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Biomedical subjects

K Kuczyńska

Publications and source records attributed to K Kuczyńska.

At least 19 recordsLinked to original sources
PubMed2003

Genetic influence on blood pressure and lipid parameters in a sample of Polish twins.

We studied 76 healthy monozygotic (MZ) and same-sex dizygotic (DZ) twin pairs (mean age 35 +/- 8 years, body mass index, BMI, 23.6 +/- 3.9 kg/m2) to determine genetic and environmental contributions to systolic (SBP) and diastolic (DBP) blood pressure, heart rate (HR) and serum lipids [total cholesterol (TC), low-density lipoprotein cholesterol (LDL-chol), high-density lipoprotein cholesterol (HDL-chol) and triglycerides (TG)I. SBP, DBP and HR were measured clinically and by ambulatory blood pressure monitoring (ABPM). Parameters of the genetic models for age-, sex- and BMI-adjusted data were estimated by model fitting and path analysis technique using LISREL 8. We found significant genetic effect on SBP and DBP for both clinical and ABP measurements, ranging from 37% for night-time ambulatory DBP to 79% for daytime ambulatory SBP. Estimates of genetic effects were higher for daytime than night-time ABP values, and higher for ambulatory 24-h SBP than office SBP measurements, with the reverse true for DBP. Significant genetic effect on HR ranged from 59% for office measurements to 69% for 24-h mean values. In summary, we also found genetic effect on TC, LDL-chol and HDL-chol with estimates ranging from 36% to 64%, but not on TG. Furthermore, a shared environmental component for TG was found, estimated at 36%. We showed significant genetic effect on both office and ambulatory BP and HR, with stronger genetic effect on daytime than night-time BP. We also found genetic effect on TC and lipoprotein fractions, but no significant genetic effect on TG. Environmental factors influencing serum TG, such as alcohol consumption, may explain the apparent lack of genetic effect in this healthy, non-obese population.

Adolescent↗
PubMed2001

[Influence of hormonal replacement therapy on bacterial vaginosis in the group of peri- and postmenopausal women].

The aim of the study was to evaluate microbiological biocenosis of the menopausal or postmenopausal women, and women having local hormonal therapy (Ovestin and Ortho-Gynest D). The study group consisted of 123 women (aged 45-72), who underwent clinical examination and microbiological evaluation of vaginal discharge. Miller's and Gramm's methods were used for slide staining and the culture method for growing microorganisms. Classical culture media were used as well as Rogosa culture medium and modified Weinstein medium for Lactobacillus culture in anaerobic conditions. In the microbiological studies before initiation of hormonal therapy, normal biocenosis of the vagina was found in 52 women (42.27%). The other part of the study group had abnormal vaginal biocenosis or had no microorganisms at all. During the hormonal therapy, the group of women with normal biocenosis increased to 53.65% and 62.29% after respectively 3 and 9 months of therapy. Hormonal therapy secured continuity of normal Lactobacillus morphotypes within perimenopausal period. Antimicrobial and hormonal therapies used together provided normalization of vaginal biocenosis in a shorter time. In the group of senile women, the recolonization of vaginal mucosa by Lactobacillus vaginalis was observed.

Adult↗
PubMed1997

Hemorrheological indices, catecholamines, neuropeptide Y and serotonin in patients with essential hypertension.

Hemorrheological and humoral abnormalities and excessive platelet activity can predict the development of cardiovascular complications in patients with essential hypertension. A study was conducted to assess the influence of gender on these factors and the interrelations between changes in hemorrheology and the sympatho-adrenal system in 54 patients (18 women, 36 men) with essential hypertension (aged 39.6 +/- 9.7 years) and 25 healthy volunteers (10 women, 15 men; aged 36.0 +/- 7.2 years). A decrease in erythrocyte deformability (p < 0.01) was found in the hypertensive men compared with the hypertensive women. Hematocrit (p < 0.01), blood viscosity at the shear rates of 0.3 s-1 (p < 0.01) and 6 s-1 (p < 0.01), plasma viscosity (p < 0.01), erythrocyte aggregation (p < 0.01), and neuropeptide Y (p < 0.02) concentrations were higher in the hypertensive men than in the hypertensive women. A positive correlation between blood fibrinogen and serotonin was found in the pooled hypertensive group and in the hypertensive men (p < 0.01) and between blood viscosity (shear rate 6 s-1) and neuropeptide Y in the pooled hypertensive group (p < 0.01). Neuropeptide Y correlated with filtration time of 1 mL blood in the hypertensive men (p < 0.05) and in the pooled normotensive group (p < 0.01) and with beta-thromboglobulin in the hypertensive women (p < 0.001). A positive correlation was also found in the hypertensive men between erythrocyte and platelet aggregation (p < 0.01) and between beta-thromboglobulin and adrenaline (p < 0.01). Hemorrheological and humoral abnormalities are more pronounced in men than in women with essential hypertension and may contribute to the increased incidence of cardiovascular events in men.

Adult↗
PubMed1996

[Evaluation of blood rheology indices in patients with pheochromocytoma].

