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K Kuki

Publications and source records attributed to K Kuki.

29 records · Page 2Linked to original sources
PubMed1988

Immunohistological studies on tonsils of recurrent tonsillitis and tonsils with focal infections.

In this study, two experiments were made to investigate the immunological pathogenesis of PPP, a skin disease of so-called focal infections. One was the immunohistological study on tonsils with PPP and recurrent tonsillitis. In the investigation, monoclonal antibodies against T lymphocytes and anti-immunoglobulin antibodies were used to reveal the distribution of T and B cell subsets on frozen sections of tonsils. But no difference between tonsils with focal infection and recurrent tonsillitis was observed in the distribution of T and B cell subsets. The other was the identification of a common antigen between tonsillar epithelium of PPP and hand-sole skin by use of one monoclonal antibody against PPP-tonsillar epithelium, which was made in our laboratory. Monoclonal antibody ATE-3 was in IgM, and recognized about 35 kilo dalton molecular weight protein of a tonsillar epithelium of PPP. This antibody reacted with the basal layer cells of the tonsillar epithelium and the suprabasilar cells of plantar skins of PPP. But ATE-3 did not react with normal tonsillar epithelium and normal plantar skin.

Adult↗
PubMed1987

[A case of B cell malignant lymphoma with in the liver and brain metastasis in Hashimoto's disease].

We have reported a case of malignant lymphoma in associated with Hashimoto's disease arising in the right lobe of thyroid of a 57-year-old woman. The pre-operative aspirated biopsy was valuable for diagnosis Immuno-histochemical observations revealed the localization of anti B cell antibody in the cell surface of lymphoma cells and anti thyroglobulin antibody in intactly follicular epithelium within the lymphoma tissue. This lymphoma was diagnosed as diffuse, medium cell and B cell type. Radiation and chemotherapy were performed for multiple metastasis of liver and brain, but this case had died of DIC and diffuse hyaline membrane disease of lung.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1987

[Evaluation of the prognostic factor in human gastric cancer with special reference to the relationship between proliferation and carcinoembryonic antigen production of the cancer cell].

The biological property of human gastric cancer was studied by morphological and immunohistochemical observations. The cytoplasmic mucin of cancer cells was mainly neurtromucin in the mucosa. However, acid mucin was increased in the cancer cells of signet-ring cell carcinoma with submucosal infiltration. Immunohistochemically, CEA was localized in the plasma membrane of matured signet-ring cells and the cytoplasm of immature cancer cells. On electron microscopic observation, the cytoplasmic organellae were prominent in the immature cells which were dominant in submucosal infiltrating region. In vitro, the cancer cell line established from signet-ring cell carcinoma revealed that CEA production was associated with cell growth property showing 3H-TdR and BrdU uptake. This results may suggest that CEA production was an important indicator for the decision of prognosis in signet-ring cell carcinoma.

Adenocarcinoma, Mucinous↗
PubMed1984

Immunohistological studies on tonsils with recurrent tonsillitis and focus-tonsil with pustulosis palmaris et plantaris.

By use of monoclonal antibodies and anti-immunoglobulin antibodies, the distributions of 15 adult tonsils with recurrent inflammation and palmoplantar pustulosis as focus-tonsil were investigated. The avidin-biotin-peroxidase complex (ABC) method was utilized throughout the experiment. Monoclonal antibodies of the Leu and OKT series revealed the location of T and B cell subsets characteristic in the tonsils with chronic inflammation and the focus tonsils with pustulosis palmaris et plantaris (PPP). In vitro sensitization of tonsil tissues was performed with crude antigen from tonsillar epithelium. Tonsil tissues with PPP demonstrated a significant increase in S-IgD-bearing lymphocytes and esterase-positive cells in the mantle zones of follicles, although the tissues of recurrent tonsillitis showed no such effects. It was speculated that tonsils with focal infections are usually standby for the production of antibodies against tonsillar epithelial antigens.

Adult↗
PubMedDate not supplied

Neurochemical and pathological alterations following infusion of leupeptin, a protease inhibitor, into the rat brain.

It is known that proteases participate in cellular protein turnover and eliminate abnormal and potentially toxic proteins. Disturbed proteolysis may be responsible for generating the pathological features of some neurodegenerative disorders. Alzheimer disease, for instance, is the most common neurodegenerative disorder and a condition in which proteins of the cell membrane and cytoskeleton are abnormally processed and accumulated in the brain. It is of interest to investigate the effect of protease inhibitors on neurons and neurotransmitter systems in the brain. We examined neurochemical and morphological neuronal changes in the rat brain following long-term intracerebroventricular infusion of leupeptin, a potent calcium-activated protease (calpain) inhibitor. Leupeptin (5 mg) was infused into the lateral ventricle using an osmotic minipump for 14 days. We found a significant reduction of regional choline acetyltransferase activities in the hippocampus, and of somatostatin concentrations in the hypothalamus and entorhinal cortex. Moreover, leupeptin caused a wide-spread, highly significant decrease in neuropeptide-Y concentrations. Leupeptin infusion produced severe degeneration of neuronal processes in both axons and dendrites, and accumulation of electron-dense bodies in the hippocampus. The results indicate that long-term intracerebroventricular infusion of leupeptin in the rat produces neurochemical and morphological changes resembling those of some neurodegenerative disease and aging. Abnormal proteolysis caused by either reduced protease or enhanced protease inhibitor activities might play an important role in these conditions.

Animals↗
PubMedDate not supplied

HLA-DR antigen expression in tonsillar epithelium. With special reference to focal infection.

Palmoplantar pustulosis (PPP) has long been regarded as a tonsillar focal infection because tonsillectomy is so effective in its treatment. Immune mechanisms of focal infection have been discussed mainly with regard to humoral immunity as an autoimmune disease. The present immunological study demonstrates the expression of HLA-DR antigen in tonsillar epithelium. The patterns of the HLA-DR antigen appearance in the epithelium were categorized into 4 types, and the intensity was compared between hypertrophic tonsils, recurrent tonsillitis, and tonsils with PPP. In addition, paired fluorescent staining, a combination of CD3 and anti-HLA-DR antibodies, was performed. The activity of intraepithelial T-lymphocytes was determined as an index of the HLA-DR antigen expression. In an age matching study, HLA-DR antigen appeared in high intensity in tonsils with PPP. A close correlation was found between the appearance of HLA-DR antigens and activation of infiltrating T-lymphocytes. These findings suggest that the tonsillar epithelium plays an important role in focal infection.

Child↗