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Biomedical subjects

K L Baker

Publications and source records attributed to K L Baker.

At least 19 recordsLinked to original sources

Tomographic reconstruction of high-energy-density plasmas with picosecond temporal resolution.

Three-dimensional reconstruction of the electron density in a plasma can be obtained by passing multiple beams at different field angles simultaneously through a plasma and performing a tomographic reconstruction of the measured field-dependent phase profiles. A relatively simple experimental setup is proposed and simulations are carried out to verify the technique. The plasma distribution is modeled as a discrete number of phase screens, and a Zernike polynomial representation of the phase screens is used to reconstruct the plasma profile.

Journal Article↗

A single-shot pixellated phase-shifting interferometer utilizing a liquid-crystal spatial light modulator.

We introduce a novel phase-shifting pixellated interferometer based on a liquid-crystal spatial light modulator and simulate the expected performance. The phase-shifted frames are captured simultaneously, which reduces problems arising from vibrations and air turbulence. The liquid-crystal spatial light modulator is flexible and can be configured to provide a large number of phase-shift levels and geometries to reduce measurement error.

Journal Article↗

High-speed horizontal-path atmospheric turbulence correction with a large-actuator-number microelectromechanical system spatial light modulator in an interferometric phase-conjugation engine.

Results of atmospheric propagation for a high-speed, large-actuator-number adaptive optics system are presented. The system uses a microelectromechanical system- (MEMS-) based spatial light modulator correction device with 1024 actuators. Tests over a 1.35-km path achieved correction speeds in excess of 800 Hz and Strehl ratios close to 0.5. The wave-front sensor was based on a quadrature interferometer that directly measures phase. This technique does not require global wave-front reconstruction, making it relatively insensitive to scintillation and phase residues. The results demonstrate the potential of large-actuator-number MEMS-based spatial light modulators to replace conventional deformable mirrors.

Journal Article↗

Performance of a phase-conjugate engine implementing a finite-bit phase correction.

The achievable Strehl ratio when a finite-bit correction to an aberrated wave front is implemented is examined. The phase-conjugate engine used to measure the aberrated wave front consists of a quadrature interferometric wave-front sensor, a liquid-crystal spatial light modulator, and computer hardware-software to calculate and apply the correction. A finite-bit approximation to the conjugate phase is calculated and applied to the spatial light modulator to remove the aberrations from the optical beam. The experimentally determined Strehl ratio of the corrected beam is compared with analytical expressions for the expected Strehl ratio and shown to be in good agreement with those predictions.

Journal Article↗

Electron density characterization by use of a broadband x-ray-compatible wave-front sensor.

The use of a Hartmann wave-front sensor to accurately measure the line-integrated electron density gradients formed in laser-produced and z-pinch plasma experiments is examined. This wave-front sensor may be used with a soft-x-ray laser as well as with incoherent line emission at multikilovolt x-ray energies. This diagnostic is significantly easier to use than interferometery and moiré deflectometry, both of which have been demonstrated with soft-x-ray lasers. This scheme is experimentally demonstrated in the visible region by use of a Shack-Hartmann wave-front sensor and a liquid-crystal spatial light modulator to simulate a phase profile that could occur when an x-ray probe passes through a plasma. The merits of using a Hartmann sensor include a wide dynamic range, broadband or low-coherence-length light capability, high x-ray efficiency, two-dimensional gradient determination, multiplexing capability, and experimental simplicity. Hartmann sensors could also be utilized for wavelength testing of extreme-ultraviolet lithography components and x-ray phase imaging of biological specimens.

Journal Article↗

Polycyclic aromatic hydrocarbons in sediments, mussels and crustacea around a former gasworks site in Shoreham-by-Sea, UK.