Investigated group consisted of 28 patients (21 female and 7 male) with pheochromocytoma in mean age of 39.9 +/- 13 years, while control group comprised 25 healthy volunteers in mean age 36.0 +/- 7.2 years. Following parameters were determined: plasma and blood viscosity, erythrocyte and platelets aggregation, erythrocyte deformability, hematocrit, beta-thromboglobulin plasma concentration, plasma fibrinogen concentration, lipids concentration, noradrenaline, adrenaline, serotonin, neuropeptide Y plasma concentrations. Plasma fibrinogen and beta-thromboglobulin were increased in pheochromocytoma before surgery after pharmacological treatment and also after excision of the tumor. Whereas altered erythrocyte deformability before any treatment, only. Plasma neuropeptide Y concentration was higher in pheochromocytoma before surgery and after pharmacological treatment. There was no correlation between plasma catecholamines, plasma fibrinogen, beta-thromboglobulin, and erythrocyte deformability. Our results suggest that rheological abnormalities observed in patients with pheochromocytoma may contribute to vascular complications in this subjects. Sympathetic nervous system seems to influence indirectly observed rheological abnormalities.

Adult↗
PubMed1993

[Use of direct and serologic methods to detect Chlamydia trachomatis infections in cervix uteri of women].

Investigation included 254 women (18-75 years of age). In this number, 74 patients constituted a group with pathological changes in cervix uteri, while 180 women were seemingly healthy. The material was evaluated by a method of direct immunofluorescence (IF) with monoclonal antibody serum and a culture on McCoy cells. Antibodies in blood serum was detected by an immunoenzymatic test ELISA. Out of total number of 256 patients tested, elementary bodies of C. trachomatis were found in cylindrical cells of cervix uteri by the IF test in 54 (21.25%) of women. Analogical percentage of positive results was obtained by application of culture on McCoy cells. Positive serological results were found in 70 patients (27.55%). In group of 75 patients infected with C. trachomatis, 33 cases (44.59%) were confirmed by application of IF and cell culture, whereas positive serological results were found in 39 (52.70%) of investigated patients. In the group of seemingly healthy women in 11.60% elementary bodies of C. trachomatis were detected and in 17.22% of cases serological results were positive.

Adult↗
PubMed1993

[Biocenosis of vagina in women in light of microbiological examinations of vaginal content].

The study was aimed at microbiological evaluation of vaginal environment in 6810 women (19-75 years of age). Diagnostic procedure was applied according to accepted scheme, including basic and selected methods. Evaluation was performed by investigation of fresh (Miller's method) and fixed (Gram method) smears of vaginal content of pregnant women, women in childbirth and in women not pregnant, as well in the menopause and the old age. Microbiological pictures of vaginal content were classified according to modified procedure of Manu Af Heurlin. At the same time, cultures were carried on enriched and differentiating media in aerobic and anaerobic conditions. Microbiological investigations of vaginal content permitted for establishment of physiological status of vaginal environment or normal biocenosis of vagina in 3910 (57.4%) of tested patients. Disturbed biocenosis was present in 2900 (42.6%) of women and this included very serious disturbances of biocenosis in 1077 patients. In disturbed biocenosis of vagina, sexually transmissible microorganisms were main cause.

Adult↗
PubMed1990

[Trichomonas vaginitis at different life stages of women].

Using of fresh slides and culture T. vaginalis was found in 1094 persons (3.20%). It has been seen the morphological differentiation of T. vaginalis connected with the age, physiological state of macroorganisms and the clinic picture of trichomoniasis. In vivo, the spheroidal as well as ameboid forms of T. vaginalis were observed. The latter ones, characteristic in acute trichomoniasis, were often seen in pregnant women. On the other hand, nonmobile round-shaped forms of T. vaginalis occurred in vaginal contents of girls, women in child-bed and old (5 years after menopause) women. It seems, that morphologically + variability of T. vaginalis depends on changes of specific environment of human vagina, which is very sensitive to hormonal+ responses. It may be considered as adaptation of parasite to different biological conditions of vaginal environment.

Adolescent↗
PubMed1990

Platelet activity, prostacycline metabolite, plasma lipids and sympathoadrenal activity in patients with borderline hypertension and a positive family history of hypertension.

The study included 30 patients with borderline essential hypertension (HPT) (21 with a positive family history of hypertension, mean age 24.6 years, 9 with a negative family history, mean age 27.2 years) and 10 normotensive controls (mean age 27.5 years). In all of them 24-hour urinary noradrenaline (NA) and adrenaline (A) excretion was assayed. Blood levels of NA, A and dopamine, the prostacycline metabolite 6-keto-PGF1 alpha, beta-thromboglobulin, cholesterol, triglycerides and HDL cholesterol were measured, LDL cholesterol was calculated according to the Friedewald equation. Besides, lecithin cholesterol acyltransferase activity was assayed. Patients with HPT and a positive family history had elevated sympathetic and platelet activity and diminished 6-keto-PGF1 alpha blood levels. Their HDL cholesterol level was significantly lower than that of healthy controls. In patients with HPT and a positive family history of HPT the atherogenic index (total cholesterol to HDL cholesterol ratio) was highest, but did not differ significantly from that in other groups. The assessment of the examined humoral factors indicates that patients with borderline HPT with genetic predisposition to high blood pressure have a humoral profile different from that of patients without genetic predisposition. These findings suggest the importance of genetic factors in the development of essential HPT.

6-Ketoprostaglandin F1 alpha↗