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K Kölble

Publications and source records attributed to K Kölble.

22 records · Page 2Linked to original sources
PubMed1987

Patient-specific heterogeneity of antinuclear antibodies as revealed by an isoelectric focusing immunoblot system.

Ninety-nine sera from patients with different rheumatic diseases (systemic lupus erythematosus, rheumatoid arthritis, progressive systemic sclerosis, and mixed and unidifferentiated connective tissue disease) were applied to a newly developed isoelectric focusing (IEF) immunoblot system for the demonstration of antinuclear antibodies. Nucleoproteins were separated according to their isoelectric points (pI) and immobilized onto nitrocellulose, and binding of serum antibodies was determined by an alkaline phosphatase labelled second antibody. 89.8% of all sera positive in indirect immunofluorescence assays with Hep 2 as substrate showed positive reactivity in IEF immunoblot. Furthermore, 88% of patients' sera negative on Hep 2 cells gave a positive reaction in IEF immunoblot. The predominant antibody banding pattern observed showed parallel bands in the acidic as well as the neutral pH ranges. Antibody specificities found in the IEF immunoblot system turned out to be patient-specific, but no marker antibody for a discrete disease entity was obtained. Even when monoclonal antibodies or WHO standard sera were applied to nuclear antigen they exhibited heterogeneity in their binding pattern. Bands with the same pI were observed using sera from patients with different rheumatic disease entities. Immunodeletion experiments suggest the recognition of identical antigenic proteins by the different patients' sera.

Antibodies, Antinuclear↗
PubMed1987

Improved in vitro diagnosis of allergy to Alternaria tenius and Cladosporium herbarum.

To improve the in vitro diagnosis of mould allergy 22 children suffering from allergic asthma caused by Alternaria tenius and/or Cladosporium herbarum as proven by bronchial provocation test were investigated. Partially purified, standardized mould preparations were used in radioallergosorbent test (RAST) with conventional and new update mould discs, mould-induced histamine release and immunoblotting. Updated RAST discs were found to be superior to the old-type discs for the detection of Alternaria but not Cladosporium sensitivity. In all patients except one, specific IgE-antibodies to the respective mould were demonstrated by immunoblotting. Mould-induced histamine release failed to prove sensitization in only two patients. No differences were found comparing histamine release from whole blood with release from isolated cells. The results demonstrate a high sensitivity of in vitro tests when purified and standardized extracts are used.

Allergens↗
PubMed1984

[Benign symmetrical lipomatosis in women. Association with alcoholic hepatopathy].

Benign symmetric lipomatosis or Launois-Bensaude syndrome is a condition characterized by diffuse, unencapsulated, painless growth of lipomatous tissue with a peculiar symmetric topology occurring predominantly in middle-aged men. In the present study a short survey of the clinical manifestations, differential diagnosis, current pathophysiologic theories, and therapy of this disease is given. From the literature published since 1950 112 observations are reviewed with respect to alcohol abuse in the premorbid period and other coexisting illnesses. Of the patients 70% were heavy drinkers and in over 45% significant liver damage was present. In addition, two rare cases of benign symmetric lipomatosis in women, aged 42 and 49 years, respectively, are presented. Both were alcoholics with hepatic sequelae. The possible existence of a female morphological subtype of benign symmetric lipomatosis is ventilated and its identity with Touraine-Renault's segmental lipomatosis of the trunk suspected.

Adult↗