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Biomedical subjects

K K Tripathi

Publications and source records attributed to K K Tripathi.

27 records · Page 2Linked to original sources

Bacteriology of cervix in cases of infertility: effect on human sperm.

Microorganisms such as Escherichia coli, Pseudomonas aeruginosa, and Bacillus subtilis isolated from cervices of infertile females possessed spermicidal activity. They also agglutinated the human spermatozoa in vitro and showed tail-to-tail agglutination. Cell-free supernatant of these organisms was found to be spermicidal but did not agglutinate spermatozoa in vitro. Spermicidal activity was increased with increase in age of the culture.

Bacterial Physiological Phenomena↗

Comparison of the bio-physical and biological properties of staphylococcal protein-A extracted by salt-washing and conventional procedure.

Staphylococcal protein A could be extracted in large amounts by simple stirring of the cells with physiological saline (pH 7.8). The amount of protein A obtained by the technique was found to be the same as obtained by lysozyme/lysostaphin techniques (45 micrograms/ml). The protein A extracted by salt-washing technique was of higher molecular weight (71,000) and differing in some biological properties like complement fixation from the protein A prepared by lysostaphin technique.

Adhesiveness↗

On the growth of Brucella species in presence of erythritol in defined and undefined media and amniotic fluid of human, cow and sheep.

Different concentrations of erythritol (25-1000 microng/ml) in defined medium were shown to enhance the growth of 75.00 to 91.66 per cent of Brucella abortus and 69.23 to 100.00 per cent of Brucella melitensis strains studied. Compared to these figures only 13.04 to 60.86% (in tryptose broth), and 41.40 to 62.50% (in glycerol dextrose broth), of Brucella abortus strains, 25.00 to 83.30 per cent (in tryptose broth) and 83.30 to 100.00 per cent (in glycerol dextrose broth) of Brucella melitensis strains showed increased growth over the control. 82.20 per cent of amniotic fluid samples from humans showed anti-brucella activity with lytic zones varying from 7-19 mm. Amniotic fluid from cows and sheep had no antibrucella activity.

Amniotic Fluid↗