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Biomedical subjects

K Katou

Publications and source records attributed to K Katou.

44 records · Page 3Linked to original sources
PubMed1983

[Placental tissue concentration of pregnancy-associated proteins in early and term pregnancies].

We examined the localization of five pregnancy-associated proteins such as pregnancy specific beta 1-glycoprotein (SP1), human chorionic gonadotropin (HCG), human placental lactogen (HPL), alpha 2-PA-glycoprotein (SP3) and pregnancy-associated plasma protein-A (PAPP-A) in the placentae of early and term pregnancies by means of the PAP method in immunohistochemical technology. It was found that the degree of staining of these proteins did not reflect their concentration in serum at each gestational age. Therefore, the concentrations of these proteins in the solution extracted from the placenta were measured. 1) Placental tissues were fixed with a 10% neutral formaldehyde solution and these were embedded into paraffin blocks. These specimens were used for the PAP method. The localizations of five pregnancy-associated proteins from 10 placentae at the gestational age of 7 to 10 weeks were compared with those at the gestation age of 38 to 41 weeks from 7 patients. The staining degree of SP1 in free villi was pale in both early and term pregnancies. HCG was stained deeply in early pregnancy but pale at term pregnancy. HPL was stained deeply at both gestational ages. Horseradish peroxidase reaction products from SP1, HCG and HPL were located chiefly in the syncytiotrophoblast. SP3 was not stained on the placental tissue. PAPP-A was stained to a greater extent in the cytotrophoblast in early pregnancy but pale in the syncytiotrophoblast at term pregnancy. 2) The concentrations of SP1, HCG, HPL, SP3 and PAPP-A were measured in the placental tissues in both early and term pregnancies. Placental tissues were obtained from 24 normal pregnancy patients aborted artificially at 8 to 11 weeks gestation and from 28 patients terminated by normal deliveries at 38 to 40 weeks gestation. The tissue was homogenated with 3 volumes of 1/2 PBS (0.005M phosphate-buffered 0.07M sodium chloride, pH 7.4). The supernatant was removed after centrifugation and stored at -20 degrees C until assayed. The concentration of SP1 was measured by single radial immunodiffusion. That of HCG was assayed by a directed Latex agglutination test (Gestate Slide Eiken). The concentrations of HPL, SP3 and PAPP-A were quantified by rocket immunoelectrophoresis. No significant difference in SP1 levels was shown between early and term pregnancies, and the SP1 level was 4.3 +/- 1.3 (mean +/- 1 S.D.) mg/dl at term pregnancy. The HCG level was 1,100 +/- 300 IU/ml in early pregnancy and at least 20-fold higher than that at term pregnancy.(ABSTRACT TRUNCATED AT 400 WORDS)

Chorionic Gonadotropin↗
PubMed1982

[Immunohistochemical study of SP1, HPL and HCG in chorionic tissue with early spontaneous and induced abortions].

Using an indirect enzyme-labeled antibody method, localization of SP1, HPL and HCG in spontaneous abortions (29 cases) was compared with those in induced abortions (39 cases). At the same time, histological changes were examined in the same specimens. 1) Histologically, the fibrinoid degeneration in cytotrophoblastic mass was more often found in spontaneous abortions than in induced abortions. Stromal fibrosis frequently existed in spontaneous abortions (6-10 weeks: 53%, 11-16 weeks: 93%). Stromal edema was rare in induced abortions, but was detected in spontaneous abortions (6-10 weeks: 13%, 11-16 weeks: 50%). These findings were increased with gestational age. 2) Horseradish peroxidase (HRP) reaction products from SP1, HPL and HCG located mainly in the syncytiotrophoblast. The intensity of HRP reaction was not always uniform in each villus. 8% in induced abortions and 59% in spontaneous abortions showed reaction with anti-HCG in some stromas in syncytiotrophoblast. Most of them also showed stromal fibrosis. In spontaneous abortions, the intensity of HRP reaction on HPL was relatively increased in comparison with that on HCG or SP1. In some severe degenerated specimens, SP1 and HPL were found in syncytiotrophoblast, but HCG seemed to be located on the outer part of the stroma adjacent to syncytiotrophoblast.

Abortion, Induced↗
PubMedDate not supplied

[Survey of studies on the role of play in child nursing].

In this survey, 103 papers submitted to several nursing societies and published in leading nursing journals are outlined and analyzed in terms of motives for studies, kinds of play enjoyed by the subjects and effects of play on members of ward staff, child patients and their family members. The articles are listed in chronological order so that the changes and trends in the studies in this field will be shown. Studies on play started and developed in the clinical field for better ward management and more effective treatment. However, effects of play on development of child patients gradually became the focus of the studies. In terms of the subjects' level of health, it used to be limited to chronically-hospitalized patients who were not behaviorally limited. The scope of the subjects has widened to include "every health level". It's now purported that every patient regardless of his/her health level should enjoy the benefits of play. Regarding kinds of play, indoor plays were initially predominant, but subsequently outdoor activities became more popular with the playing repertoire increasing. Infantile stage has been and still is most frequently discussed in the field, while other developmental stages are now attracting more attention than before.

Child↗