PubMed Health⌕ Search

Biomedical subjects

K Kajikawa

Publications and source records attributed to K Kajikawa.

At least 37 records · Page 2Linked to original sources
PubMed1983

External skeletal fixation using methylmethacrylate. Current technique, clinical results, and indications.

External skeletal fixation using self-curing acrylic resin was applied by inserting A-O cortical screws into the diaphyseal region and A-O cancellous screws into the epiphyseal and/or metaphyseal regions. The position of the screws was designed to secure three-dimensional fixation. Points of insertion are selected that avoid infected bone, open wounds, thick muscles, and skin that is relatively mobile during joint motion. After reduction the acrylic resin rather than conventional metal linking devices is used for fixation of the screws. Once the fixation is completed, it is adjustable and so rigid that exercises for joint function are possible. The apparatus is light in weight and sufficiently compact to fit under clothing. In the 204 patients treated by this method, open tibial fractures occurred in 123; nonunion or delayed union in 28; metaphyseal and epiphyseal fractures in 30; femoral fractures in ten children; and miscellaneous fractures and other conditions in 13. Nearly all of the operations were successful. Three patients had refractures after removal of the fixation device.

Adult↗
PubMed1982

Gastric plasmacytoma: an immunologic and immunohistochemical study.

A case of IgG (lambda) secreting plasmacytoma arising in the stomach has been presented. Immunohistochemically, the tumor cells contained IgG-lambda immunoglobulin. Immunoelectrophoretic study on patient's serum revealed in the gamma zone the presence of M protein which reacted specifically with anti-IgG and anti-lambda antisera. Agar electrophoresis and immunofixation electrophoresis with anti-IgG and anti-lambda antisera showed multiple subbands. Neuraminidase treatment of the purified IgG from this patient resulted in gradual reduction of four subbands to two. Electrophoretic charge difference of the monoclonal immunoglobulin may be attributed to incompletely synthesized sugar chains attached to the protein core.

Blood Proteins↗
PubMed1982

Hyaline globules in yolk sac tumor. Histochemical, immunohistochemical and electron microscopic studies.

Four cases of yolk sac tumor of the testis and ovary were examined by histochemistry, immunohistochemistry, and electron microscopy, and particular attention was directed toward the close relationship between hyaline globules and alpha fetoprotein (AFP). The hyaline globules were easily identified as eosinophilic globular structures located in the cytoplasm of the tumor cell as well as in the extracellular space. They were considered to be of glycoprotein in nature by their histochemical staining property. By an indirect immunoperoxidase method, the majority of intracytoplasmic hyaline globules were positive for AFP. Electronmicroscopically, the hyaline globules appeared as electron dense bodies with no limiting membranes. They were divided into two types by their electron density and inner structure: one is an electron high dense body consisting of homogeneous or fine granular materials and the other is a moderately electron dense body composed of fine filamentous structures similar to the basement membrane materials. It is suggested that the former corresponds with the AFP positive hyaline globules produces by the tumor cells of visceral type, while the latter represents the AFP negative globules formed by the cells of parietal type in non-neoplastic counterparts.

Adult↗
PubMed1981

Ultrastructure of the mouse synovial membrane. Development and organization of the extracellular matrix.

The synovial membrane of the mouse knee joint was examined by electron microscopy and electron microscopic histochemistry, with special reference to the development of the extracellular matrix. In the embryonic synovium, the intercellular spaces were filled with hyaluronate and chondroitin sulfate. The formation of the early joint cavity appeared to be initiated by accumulation of hyaluronate and chondroitin sulfate in the synovial primordium. At the postnatal stage, the synovial primordium differentiated into a true synovial intima that could be easily identified by the presence of two distinct lining cells: fibroblast-like cells (B cells) and phagocytic cells (A cells). Simultaneously, the synovial intima provided the specialized extracellular matrix that was characterized by organized structures of microfibrils, collagen fibers, and fibrous long spacing fibers embedded in a large number of glycoproteins.

Animals↗
PubMed1981

Secretory granules of B-cells in the synovial membrane. An ultrastructural and cytochemical study.

