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Biomedical subjects

K Katou

Publications and source records attributed to K Katou.

At least 19 recordsLinked to original sources
PubMed2001

Distribution of yieldin, a regulatory protein of the cell wall yield threshold, in etiolated cowpea seedlings.

We examined the distribution and the immunohistochemical localization of yieldin in etiolated cowpea seedlings with an anti-yieldin antibody. An immunoblotting analysis revealed that the yieldin was located in the aerial organs (plumule, epicotyl and hypocotyl) but not in the roots. The intensity of the yieldin signal in the hypocotyls was highest in the apical pre-elongation region (the hook region) and decreased toward the elongated mature base indicating that the yieldin disappeared with the ceasing of cell elongation. Tissue-print immunoblotting analysis using hypocotyls in different germination stages supports this view because the apical yieldin-rich regions, just beneath the cotyledonary node (the hook and rapidly elongating regions), acropetally migrated together with hypocotyl elongation. Immunohistochemical microscopy demonstrated that yieldin was localized in the cell walls of the cortex and epidermis of the germ axes. The present results are consistent with the view that yieldin participates in the regulation of cell wall yielding during elongation growth.

Cell Division↗
PubMed2000

Bombyx acid cysteine protease (BCP): hormonal regulation of biosynthesis and accumulation in the ovary.

We previously reported purification of the cysteine protease from Bombyx eggs (BCP) and the occurrence of the enzyme in various tissues of this insect. In the present paper, we present a detailed analysis of stage-specific changes in activity of BCP between the fourth larval instar and pupal-adult development. A synthetic fluorescent peptide, carbobenzoxy-L-Phenylalanyl-L-Arginine4-methylcoumaryl-7-amide (Z-Phe-Arg-MCA), was used to assay proteolytic activity. When tissue extracts were treated with anti-BCP serum before assay of enzyme activity, most activity towards Z-Phe-Arg-MCA was removed from the extracts. Therefore proteolytic activity in the present experiments is due mainly to BCP. We used Western blot and Northern blot analyses to determine tissue and stage specific expression of the enzyme. In the 5th larval fat body and hemolymph, BCP activity dramatically increased at the time of spinning, returning to the basal level before ecdysis. Northern blot analysis showed that a 1.5 kilobase mRNA which hybridizes to BCPcDNA suddenly appears during this period. Similar results were obtained in 4th instar fat body. In pupal hemolymph and fat body, low basal activity of BCP was detected early (day 0 to day 3 after pupal ecdysis), followed by a pronounced increase to a maximum six days after ecdysis, before returning to the basal level. In ovariectomized female pupae, a significant amount of proteolytic activity accumulated in hemolymph, suggesting that the enzyme is synthesized in the fat body and transferred into the ovary along with vitellogenin. BCP activity increased three days after injection of 20-hydroxyecdysone into ligated pupae. Furthermore, putative BCPmRNA appeared in the fat body within 24 hours after injection. This increase was completely blocked by the administration of cycloheximide. The results suggest that, BCP is synthesized in extraovarian tissues such as fat body and ovarian follicle cells and accumulates in the ovary, thus representing a new class of yolk protein.

Journal Article↗
PubMed1999

Gene expression during osteoclast-like cell formation induced by antifusion regulatory protein-1/CD98/4F2 monoclonal antibodies (MAbs): c-src is selectively induced by anti-FRP-1 MAb.

