Biomedical subjects

K Kunimi

Publications and source records attributed to K Kunimi.

44 records · Page 3Linked to original sources

PubMed1991

Recessive genetic mechanisms in the oncogenesis of prostatic carcinoma.

A detailed deletion mapping of a series of human prostatic carcinomas, using restriction fragment length polymorphism (RFLP) analysis of all chromosomes, showed allelic losses on individual chromosomes at variable frequencies. Allelic losses occurred on chromosomal arms 8p, 10pq, 16q and 18q in more than 30% of the cases. Losses of genetic information from one or two of the chromosomes 8, 10, or 16 were always present in tumors showing allelic losses, indicating that genes on these chromosomes have a central role in prostatic cancer. A more extensive study of these chromosomes was thus carried out and showed the highest frequency of allelic deletions to occur on the short arm of chromosome 8 (65%) (where the minimally deleted region was between the PLAT locus and pter). The long arm of chromosome 16 had allelic deletions in 56% of informative cases, with three different break points (the most distal being between D16S4 and D16S7). Chromosome 10 exhibited a complex deletion pattern, showing allelic losses from both the short (p) and the long (q) arms, evidence of non-reciprocal translocations of the q arm, and monosomy in some cases. Our data indicate that tumor suppressor genes involved in the oncogenesis of prostatic carcinoma may be localized between 8pter and the PLAT locus and that additional/alternative tumor suppressor gamma are likely to be localized on chromosome 10 and on the long arm of chromosome 16. More aggressive tumors were accompanied by a high or frequency of allelic losses.

Alleles↗

PubMed1990

A gene near the D3F15S2 site on 3p is expressed in normal human kidney but not or only at a severely reduced level in 11 of 15 primary renal cell carcinomas (RCC).

Renal Cell Carcinoma (RCC) has been associated with the loss of heterozygosity at several loci on the short arm of chromosome 3 (3p). We have previously found that one of these loci, D3F15S2 (pH3H2) was lost in 76% of the tumor cells derived from heterozygous donors (Kovacs, G., Erlandsson, R., Boldog, F., Ingvarrsson, S., Müller-Brechlin, R., Klein, G. & Sümegi, J. (1988), Proc. Natl. Acad. Sci., 85, 1571-5). More recently we have identified a putative CpG island in the vicinity of D3F15S2, suggesting that DNA sequences in or around this site may have coding potential (Boldog, F., Erlandsson, R., Klein, G. & Sümegi, J. (1989). Cancer Genet. Cytogenet., 42, 295-306). The screening of a human placenta cDNA library with DNA probes derived from D3F15S2 has led to the isolation of several cDNA clones. They identified a 2.9 Kb long message in human placenta and kidney. In total RNA from 11 of 15 primary RCCs the gene expression was reduced to less than 20% compared to eight normal kidneys. This low level of expression may be due to contaminating normal tissue. In the remaining 4 tumors the expression varied from 24-51% compared to normal kidney. To facilitate reference, the gene was provisionally designated as 'RIK'. It was expressed in the HEK 293, one osteosarcoma (HOS), two carcinoma (COLO320 and QDMT), and three Burkitt lymphoma lines (BL2, BL29 and BL31). It was not expressed in one Burkitt lymphoma (DG75) and two EBV transformed lymphoblastoid cell lines (LCL) (NAD-20 and Cherry).

Carcinoma, Renal Cell↗

PubMed1989

[A case of lupus cystitis].

A case of lupus cystitis in a 23-year-old male is reported. The patient began to complain of diarrhea and vomiting in October, 1985. When the diagnosis of systemic lupus erythematosus (SLE) was established at the Department of Internal Medicine in our hospital, he was referred to our clinic for examination of pollakisuria on November 22. DIP revealed a loss of bladder distensibility, and bilateral hydronephrosis and hydroureter. Transurethral cold cup biopsies revealed subcutaneous edema. A diagnosis of lupus cystitis was made and he was treated with steroids, which resulted in symptomatic and radiographic improvement.

Adult↗

PubMed1989

[Adrenocortical carcinoma with Cushing syndrome: report of a case].

We report a 45-year-old female with left adrenocortical carcinoma resulting in Cushing syndrome. She visited the 3rd Department of Internal Medicine, Kanazawa University Hospital with complaints of moon face, amenorrhea and hypertension. A diagnosis of left adrenal tumor with Cushing syndrome was made and she was transferred to our clinic. Left thoracoabdominal adrenalectomy was performed. The histologic report was compatible with adrenocortical carcinoma with no invasion into adjunctive tissues. She is now on endocrinologic study and is being administered 1,1-dichloro-2-[o-chlorophenyl]-2[p-chlorophenyl] ethane. There is no evidence of local recurrence or remote metastasis.