Ultrastructural and cytochemical studies have been made on secretory granules of B-cells (fibroblast-like cells) in the knee-joint synovium. The secretory granules were membrane-bounded spherical or slightly elongated bodies, 150 to 350 nm (average 230 nm) in diameter and had a homogenous matrix with several cores. These granules were found in B-cells of all animal species examined; they were numerous in mice and rats, and few in guinea pigs, rabbits and man. Ultrastructural and cytochemical examinations revealed that the Golgi apparatus was involved in the formation of the secretory granules. Unlike lysosomes, they showed no acid phosphatase activity. The granule matrix was positively stained by Thiéry's periodic acid-thiocarbohydrazide-silver proteinate technique, and the cores were digested by protease. These findings suggest that the granule matrix contains mucopolysaccharide(s) and/or glycoprotein(s) and the core material is largely proteinaceous in nature.

Animals↗
PubMed1981

Myofibroblasts in fibrous tumors and fibrosis in various organs.

The morphology and population of myofibroblasts detected by electron microscopy in fibrous tumors and fibrosis in various organs have been examined. The myofibroblast was identified by characteristic features of the indented nucleus, bundles of abundant microfilaments with dense bodies, focal basal lamina and well-developed rough endoplasmic reticulum. It has been observed that myofibroblasts in a variety of fibrous tumors and fibrosis appeared predominantly in fibromatosis and reparative process of deep fibrous tissues and organs particularly in the lesions involving the fascia and subcutaneous tissue. It was suggested that myofibroblasts would be modified fibroblasts to which fibroblasts in the fascia and deeply situated fibrous tissue transformed during proliferation and differentiation.

Adult↗
PubMed1981

Distribution of fibrous long-spacing fibers in normal and pathological lymph nodes.

The occurrence and distribution of fibrous long-spacing fibers (FLS) of lymph nodes in normal and pathological conditions have been examined by electron microscopy. The FLS can be easily identified by the characteristic broad cross bands with a 90 to 120 nm period. In normal lymph nodes, the FLS were most often found in the stalk of the valve projecting into the lymphatic sinus and in the perivascular matrix, and less frequently in the wall of the vein, in the sinus wall and in the stroma of the medulla at the hilus. In pathological conditions, a large number of FLS appeared in the hyalinous matrix of lymphocyte depletion type of Hodgkin's disease. These fibers were also observed in reticular matrices of lymphosarcoma and mixed cellularity type of Hodgkin's disease as well as in granulation tissues. It was noted that FLS appeared to be formed in the regions where abundant reticular fibers were laid down. Compared with the ultrastructural and histological findings in various lesions of lymph nodes, the possible mechanism and significance of FLS were discussed.

Adult↗
PubMed1980

The effect of collagenase on the formation of fibrous long spacing collagen aggregates.

In order to elucidate the close relationship between the formation of fibrous long spacing fibers (FLS) and collagenase activity, electron microscopic studies on the rat skin using tissue culture technique were performed. In culture of newborn rat skin, a large number of FLS was formed in the dermis in which activity of endogenous collagenase was markedly elevated. Ethylenediaminetetraacetic acid (EDTA) supplemented to the culture medium completely arrested the formation of the FLS; subsequent exposure to bacterial collagenase resulted in the appearance of FLS in the same fashion. In another set of experiments utilizing exogenous enzyme digestion with bacterial collagenase or chondroitinase ABC on skins of 1-day-old and 1-year-old rats, it was demonstrated that the FLS formed by incubation with bacterial collagenase were found only in the regions of the dermis where reticular fibers were identified by light microscopy. There was no effect of chondroitinase ABC on FLS formation. These studies indicate that the FLS are formed in the presence of elevated endogenous collagenase and might be derived from reticular fibers which are degraded by collagenase.

Aging↗
PubMed1975

A case of watery diarrhea, hypokalemia and hypercalcemia associated with nonulcerogenic islet cell tumor of the pancreas.

A case of watery diarrhea, hypokalemia and hypercalcemia associated with an islet cell tumor was described. A 62-year old man exhibited frequent watery diarrhea and hypokalemia for two years. He had no peptic ulcer and serum gastrin level was normal. His serum calcium was abnormally high and serum phosphate was lowered. He had secretin-like activity in his plasma. Autopsy revealed a small islet cell tumor in the pancreas and several metastatic masses in the liver. Microscopic examination revealed the tumor cell was not beta, alpha nor D cells. By electron microscopy the secretion granules of the tumor cell resembled those of S, M and T cells. It was not possible to decide which of the tree cell types was responsible for the pancreatic cholera.

Adenoma, Islet Cell↗