Human blood monocytes can differentiate into osteoclast-like cells when they are cultured in the presence of anti-FRP-1. Messenger (mRNA) expression of markers related to osteoclasts was analyzed during differentiation of osteoclasts from monocytes. As markers related to osteoclasts, we selected cathepsin-K, carbonic anhydrase (CA) II, vacuolar H(+)-ATPase (v-ATPase), vitronectin receptor (VNR), tartrate-resistant acid phosphatase (TRAP), osteopontin (OPN), galectin-3, c-src, c-fos, and c-fms. The mRNAs other than c-src mRNA were expressed in freshly isolated monocytes or monocytes incubated with control antibody or anti-FRP-1 monoclonal antibody (MAb) for 14 days. Of these mRNAs, cathepsin-K, CA II, v-ATPase, VNR, TRAP, OPN, and c-fms mRNAs were expressed at higher levels in the osteoclast-like cells than those in monocytes cultured with control antibody. On the other hand, galectin-3 mRNA was expressed at lower levels in the osteoclast-like cells, and there was no significant difference in c-fos mRNA expression between the monocytes cultured with control antibody and anti-FRP-1 MAb. c-src mRNA could not be detected in monocytes freshly isolated or incubated with control antibody. Surprisingly, expression of c-src mRNA was induced in monocytes by anti-FRP-1 MAb and was detectable as early as 3 h after anti-FRP-1 MAb treatment, indicating that c-src is selectively induced by anti-FRP-1 MAb treatment. Furthermore, the osteoclast-like cells expressed calcitonin receptor. Receptor activator of NF-kappaB (RANK) mRNA was detectable in freshly isolated monocytes or monocytes cultured with control antibody or anti-FRP-1 MAbs. Maximal expression of RANK was observed in osteoclast-like cells. On the other hand, no receptor activator of NF-KB ligand (RANKL) mRNA was detectable in any of the samples, suggesting that anti-FRP-1 mAb can induce osteoclast-like cells from blood monocytes without RANKL.

Antibodies, Monoclonal↗
PubMed1999

Restoring normal gait after limb salvage procedures in malignant bone tumours of the knee.

Eleven patients exhibiting decreased strength of knee extension following wide resection and prosthetic reconstruction for malignant bone tumors of the knee performed gait exercises with compensatory muscle training. Two patients whose knee extension strength was assessed as manual muscle test (MMT) grade 4 were able to develop a gait with double knee action and to maneuver stairs, step-by-step, due to compensation by the gluteus maximus, biceps femoris, and gastrocnemius muscles. Four patients whose knee extension strength was less than MMT grade 4, and whose ankle dorsal and plantar flexion was MMT grade 4 or higher, acquired the ability to go up and down stairs step-by-step, although their gait pattern was a knee-extended gait. Electromyographic studies demonstrated continuous discharges of the gluteus maximus, biceps femoris, and gastrocnemius muscles during the stance phase as compensation for decreased strength in knee extension.

Adolescent↗
PubMed1999

[Autoimmune hepatitis with drug-induced pneumonia due to Sho-saiko-to].

A 71-year-old woman was being treated with Sho-saiko-to for chronic hepatitis. On the 14th day, she complained of dyspnea; chest X-ray films and CT scans revealed ground-glass shadows in both lung fields. Under a suspected diagnosis of drug-induced pneumonia, Sho-saiko-to was discontinued and the patient was started on prednisolone. After several days, her laboratory data and chest X-ray findings were markedly improved. Cell analysis of bronchoalveolar lavage fluid disclosed an increase in the lymphocyte fraction and a depressed CD 4/CD 8 count. Lymphocyte stimulation tests of Sho-saiko-to and its ingredients, Ohgon and Hange, were positive in the case of peripheral lymphocytes, but not bronchoalveolar lavage lymphocytes. These findings yielded a diagnosis of drug-induced pneumonia caused by Sho-saiko-to. A histologic examination of needle biopsy specimens from the liver revealed severe lymphocytic infiltration into the tissues of Glisson's capsule and liver parenchyma, and mild infiltration by plasma cells into tissues surrounding Glisson's capsule. To our knowledge, this is the first case of autoimmune hepatitis with Sho-saiko-to-induced pneumonia to be reported.

Aged↗
PubMed1998

Multiple regression analysis of airway responsiveness in adult asthmatic patients.

The aim of this study was to determine which background variables exert the most influence on airway responsiveness in adult asthmatic patients. The relationships between airway response and background variables were investigated by multiple linear and logistic regression analysis in 97 asthmatic patients. Airway responsiveness was measured by the oscillation technique during methacholine inhalation challenge. The regression analyses revealed that modified Aas score "one of asthma severity score", was the major factor contributing to airway hyperreactivity. We conclude that the number of attacks during a previous year is the most important background factor related to airway hyperresponsiveness on a clinical basis.

