05). These findings indicate that whereas both circulating and lung lavage granulocytes contain increased concentrations of proteolytic activity during infection, serum antiproteases and, to a larger extent, lung antiproteases, also are increased.

Animals

[The blind as a factor in the labour divisioned world (author's transl)].

The problems faced by blind adolescents when leaving special schools to enter employment differ considerably from those encountered by people who lost their sight during adulthood. The realisation of the fact that their blindness leaves little occupational choice often results in job apathy. It is, however, especially work capacity and occupational activity which serve again and again, in discussions on the full integration of the blind, as proof of the visually impaired's equal position within society. In particular W. Thimm has proved that blindness has a decisive impact on the social position of the individual blind person as well as the whole disability group of the blind, because this is essentially determinated by the employment status. The individual job performance of some blind is generalised too rapidly. The fear that jobs carried out by the blind are jeopardised by the increasing automation is certainly not unfounded, especially in large enterprises. At present, however, it is not possible to make a prognosis on whether in the future job opportunities for the blind will be available or not in certain branches of industry or in specific branches of production (this varies from one firm to another and is dependent upon many other factors). Consequently, it seems to be necessary for institutions involved in the vocational training of the blind to adjust in due course to the new situation in the theoretical and practical implementation of rehabilitation.

Blindness