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K Köhler

Publications and source records attributed to K Köhler.

At least 55 records · Page 3Linked to original sources
PubMed1994

Koch et al. reply.

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Journal Article↗
PubMed1994

Polyethylene glycol-potassium phosphate aqueous two-phase systems. Insertion of short peptide units into a protein and its effects on partitioning.

Two different tetrapeptides, AlaTrpTrpPro and AlaIleIlePro, were inserted near the C-terminus of the protein ZZT0. The Trp-rich peptide unit strongly increased both the partitioning of ZZT0 into the polyethylene glycol (PEG)-rich phase in a PEG-potassium phosphate aqueous two-phase system and its retention on PEG and propyl hydrophobic interaction chromatographic columns with potassium phosphate as eluent. Both the partitioning and the retention increased with increasing number of Trp-rich peptide units inserted into ZZT0. Insertion of Ile-rich tetrapeptide units affected the partitioning and retention to a much lesser extent. Partition data also indicated a folding of inserted Trp tetrapeptides units, probably to minimize their water contact.

Amino Acid Sequence↗