Asthma↗
PubMed1995

[A case of idiopathic pulmonary arteritis with positive anti-myeloperoxidase antibodies].

A 38-year-old woman was referred to our hospital for severe pulmonary hypertension (pulmonary arterial pressure 63/36 mmHg). Digital subtraction angiography showed complete obstruction of the right main pulmonary artery and severe stenosis of the left main pulmonary artery. Although there were no symptoms or signs of systemic arterial lesions, the initial diagnosis was aortitis syndrome with pulmonary arterial involvement, and prednisolone therapy was started (60 mg/day). Pulmonary arterial pressure decreased to 53/12 mmHg. At a dosage of 20 mg/day, however, multiple nodular shadows were present on the X-ray film of the chest, but they disappeared after the dosage was increased. The level of anti-myeloperoxidase antibodies in her serum changed at almost the same time as multiple nodular shadows appeared on the chest X-ray film. Because anti-MPO antibodies have been never detected in patients with aortitis syndrome, polyangitis overlap syndrome was suspected. However, we found no evidence of systemic vasculitis; that is, vasculitis in other organs, including the kidney and the skin. Therefore, we made a diagnosis of idiopathic pulmonary arteritis with positive anti-MPO antibodies.

Adult↗
PubMed1995

[Wilson-Jones type angiosarcoma with marked response to intrapleural administration of interleukin-2].

A 64-year-old woman was admitted to our hospital with a hemothorax after one year of therapy for angiosarcoma that had arisen from the skin of the head. The hemothorax was believed to have resulted from metastasis of the angiosarcoma. Interleukin-2 (IL-2) at a dose of 20 x 10(4) U once daily was administered intrapleurally. Clinical improvement was first observed on the second day after the first dose of IL-2. Increases in natural and in lymphokine-activated killer activity of lymphocytes in pleural effusion were found on the eighth day. Starting on the 16th day of IL-2 therapy, no more fluid was drained, so administration of IL-2 was stopped. The clinical course indicated that the pleural effusion disappeared not because of pleurodesis but because of the anti-cancer effects of IL-2. There were no marked side effects, and intrapleural administration of IL-2 may be useful in patients with hemothorax due to metastasis of angiosarcoma.

Female↗
PubMed1994

[Relation of pulmonary vascular response to pressure-flow relationship during incremental exercise in patients with chronic obstructive pulmonary disease (COPD)].

Twenty-eight COPD patients underwent right heart catheterization while in clinically stable condition. Pulmonary vascular response to oxygen was evaluated by the percent change in pulmonary arteriolar resistance after 100% oxygen inhalation (% delta PAR), and its relation to the pressure-flow relationship during incremental exercise was assessed. Mean pulmonary arterial pressure (PPA) during exercise was plotted against the cardiac index (C.I.) from rest to maximal exercise in each patient. In most of the patients, the changes in PPA were nearly linear to the C.I. Therefore, a slope could be obtained from the regression equation in each patient. Patients were divided into two groups according to whether their % delta PAR was greater than 20 defined as a responder (RES), or less than five defined as a non-responder (N-RES). Seven out of 28 patients were RES, nine were N-RES, RES showed a higher %FEV1.0 level, C.I. and stroke volume index (S.I.) at maximal exercise, and a lower level of RV/TLC as well as slope. The slope correlated significantly with %DLCO (r = -0.724, p < 0.01), baseline PAR (r = 0.562, p < 0.01) and % delta PAR (r = -0.522, p < 0.01). These results suggest that the diminished pulmonary vascular bed, and the distensibility of pulmonary vessels, appear to contribute to the steepness of the slope and reduced % delta PAR in patients with COPD.

Administration, Inhalation↗
PubMed1993

[Synthesis of imidazo[1,2-a]pyridine derivatives and their reactions].

By the reaction of 2-benzoylmethylimidazoles (1a, b) with polarized olefins (2, 3) in the presence of K2CO3, the corresponding imidazo[1,2-a]pyridine derivatives (4, 5) were obtained. Methylsulfinylimidazopyridine derivative (7) obtained by the reaction of 5b with m-chloroperbenzoic acid (m-CPBA), easily underwent nucleophilic substitution to give the corresponding substituted compounds (8a--e). The substitution of chloroimidazopyridine derivative (11) with nucleophiles produced the corresponding substituted compounds (12a--d).

