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Biomedical subjects

K Király

Publications and source records attributed to K Király.

At least 19 recordsLinked to original sources
PubMed1979

A new method for obtaining viable cells from dermal infiltrates. A study on 2,4 dinitrochlorobenzene induced contact dermatitis.

Experimental contact dermatitis has been induced in 2,4 dinitrochlorobenzene (DNCB) sensitized guinea pigs. The developing dermal infiltrate was excised and the infiltrating cells were obtained by mechanical extraction alone as well as by the combination with collagenase and elastase treatment. The most viable cells appeared in the elastase and mechanically extracted samples and the least in those subjected to mechanical treatment alone. The most cells in the enzyme-treated samples were present 24 h after re-exposure of the sensitized animals to DNCB consisting mainly of lymphocytes and of polymorphonuclear granulocytes. The optimum conditions for the action of enzymes including optimum duration of the treatment, buffer milieu, aspecific proteolytic effect on foreign substrate and action on T and B cell receptors have been elaborated. It was concluded that 80 min of collagenase treatment with gentle mechanical extraction under specified conditions does not affect any measurable immunologic properties of the liberated cells resulting in the second best yield. A comparison of these data with earlier reports and their significance is being discussed.

Animals↗
PubMed1979

Isomorphic skin reaction with DNCB in SLE and DLE.

DNCB sensitization was studied in 76 cases of systemic and 44 of discoid lupus erythematosus and in 101 immunosuppressed and tumor patients as controls, as well as in 40 contact-sensitized eczematous and 115 unselected patients. Sensitization of patients with discoid LE and that of mixed patients are of the same order, while it is lower in the systemic LE group. The highest incidence was found in the polysensitized positive control group; the lowest in the immunosuppressed and tumor patients. DNCB sensitization showed clear age dependency in the different groups. At the site of the DNCB exposure, isomorphic reactions were found in 43% of discoid and 25% of systemic LE patients. Histological and immunofluorescence studies supported the findings of the clinical picture, i. e. the appearance of isomorphic reactions at the site of the exposure cannot be related either to the intensity of the early reaction or with the developing intensity.

Adult↗
PubMed1979

Effect of KCN on the motility of Treponema pallidium.

Virulent Treponema pallidum has been shown to survive in KCN-containing artificial medium. Oxygen uptake being sensitive to cyanide, the observation indicates that treponemes do not have a cytochrome oxidase system. KCN had a killing effect only at a 15--30 mM final concentration. The data show that the Budapest strain of T. pallidum is an anaerobic organism.

Animals↗
PubMed1976

Objective measurement of topically applied corticosteroids.

The vasoconstriction caused by four topical corticosteroid preparations was compared according to subjective ranking and according to measurement by a tristimulus colourimeter (Momcolor). The measured results agreed well with visual evaluation. The colourimeter was proved to be measuring the degree of vasoconstriction. Among the preparations examined, the vasoconstriction effected by hydrocortisone 17-butyrate cream (Locoid) and by betamethasone dipropionate ointment (Diprosone) was stronger than that exerted by triamcinolone acetonide (Ftorocort) and fluocinolone acetonide (Flucinar). With the aid of colourimetric measurement of the vasoconstriction, differences could be demonstrated between clinically effective topical corticosteroid preparations. An open question remains, what the predictive significance is of the objectively measured vasoconstriction test, for clinical practice.

Administration, Topical↗
PubMed1976

Survival of T. pallidum under microaerobic conditions in cell and tissue cultures.

The survival of pathogenic T. pallidum was studied under microaerobic conditions (partial pressure of oxygen 7 mm Hg) with and without tissue cell cultures. As between one third and half of the germs were motile even after 96 hours of incubation, treponemes do not seem to be strictly anerobic as has been generally believed so far. Flocks containing enormous numbers of treponemes were observed in the nutrient medium. For their formation, human serum, testicular components from rabbits and modified Nelson's survival medium were essential. Although the problem of cultivation of T. pallidum remains to be solved the formation of flocks indicates a possible transient multiplication of treponemes in vitro.

Aerobiosis↗