Concentrations of polycyclic aromatic hydrocarbons have been determined in sediments, mussels and crustacea in the vicinity of a former gasworks site by Shoreham Harbour, UK. Very high concentrations of PAH were found in the substrate, an ash-like material deposited on the former gasworks site, which exhibited a profile consistent with the major source of contamination being coal or coke tar produced during the period of gas production at the site. Elevated PAH concentrations were also found in mussels both from the beach below the former gasworks site, and from sites further to the east in Portslade and Hove. The significance of these concentrations were assessed using an approach which involved the calculation of benzo[a]pyrene equivalent conoentrations (BaPEs), summing concentrations of individual PAH on the basis of their comparative potency as carcinogens. BaPE ranged from values of, or close to, zero for crustacea, to 336 microg kg(-1) wet weight in mussels from Southwick Beach. The contaminated mussels are not exploited commercially but may be taken by casual gatherers, and notices have been posted to warn potential consumers.

Animals↗

Alternative mechanism for omega(0)/2 emission in laser-produced plasmas.

Several models have been proposed to explain the broad spectral features characteristic of omega(0)/2 emission observed in laser-produced plasmas. In this article, the electromagnetic decay instability is examined as an alternative explanation for this emission. It is shown that the electromagnetic decay instability is able to explain some of the spectral features observed from laser-produced plasmas. In addition, the electromagnetic decay instability is consistent with two other features observed in experiments: the efficient generation of electromagnetic energy and the discrepancy in the levels of emission between the omega(0)/2 emission and the 3omega(0)/2 emission.

Journal Article↗

Evaluating frequency proximity in stream segregation.

Consecutive sounds of similar structure that are close in frequency or pitch are more likely to be perceived as part of the same sequence than those at greater frequency separations. The principle of grouping into such perceptual sequences, or auditory streams, is known as frequency proximity. However, the metric by which one frequency difference is judged to be greater or less than another in complex auditory scenes is not yet known. Two experiments explored the metric for frequency proximity. We presented repeating three-tone stimulus patterns at a rate where they are normally heard as two streams, one containing the highest tone and one containing the lowest. The middle tone joined one stream or the other depending on its frequency. Subjects reported the perceived allocation of the variable tone by responding on a 5-point scale. The frequency at which either of these two percepts was equally probable was found to be lower than a logarithmic midpoint or the midpoints on a cochlear map or the Mel scale; that is, it was unlike metrics arrived at by direct comparisons of tones. Further, the midpoint for high and low tones presented synchronously was lower than that for the same tones presented sequentially, demonstrating that in addition to a proximity factor, some additional factor or factors must operate differently when the lower and upper fixed tones are, or are not, presented simultaneously.

Adolescent↗

Discharge characteristics of laryngeal single motor units during phonation in young and older adults and in persons with parkinson disease.

Discharge characteristics of laryngeal single motor units during phonation in young and older adults, and in persons with Parkinson disease. The rate and variability of the firing of single motor units in the laryngeal muscles of young and older nondisordered humans and people with idiopathic Parkinson disease (IPD) were determined during steady phonation and other laryngeal behaviors. Typical firing rates during phonation were approximately 24 s/s. The highest rate observed, during a cough, was 50 s/s. Decreases in the rate and increases in the variability of motor unit firing were observed in the thyroarytenoid muscle of older and IPD male subjects but not female subjects. These gender-specific age-related changes may relate to differential effects of aging on the male and female voice characteristics. The range and typical firing rates of laryngeal motor units were similar to those reported for other human skeletal muscles, so we conclude that human laryngeal muscles are probably no faster, in terms of their contraction speed, than other human skeletal muscles. Interspike interval (ISI) variability during steady phonation was quite low, however, with average CV of approximately 10%, with a range of 5 to 30%. These values appear to be lower than typical values of the CV of firing reported in three studies of limb muscles of humans. We suggest therefore that low ISI variability is a special although not unique property of laryngeal muscles compared with other muscles of the body. This conceivably could be the result of less synaptic "noise" in the laryngeal motoneurons, perhaps as a result of suppression of local reflex inputs to these motoneurons during phonation.

Action Potentials↗

Postischemic administration of AK275, a calpain inhibitor, provides substantial protection against focal ischemic brain damage.

