PubMed Health⌕ Search

Biomedical subjects

K Joshi

Publications and source records attributed to K Joshi.

At least 109 records · Page 6Linked to original sources
PubMed1990

Histomorphological criteria for prognosis in breast cancer.

One hundred cases of carcinoma breast were analysed with regard to histological features and clinical follow-up. Bad prognostic factors included tumour size larger than 5cms; absent or minimal intraduct component in the tumour and absent or minimal periductal elastosis. Conversely significant intraduct component in the tumour and periductal elastosis were associated with better survival. Degree of lymph nodal involvement did not influence the outcome. One reason for this unexpected finding could be that patients with more than 3 lymph node involvement were given additional chemotherapy.

Breast Neoplasms↗
PubMed1990

Interactions of macrophages from in vivo stimulated guineapigs & the trophozoites of Entamoeba histolytica.

The interactions between peritoneal macrophages obtained from guineapigs immunized with a Sephadex chromatographed fraction (FI) of crude amoebic extract proteins and trophozoites of axenic E. histolytica (NIH:200) were studied in vitro in the presence or absence of anti FI antiamoebic antibodies. Unstimulated macrophages killed trophozoites to some extent. Incorporation of anti FI antiamoebic antibodies or IgG of FI antibodies in the amoebae macrophages mixture significantly enhanced the cytotoxic potentials of both unstimulated and in vivo stimulated peritoneal macrophages. Transmission electron microscopy revealed a close contact between macrophages and E. histolytica trophozoites. A large number of lysosomal vacuoles were seen accumulating near the contact areas. The contact between peritoneal macrophages and amoebic trophozoites in the presence of anti FI antibodies led to extensive degeneration of amoebic trophozoites. The antiamoebic antibodies induced cytotoxicity by macrophages in an opsonic manner. The data suggest that antiamoebic IgG binding to the trophozoites by its Fab portion and to the macrophages by its Fc portion initiated the cytotoxic mechanism(s) of macrophages.

Animals↗
PubMed1989

Role of culture for mycobacteria in fine-needle aspiration diagnosis of tuberculous lymphadenitis.

A total of 390 cases of tuberculous lymphadenitis was subjected to fine-needle aspiration cytology; 100 of the aspirates were subjected to culture for mycobacteria. The overall acid-fast bacilli (AFB) positivity in smears was 23.58%, with a maximum positivity of 32.94% in smears with both necrosis and granuloma. The overall rate of isolation of mycobacteria on culture was 35%. Mycobacteria were more frequently isolated from caseating lesions (40%) than noncaseating lesions (9%). Caseating lesions with granuloma had the highest AFB (smear and/or culture) positivity at 52%. Mycobacterium avium infection was diagnosed by culture in one case.

Biopsy, Needle↗
PubMed1989

Nuclear morphometric analysis of non-Hodgkin's lymphoma.

Nuclear morphometric data on resin-embedded sections have been utilized to classify 50 cases of non Hodgkin's lymphoma (NHL) and to compare with morphological classification. Nuclear morphometric parameters studied were maximum diameter (Dmax), area, perimeter, form factor (FF) and nuclear contour index (NCI). The first three are determinants of the size, and the latter two indicate the shape of nuclei. Based on Dmax, small (4.5-5.5 microns), intermediate (5.5-7.0 microns) and large (7.0-12.0 microns) cell lymphomas were identified, which correlate with the low, intermediate and high grade lymphomas respectively of the Working Formulation. Determination of form factor further helped to subclassify these cases into lymphomas of B cell other than of follicular centre cell origin (FF = 0.7-1.0), follicular centre cell lymphomas (FF = 0.55-0.75) and T-cell lymphomas (FF less than 0.55). Pleomorphism factor is an objective assessment of the degree of morphological pleomorphism and is generally proportional to the grade of malignancy.

Cell Nucleus↗
PubMed1989

Congenital cyst of Krause's gland.

Cysts of Krause's glands usually occur as retention cysts in cicatricial conditions of the conjunctiva. We report a rare case of congenital cyst of Krause's gland in a 2-year-old boy.

Child, Preschool↗
PubMed1986

Cellular proliferation in the rat mammary gland during pregnancy and lactation.

Cellular proliferation during different stages of pregnancy and lactation in the rat mammary gland was studied by use of autoradiography at the ultrastructural level or in combination with immunocytochemistry. The study consisted of two groups of animals, young 45-day-old rats undergoing their first pregnancy and old multiparous rats undergoing their third pregnancy. Both groups of animals were injected with [3H]thymidine, 2 hours before sacrifice. The timing of proliferative activity through pregnancy and lactation was similar in the two groups. A peak level of proliferation was observed on the 5th day of gestation and the rate of proliferation was at its lowest at the end of gestation. A second small peak was observed on the 3rd day of lactation. In virgin animals, both epithelial and myoepithelial cells showed a high level of proliferative activity. The epithelial cells continued to divide during all stages of their differentiation, throughout pregnancy and lactation. Myoepithelial cells showed active division in early pregnancy but their rate of proliferation declined as the cells acquired abundant cytoplasmic filaments for their contractile function. Intra-alveolar dendritic cells, which have been recently identified in the lactating rat breast, showed a high proliferative rate at the end of gestation.

Animals↗
PubMed1986

Cell proliferation in the human mammary epithelium. Differential contribution by epithelial and myoepithelial cells.

The ductal system of the human breast consists of epithelial, myoepithelial, and basal clear cells. By labeling ducts and alveoli dissected from reduction mammoplasty specimens with 3H-thymidine in vitro and labeling human breast organoids xenografted in nude mice in vivo, it was found that cellular proliferation in the human breast is virtually confined to epithelial and basal clear cells. A pulse label of 3H-thymidine in organ culture explants was followed over a period of time, and it was found that myoepithelial cells originate from a precursor cell population within the mammary epithelium after a number of cell divisions. Myoepithelial cells were not seen to divide when fully mature.

Adolescent↗
PubMed1985

Ultrastructural identification of Ia positive dendritic cells in the lactating rat mammary gland.

Dendritic cells which express Ia antigen have been demonstrated for the first time in the lactating rat mammary gland. Ultrastructurally, the dendritic cells appear as electron-lucent pale cells interspersed among the epithelial cells of the alveoli, forming a cell population distinct from classical macrophages. They show morphological resemblance to the dendritic cells of lymphoid organs as well as the Langerhans cells of skin. The Ia antigen has been localised by electron microscopic immunocytochemistry on the cell membrane and endocytotic vesicles and tubules. Ia positive cells are also seen in the stroma of the mammary gland. It is proposed that the dendritic cells of the mammary gland belong to the lineage of epidermal Langerhans cells and lymphoid dendritic cells, subserving an immunological role in the lactating breast.

Animals↗
PubMed1983

Ménétrièr's disease and trichobezoar of stomach--an unusual association.

Hypertrophic gastropathy (Ménétrièr's disease) is a clinical and pathological curiosity. It is often seen in association with a variety of other disease processes. The authors here present a previously undescribed association of Ménétrièr's disease with trichobezoar in the stomach and small intestine.

Adult↗
PubMed1980

Multiple sclerosing haemangiomas of the lung.

A case of mulitple sclerosing haemangiomas of the lung is described from a 40-year-old woman, who presented with haemoptysis. A chest X-ray revealed multiple circumscribed coin lesions in both lungs. A right upper lobectomy was done for diagnosis. The patient has remained well for 2 years after surgery. Multiplicity of tumour masses of pulmonary sclerosing haemangiomas is extremely rare and, although benign, may pose a great diagnostic problem.

Adult↗