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Biomedical subjects

K Kadoi

Publications and source records attributed to K Kadoi.

30 records · Page 2Linked to original sources
PubMed1994

The propagation of a porcine hemagglutinating encephalomyelitis virus in swine kidney cell cultures.

An established cell line, KSEK6, derived from swine embryo kidney proved to be a suitable host for the propagation of a hemagglutinating encephalomyelitis virus, strain 67N. Infected cells showed clear cytopathic effect as early as 24 hours incubation at 37 degrees C. Plaques easily visible to the naked eye were also produced under agar overlay medium. The infective titer of 3rd passage level in the cells was in the order of 10(8) PFU per ml. Detecting antibodies against this virus strain in swine sera was considered to be more accurate by neutralization test than by hemagglutination inhibition.

Animals↗
PubMed1992

Susceptibility of a line of dolphin kidney cell culture to several herpesviruses.

A cell line was established from cell cultures of kidney cortex of a pantropical spotted dolphin, Stenella attenuate. The replication of 6 strains of herpesviruses was studied in the cells. Five strains of them, herpes simplex virus type I and type II, equine rhinopneumonitis virus, infectious bovine rhinotracheitis virus and Aujeszky's disease virus, were grown fairly well in showing clear cytopathic effects and plaques under agar overlay medium.

Animals↗
PubMed1992

Viral susceptibility of an established cell line of swine embryo kidney.

The viral susceptibility of a cell line, named KSEK6, newly established from the kidney cortex of swine embryo was tested for the indication of CPE occurrences and also plaque formations. The multiplication of porcine adenoviruses was considerably high in the cells among the virus strains tested though plaques of these viruses were hardly visible under agar overlay medium. Two strains of swine enteroviruses, Aujeszky's disease virus and hemagglutinating encephalomyelitis virus also multiplied well in a similar order to those received in the other cells employed.

Adenoviridae↗
PubMed1992

A mechanism involved in the plaque enhancement effect of sodium thiosulfate for foot-and-mouth disease viruses.

A mechanism involved in the plaque enhancement effect of foot-and-mouth disease viruses (FMDV) by the addition of sodium thiosulfate (Hypo) in the agar overlay medium (AOM) previously reported was studied. It was experimentally proved that the diffusion of virus particles through agar overlay medium was enhanced when this salt was incorporated. Accordingly, the enlarged plaque formation was assumed to be caused by the enhanced diffusion of viral progenies produced in infectious centers during plaque assays.

Agar↗
PubMed1987

Hemagglutination with transmissible gastroenteritis virus.

Transmissible gastroenteritis virus grown in primary swine kidney cell cultures agglutinated erythrocytes from chicken, guinea pig and cattle but not erythrocytes from mouse and goose. The optimal incubation temperature was at 4 degrees C. The hemagglutination (HA) reaction was inhibited by specific antiserum. Some factors involved in the HA and HA-inhibition (HI) were investigated and standard HA and HI tests were established. HI antibody titers of individual pig sera showed a significant positive correlation with their neutralizing antibody titers.

Animals↗
PubMed1986

Plaque enhancement effect of sodium thiosulfate for foot-and-mouth disease viruses.

A plaque enhancement effect by the addition of sodium thiosulfate for foot-and-mouth disease viruses was demonstrated when this salt was incorporated in agar and in agarose overlay media. Most of the mechanism is obscure, however, as one of the effects is that sodium thiosulfate seems to interact in a reversible manner against the plaque inhibitor action of neutral red in cellular cytoplasm. A plaque inhibitor contained in agar could be removed in some degree by the addition of this salt.

Agar↗