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K Jaroszewicz

Publications and source records attributed to K Jaroszewicz.

18 recordsLinked to original sources
PubMed1991

Human stored blood inositol phospholipids.

The total amount of phospholipids of the stored blood erythrocytes does not change during the first week of storage. After the second and the third week of storage the changes are only insignificant. During the fourth week this amount decreases by 25%. Detailed analysis of inositol phospholipids shows that balance of the phosphorylation-dephosphorylation cycle is shifted towards dephosphorylation. The decrease in phosphatidylinositol-4,5-bisphosphate (PtdIns-4,5-P2) is accompanied by the increase in phosphatidylinositol-4-phosphate (PtdIns-4-P) and phosphatidylinositol (PtdIns). The total amount of inositol phospholipids does not change. The increase in PtdIns-4-P is accompanied by the appearance of echinoidal forms of erythrocytes. The results of this study suggest that PtdIns-4-P can be considered as one of the important factors which determine the shape of erythrocytes.

Adult↗
PubMed1986

Turnover of phosphomonoester groups and compartmentation of polyphosphoinositides in human erythrocytes.

The turnover of phosphomonoester groups of phosphatidylinositol 4-phosphate (PtdIns4P) and phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] was investigated in human erythrocytes by short-term labelling with [32P]Pi. The procedure applied ensured a quantitative extraction of erythrocyte polyphosphoinositides as well as their reliable separation for the determinations of pool sizes and specific radioactivities. The pool sizes of phosphatidylinositol (PtdIns), PtdIns4P and PtdIns(4,5)P2 are 25, 11 and 44 nmol/ml of cells respectively. Under steady-state conditions, the phosphorylation fluxes from [gamma-32P]ATP into PtdIns4P and PtdIns(4,5)P2 are in the ranges 14-22 and 46-94 nmol X h-1 X ml of cells-1 respectively. Only 25-60% of total PtdIns4P and 6-10% of total PtdIns(4,5)P2 take part in the rapid tracer exchange, i.e. are compartmentalized. In isolated erythrocyte ghosts, the turnover of PtdIns4P approximately corresponds to that in intact erythrocytes, although any compartmentation can be excluded in this preparation. Under the conditions of incubation employed, the turnover of PtdIns(4,5)P2 is more than one order of magnitude smaller in isolated ghosts than that obtained for intact erythrocytes.

Adenosine Triphosphate↗
PubMed1983

Composition of phospholipids in various rat tissues.

Total phospholipids and their various classes in erythrocytes, the blood plasma, liver, lung, myocardium, spleen and skeletal muscles were determined quantitatively by means of two-dimensional thin-layer chromatography.

Animals↗
PubMedDate not supplied

Phospholipid composition of plasma and erythrocytes in the neonate.

The total phospholipids and their various classes in erythrocytes and blood plasma were determined quantitatively by means of two-dimensional thin-layer chromatography. The total amount of phospholipids in neonate plasma was approximately half of that found in adult plasma, however, the amount of phospholipids in erythrocytes of the neonate was significantly higher. The differences were observed in some classes of phospholipids in the plasma and erythrocytes of neonates as well as adult human beings.

Adult↗