Alkenes↗
PubMed1993

[Characteristics of 201Tl myocardial SPECT and left ventriculography in patients with acute diagonal branch myocardial infarction].

Characteristics of 201Tl myocardial SPECT and ventriculography were studied in 13 patients with acute diagonal branch myocardial infarction. Rest 201Tl myocardial SPECT and left ventriculography were underwent in chronic phase. In 5 patients ECG changes in acute phase were not definite. In 6 patients it was difficult to identify the obstructed coronary artery with coronary angiography in acute phase. Mean value of maximum CPK was 854 (458-1,774) U/l. It seemed to be difficult to diagnose acute diagonal branch myocardial infarction with ECG and/or coronary angiography. In all patients defects were noted on 201Tl SPECT. Defects were small and noted in the central anterior wall and not in the septum. In 2 patients defects were noted at apex. In left ventriculography dyskinetic motion was noted in 10 patients; one patient showed apical aneurysm and 3 patients showed anterior wall aneurysm. In 3 patients anterior wall showed akinesis. It was concluded that 201Tl myocardial SPECT were useful for detecting diagonal branch lesion. In case of diagonal branch myocardial infarction size of defects were small and defects were not noted in the septum, however aneurysmal motion was frequently noted.

Adult↗
PubMed1993

[A case of lung cancer with myocardial metastasis with ECG suggestive of myocardial infarction].

A 64-year-old man was admitted to our hospital with complaints of chest pain on Sep. 26, 1991. ECG revealed myocardial infarction-like ST-elevation in II, III, aVF, V4, V5, and V6 but coronary angiography revealed no abnormal findings in the right and left coronary arteries, and no elevation of SGOT, LDH or CPK was found. Chest CT scan, UCG and chest MRI revealed a tumor invading into myocardium in the left cardiophrenic angle. Myocardial scintigraphy revealed a cold area in the inferior wall. Histologically, the tumor was squamous cell cancer. In spite of treatment, the patient died due to heart failure on Feb. 8, 1992. Myocardial metastasis showing a myocardial infarction-like ECG has been rarely reported.

Carcinoma, Squamous Cell↗
PubMed1993

[Scintigraphic appearance in stress 201Tl myocardial SPECT of midportion lesion in left anterior descending artery].

Scintigraphic appearance of left anterior descending artery (LAD) lesion in stress 201Tl myocardial SPECT was studied in 43 patients (pts) with midportion lesion in LAD confirmed by coronary angiography. Right margin of ischemic region in the basal short axial image was -36 +/- 17 degrees and left margin was -128 +/- 75 degrees. In 28 pts of 43 pts left margin lied beyond -120 degrees. From the results it was concluded that in most cases basal inferior wall was perfused by septal branches from LAD. In 6 pts with midportion lesion situated proximal to the first diagonal branch and in 12 pts with midportion lesion situated proximal to the second diagonal branch ischemic region in the apical portion was noted at both septum and anterolateral wall. Critical short axial image, in which right margin of ischemic region shifted from plus to minus area, was the 3.2nd short axial image in the former group and the 5.2nd in the latter group (p < 0.01). In 10 pts with midportion lesion situated distal to the large first diagonal branch, ischemic region was noted in septal wall and not in anterolateral wall. It was concluded that right margin of ischemic region in the basal coronal image was the most useful sign for predicting midportion lesion and left margin for dominance between LAD and right coronary artery and that apical anterolateral ischemia was sign for LAD lesion involving diagonal branch. Critical short axial image was useful for determining whether the lesion was situated proximal or distal to the first diagonal branch and whether the lesion was situated proximal to the second diagonal branch.

Coronary Vessels↗
PubMed1993

[Characteristics of plasma levels of myosin light chain I in patients under aorta-coronary bypass grafts].

