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Biomedical subjects

K Kanclerski

Publications and source records attributed to K Kanclerski.

11 recordsLinked to original sources
PubMed1997

[Preliminary evaluation of the incidence and control of insects--pest control in Polish hospitals].

The evaluation of the infestation and methods of insect disease vectors control in 748 hospitals in Poland in the period of 1990 to 1995 were done. The insect species, places of their occurrence and control agents were analysed. Blattella germanica L. occurred most frequently (71% hospitals). Blatta orientalis and Monomorium pharaonis were found in 40% and 17% hospitals respectively. Kitchens, laundries and baths were most infested. Sometimes insects were found also in central sterilization units and operating theaters. Controls of insects in hospitals were performed one to four times a year mostly by spraying with residual formulation. The control agents contained pyrethroids (mostly permethrin, but also deltamethrin and cypermethrin) and carbamates (bendiocarb, propoxur). Baits with hydramethylnon, boric acid, methoprene and chlorpyrifos were used not very often. The authors suggest reduction in using the spraying agents. The baits are recommended because they delay the development of the resistance to pesticides in controlling insect populations and are safer.

Animals↗
PubMed1996

Serum antibody response to Staphylococcus aureus enterotoxins and TSST-1 in patients with septicaemia.

The prevalence of enterotoxins and toxic shock syndrome toxin (TSST-1) production in strains isolated from patients with Staphylococcus aureus septicaemia, and the serum antibody response in relation to toxin production in vitro of each isolate, were investigated. Among 63 strains of S. aureus isolated from the blood of patients with septicaemia, 51 from patients with superficial wounds and 49 from nasal carriers, 50-60% produced at least one of the enterotoxins A-D or TSST-1. The most frequent toxins produced were enterotoxins A and C and TSST-1. Among the 63 patients with staphylococcal septicaemia, 51 (81%) had a significant rise or a high antibody titre, or both, to at least one of the toxins. A positive serological response to toxin A was found in 78%, to enterotoxin B in 83%, to enterotoxin C in 80%, to enterotoxin D in 86% and to TSST-1 in 92% of the patients from whom the isolated strain produced the respective toxin. Antibodies against enterotoxins A, B, C and D and TSST-1 were also seen in 35%, 16%, 32%, 59% and 10%, respectively, in patients infected by strains that did not produce the specific toxin. Immunological cross-reactions between the toxins were demonstrated both in hyperimmune sera obtained from rabbits and in patients' sera, particularly between enterotoxins B and C. It is concluded that these potent toxins with superantigenic properties are produced in vivo during S. aureus septicaemia. No differences with regard to enterotoxin or TSST-1 production or antibody response were noted between patients with complicated versus uncomplicated septicaemia.

Adolescent↗
PubMed1990

[The role of surface structures of Streptococcus agalactiae in adhesion to epithelial cells].

Induced mutants of S. agalactiae which differed in surface structures were used for the study. The aim of using them was to try to correlate the presence of defined structures or surface properties with the ability of group B streptococci to attach to epithelial cells. The presence of protein antigen R conditioned strong binding of S. agalactiae cells to hydrophobic gel. Strains bearing clumping factor (CF) showed high surface hydrophobicity and presented compact growth in serum soft agar. However, there was no correlation between high surface hydrophobicity and the ability to adhere. Fibrinogen binding decreased the attachment to epithelial cells of CF-positive strains. Preincubation of bacterial cells with lectin (ConA) did not influence the attachment of S. agalactiae strains with protein surface antigen but increased the adhesion of the strains with polysaccharide antigen or untypable.

Animals↗
PubMed1989

Etiology of community-acquired pneumonia in children based on antibody responses to bacterial and viral antigens.

The serologic responses to bacterial and viral antigens were determined in paired serum samples from 336 children, ages 1 month to 15 years, with roentgenographically verified community-acquired pneumonia. Significant increases in antibodies against one agent were found in 40% and against two or more agents in 8% of the children. There were significant increases in antibodies against respiratory syncytial virus in 20%, viruses of the influenza-parainfluenza group in 6% and adenovirus in 3%. A serologic response to one or more of the pneumococcal antigens used (type-specific capsular polysaccharide, C-polysaccharide and pneumolysin) was demonstrated in 13% of the patients. Ten percent of the children had significant increases in antibodies against Mycoplasma pneumoniae. Only three patients had increases against Haemophilus influenzae type b and one each against Legionella pneumophila and Chlamydia. Respiratory syncytial virus was the predominant etiologic agent in young children whereas M. pneumoniae was more frequent in the older age group.

Adenoviruses, Human↗
PubMed1988

Serum antibodies to pneumolysin in patients with pneumonia.

Serum antibodies to purified pneumolysin were determined by enzyme-linked immunosorbent assay (ELISA) in paired samples from 406 adult patients with community-acquired pneumonia and in samples from 184 healthy controls. A high sensitivity (83%) was obtained in patients with blood culture-confirmed pneumococcal pneumonia. In patients with a tentative pneumococcal diagnosis based on culture of samples from the sputum or the nasopharynx, 45% were positive by ELISA. The difference likely reflected the different relevance of cultural findings for the diagnosis of pneumococcal pneumonia. A significant rise in ELISA titer was found in 17% of the patients. When the diagnosis was also based on high titers, 25% were positive. Pneumococcal pneumonia diagnosed by the pneumolysin ELISA was significantly more common in the patients with a more severe disease and who required hospitalization (21 versus 5% for outpatients). Younger patients were more often positive for pneumococci as determined by high titers, while older patients showed titer rises. Mixed infections with other infectious agents were not uncommon. The finding of low titers in acute-phase samples from positive patients and in the youngest and oldest age groups of healthy controls were unexpected, indicating that further studies on the role of pneumolysin in pneumococcal disease are warranted.