Adrenal Cortex Neoplasms↗

PubMed1989

[A case of bilateral renal angiomyolipoma diagnosed by fine needle biopsies].

We describe a 37-year-old female with bilateral renal angiomyolipoma. She visited the Emergency Department at our hospital because of right severe flank pain. Computed tomography (CT) disclosed a bilateral renal tumors with fat components and high density lesions compatible with a hematoma. Conservative treatment was started, resulting in the improvement in laboratory findings and symptoms. Needle biopsies were performed under the guidance of ultrasonography. The histology was renal angiomyolipoma. She has been receiving a follow-up study at our out-patient clinic with an uneventful course.

Adult↗

PubMed1987

[Complete ureteric duplication associated with ureterocele: two cases report].

Herein we report two cases of complete ureteric duplication associated with ureterocele. A 4-month-old girl with non-visualizing left kidney was hospitalized with complaints of cloudy urine and fever. An antegrade left pyelogram revealed a completely duplicated collecting system; a duplex tortuous ureter, and round radiolucent filling defect in the bladder. Under the diagnosis of completely duplicated ureter associated with the upper pole ureterocele, ureterocystoneostomy was performed. A circumferential incision was made around the base of the ureterocele and the Politano-Leadbetter technique was employed. Her postoperative course was uneventful. However, an excretory urogram showed a mild hydronephrotic upper pole. A 1-year and 4-month-old girl with the history of repeated urinary tract infections and fever attacks was admitted. The excretory urogram showed a left hydronephrotic lower pole with a "drooping flower" and no opacification of the upper pole. A radiolucent filling defect was seen in the bladder. A left antegrade pyelogram demonstrated the massively dilated upper pole with a hydroureter terminating in the ureterocele. Left heminephrectomy with partial ureterectomy was performed and left hydronephrosis and hydroureter with an atrophic kidney were seen. The postoperative course was uneventful.

Female↗

PubMed1986

[Photodynamic therapy of superficial bladder tumors].

Photodynamic therapy (PDT), using hematoporphyrin derivative (HPD) and the red light (wavelength 630 nm) of an argon-dye laser as the source of excitation energy was performed on 46 patients with superficial bladder tumors. Two methods of laser irradiation, (1) focal PDT using a 400 micron quartz fiber through a cystourethroscope in 22 patients with superficial bladder tumors and (2) whole bladder wall total PDT using a motor-driven laser light scattering device in 24 patients with multifocal carcinoma in situ and/or dysplasia of bladder mucosa associated with multicentric concurrent superficial tumors, were used. The patients in (2) had been referred for total cystectomy, and 19 of these 24 patients had a history of several transurethral resections, hyperthermia and/or instillation therapy. HPD 2-4 mg/kg was i.v. injected 48 to 72 hours before PDT. Judging from the results of 60 protrusions treated by focal PDT, the light power should be 200 mW/cm2 for 5-10 minutes or more and the total light energy should be 100 J/cm2 or more in tumors up to 2 cm in size. With focal PDT, 4 of the 22 patients had no recurrence with the mean tumor free time of 20.8 months. In 6 of the 24 patients treated with total PDT using 10, 20 or 30 J/cm2 of light energy, there was no recurrence with a mean tumor-free time of 7.5 months and there was no significant relationship between the recurrence rate and total light energy used.

Adult↗

PubMedDate not supplied

Fluorescence spectra from human semen and their relationship with sperm parameters.

Fluorescence spectra of human semen, seminal plasma, and spermatozoa were investigated. A total of 51 semen samples were obtained from 45 men, including 40 suspected subfertile men and 5 normal volunteers. Fluorescence spectra excited at 488 nm for each component in 10 mM phosphate-buffered saline (PBS) were scanned in a range of 500 to 700 nm. Under these conditions, emission peaks from each component were observed at 622 nm. The intensity of emission peaks from spermatozoa at 622 nm correlated strongly with the concentration of spermatozoa (r = .837, p = .0001). Sperm motility also correlated significantly with fluorescence intensity emission peaks from spermatozoa (r = .369, p = .019) and semen plasma (r = .356, p = .024). To clarify the origin of emission peak of 622 nm with 488 nm excitation, some components in seminal plasma were dissolved in PBS and the fluorescence spectra were measured. The standard single solutions in PBS, such as citric acid, zinc, calcium, and riboflavin (vitamin B2), did not yield an emission peak of 622 nm with 488 nm excitation. However, blood plasmas and albumin revealed similar fluorescence peaks at 622 nm. Thus, the fluorescence spectra may originate from some protein complex.

Adult↗