After cardiac operation plasma level of ventricular myosin light chain I (LCI), determined with monoclonal antibodies to myosin fragment, were studied in 30 patients under bypass surgery to assess its possible role as a marker for perioperative myocardial injury. LCI rapidly increased and reached peak levels (15.8 +/- 9.0 gn/ml; T-peak) at 88 +/- 28 minutes after aortic declamp (T-Time). Then LCI rapidly decreased with fast half-time disappearance (1.1 +/- 0.7 hours). In 7 out of 30 patients LCI improved to normal range and MB reached maximum levels (28.8 +/- 11.9 U/L) at 6.3 +/- 2.3 hours after declamp. No abnormal change was noted in ECG and no new defects were noted in T1-201 myocardial images. In the remaining 23 patients LCI decreased to minimum levels (4.1 +/- 2.1 ng/ml; min-LCI) at 9.2 +/- 5 hours (min-Time) and then gradually increased to peak levels (8.4 +/- 5.8 ng/ml; M-peak, M-value) on day 3.3 +/- 1.0 (M-Time). MB reached maximum level (77.6 +/- 82.4 U/L, p < 0.1) at 3.4 +/- 1.4 hours (p < 0.001). Good correlation was noted among min-LCI, min-Time, M-value and summation of daily value of LCI (omega(LCI)) x min-LCI = 5.7-0.17 min-Time (r = -0.42) and M-value = 2.1 min-LCT(r=0.74) and omega(LCI) = -9.6 + 6.1 M-value (r = -0.96). From the rapid appearance of LCI peak levels after aortic declamp it was concluded that LCI, accumulated during aortic clamp, was washed out by aortic declamp.(ABSTRACT TRUNCATED AT 250 WORDS)

Aged↗
PubMed1993

[Pulmonary hemodynamic effects of cold pressor test in patients with chronic lung disease].

Cold Pressor Test (CPT) was applied to patients with chronic lung disease, including primary pulmonary hypertension, and the effects on pulmonary circulation were compared to those on systemic circulation. HR PPA, and TPR showed maximum values at the end of CPT (Time = 0) and recovered to baseline values 5 min after CPT. CO, however, did not change significantly after CPT. PPA and PAR showed maximum values at the end of CPT and then gradually decreased, but still were significantly higher than baseline values at 5 min after CPT. Nifedipine (NFP) reduced the maximum values of both TPR and PAR at the end of CPT. There was significant correlation between PPA change after CPT and baseline PPA value, and NFP decreased the slope of the regression line. These results indicate that cold stimulation induces both systemic and pulmonary vasoconstriction in patients with chronic lung disease, and this pulmonary vasoconstriction was correlated to the baseline pulmonary vascular tone. Moreover, nifedipine may reduce this cold stimulation-induced pulmonary vasoconstriction.

Chronic Disease↗
PubMed1992

[Interesting PYP, 201Tl, MIBG, AM and BMIPP myocardial SPECT images in a patient under successful reperfusion therapy].

Various types of radiopharmacons such as 201Tl, 99mTc-pyrophosphate(PYP), 123I-metaiodobenzyl-guanidine(MIBG), 111In-antimyosin Fab (AM) and 123I-beta-methyl iodophenyl pentadecanoic acid (BMIPP) were applied to a patient under successful reperfusion therapy. In the patient, elevated serum enzyme activity region in the subacute phase. Ten months after the ischemic event, AM uptake was noted at the region which maintained contractility. Two years after the ischemic event, depressed BMIPP uptake and delayed washout were noted at the apical region and the basal anteroseptal region. From these findings, the following conclusions were reached. Depressed 201Tl uptake was noted in the salvaged jeopardized myocardium. The lesions noted in the MIBG images showed depressed myocardial norepenephrine activity. This suggested that depressed sympathetic nervous function caused by severe ischemia persisted long after both myocardial perfusion and myocardial contractility had been restored. From abnormal AM uptake in the contractile myocardium myocardial cell damage, which permitted AM uptake, was persistent ten months after the ischemic event. Depressed BMIPP uptake and delayed washout suggested that abnormal fatty acid metabolism caused by severe ischemia was persistent. Severe ischemia caused various types of pathological states in the myocardium and radioisotope image was useful for studying these states.

3-Iodobenzylguanidine↗