Adolescent↗
PubMed1987

A simple and exact two-point interpolation method for determination of haemolytic activity in microtiter plates.

A new haemolytic test based on direct reading of the optical density of unlysed erythrocytes was developed and adapted for use with microtiter plates and an appropriate reader. The relationship between the concentration of erythrocytes and the optical density was not strictly linear, but a high reproducibility of the curve was noted. There was a linear relation between the optical density of unlysed erythrocytes and -log2 of the haemolysin dilution in a wide range. The correlation coefficient was higher than 0.99. Because of this strong linearity, only two points of the two-fold dilution series, representing the dilutions before and after 50% lysis, were used to interpolate the exact haemolysin titer. These calculations were preferably performed automatically by a connected personal computer. The titers obtained by this method were identical with those obtained by the traditional tube test based on measurement of the absorbance of released haemoglobin. The standard deviations obtained for different haemolysin preparations were found to be 3.7-8%.

Automation↗
PubMed1987

Immunological relation between serum antibodies against pneumolysin and against streptolysin O.

The immunological relation between serum antibodies to pneumolysin and to streptolysin O was studied in patients with pneumococcal pneumonia (n = 40), patients with infections due to beta-haemolytic streptococci (n = 35), healthy human controls (n = 60) and in rabbits immunized with pneumolysin. There was no correlation between anti-pneumolysin and anti-streptolysin O titers (r = -0.279). The distribution of anti-pneumolysin titers in patients with high anti-streptolysin O titers did not differ from healthy controls. However, there was a tendency to increased or rising anti-streptolysin O titers in patients with pneumococcal infection. Antibodies obtained during pneumococcal infection might thus give false-positive reactions in the streptolysin O neutralization test. Serum antibodies to streptolysin O do not cross-react with pneumolysin in an ELISA. The pneumolysin ELISA for detection of pneumococcal disease will therefore not be disturbed by false-positive reactions due to antibodies directed against beta-haemolytic streptococci.

Animals↗
PubMed1987

Production and purification of Streptococcus pneumoniae hemolysin (pneumolysin).

Pneumolysin was found to be produced by 112 of 113 clinical isolates of Streptococcus pneumoniae and to be an intracellular hemolysin. A 10-liter-scale fermentor production and purification procedure was developed for this hemolysin. The culture was concentrated by filtration 10 times before centrifugation. The cellular content was purified by ion-exchange chromatography, covalent thiopropyl gel chromatography, and gel filtration. One batch operation resulted in 6 mg of highly purified pneumolysin, with a yield of 66% and a specific activity of 1,400,000 hemolytic units per mg. The pneumolysin had a molecular weight of 53,000 and an isoelectric point of 5.2. The purification method developed will be of value in future studies on this hemolysin.

Bacterial Proteins↗
PubMed1987

Diagnosis of pneumococcal pneumonia by enzyme-linked immunosorbent assay of antibodies to pneumococcal hemolysin (pneumolysin).

An enzyme-linked immunosorbent assay (ELISA) with a highly purified pneumolysin as the antigen was evaluated for serological diagnosis of pneumococcal pneumonia. One hundred four healthy controls were tested, and the specificity of the test was set to 95%. In samples from patients with bacteremic pneumococcal pneumonia, 82% (18 of 22) were positive, i.e., at least one serum sample had a titer above the upper normal limit or at least a twofold rise in antibody titers was noted. In nonbacteremic pneumococcal pneumonia, 45% (21 of 47) of samples were positive. All sera were negative for patients with pneumonia caused by Haemophilus influenzae, Legionella pneumophila, Chlamydia psittaci, and influenza A virus. However, in patients with a diagnosis of Mycoplasma pneumoniae infection, 8 of 25 (32%) samples were positive for antibodies to pneumolysin. All sera, including those from patients with mycoplasma infection, were negative to a protein control antigen by ELISA. Serum immunoglobulin G response to pneumolysin as measured by ELISA might thus be an aid in the laboratory diagnosis of pneumococcal pneumonia. This assay may also help to further elucidate the occurrence of dual infections with pneumococci.

Adolescent↗
PubMed1986

Effect of streptolysin S from Streptococcus pyogenes on contact sensitivity in mice.

Streptolysin S, a hemolytic toxin produced by strains of Streptococcus pyogenes, was examined for its effect on cellular immune reaction in mice. The toxin given intraperitoneally for six consecutive days did not influence intensiveness of delayed hypersensitivity to oxazolone which has been used as a model of cellular immune reaction. Streptolysin S injected subcutaneously, closely to lymph nodes directly involved in immune response, markedly suppressed delayed hypersensitivity. Significant inhibition of lymphocyte proliferation by streptolysin S was observed both in vivo as well as in vitro experiments.

Animals↗