Experiments were conducted to determine whether a potent, reversible calpain inhibitor could reduce the cortical ischemic brain damage associated with focal ischemia in the rat. AK275 (Z-Leu-Abu-CONH-CH2CH3), the active isomer of the diastereomeric mixture, CX275, was employed in conjunction with a novel method of perfusing drug directly onto the infarcted cortical surface. This protocol reduced or eliminated numerous, nonspecific pharmacokinetic, hemodynamic, and other potentially confounding variables that might complicate interpretation of any drug effect. Focal ischemia was induced using a variation of the middle cerebral artery occlusion method. These studies demonstrated a reliable and robust neuroprotective effect of AK275 over the concentration range of 10 to 200 microM (perfused supracortically at 4 microliters/h for 21 h). Moreover, a 75% reduction in infarct volume was observed when initiation of drug treatment was delayed for 3 h postocclusion. Our data further support an important role of calpain in ischemia-induced neuropathology and suggest that calpain inhibitors may provide a unique and potentially powerful means of treating stroke and other ischemic brain incidents.

Animals↗

Calpain inhibitor AK295 protects neurons from focal brain ischemia. Effects of postocclusion intra-arterial administration.

BACKGROUND AND PURPOSE: This research was performed to determine whether a selective inhibitor of the calcium-dependent protease, calpain, could reduce ischemia-associated brain damage when peripherally administered after a vascular occlusion. METHODS: A variation of the rat middle cerebral artery occlusion model was used. A range of doses of AK295 (a novel calpain inhibitor synthesized for this purpose) was continuously infused through the internal carotid artery, beginning 1.25 hours from the initiation of the occlusion. Rats were killed at 21 hours, and the infarct volume was quantified. RESULTS: Postocclusion (1.25-hour) infusion of the calpain inhibitor AK295 elicited a dose-dependent neuroprotective effect after focal ischemia. The highest dose tested (3 mg/kg per hour) afforded the maximum effect, illustrated by a 32% reduction in infarct volume 21 hours after the ischemia (vehicle, 81.7 +/- 4.7 mm3; AK295, 54.9 +/- 6.9 mm3; P < .007). CONCLUSIONS: These data provide the first evidence that a peripherally administered calpain inhibitor can protect against ischemic brain damage. They offer further support for an important role of calpain proteolysis in the brain degeneration associated with cerebral ischemic events and suggest that selective calpain inhibitors provide a rational, novel, and viable means of treating such neurodegenerative problems.

Animals↗

Perceived and actual level of knowledge of diabetes mellitus among nurses.

The purpose of this study was to survey staff nurses on their perceived and actual level of knowledge of diabetes mellitus. A convenience sample of 184 professional staff nurses from both inpatient and outpatient settings of a large research-teaching hospital was surveyed. The Diabetes Self-Report Tool (Cronbach's alpha = .91) was used to assess staff nurses' perceptions of knowledge of diabetes mellitus. The Diabetes: Basic Knowledge Test (DBKT; Cronbach's alpha = .79) was used to measure the actual level of knowledge of diabetes mellitus. The data were analyzed with Pearson's correlation coefficients. A moderately low negative correlation (r = -.36, P less than .001) indicated that the staff nurses' perceived knowledge of diabetes mellitus was inversely related to actual knowledge. Subjects were found to have a mean score of 64% on the DBKT. Study findings raise questions as to the adequacy of staff nurse knowledge of diabetes and the ability of staff nurses to assess themselves for knowledge deficits.

Diabetes Mellitus↗

Frictional changes in force values caused by saliva substitution.

The objective of this study was to determine the magnitude of frictional force changes between several sizes of stainless steel orthodontic wires (0.018 inch round, 0.020 inch round, and 0.018 X 0.025 inch rectangular) and an edgewise bracket (0.022 X 0.028 inch slot) when an artificial saliva medium was introduced. The wires were secured in the bracket slots with a 0.010 inch polyurethane ligatures. The force values in the saliva substitute medium were compared with those produced in a dry control and in glycerin. Clinically significant differences among the environmental conditions and among the wire sizes were found. The introduction of saliva substitute provides a significant reduction in force values.

Dental Stress Analysis↗

Tetracycline-induced tooth changes. Part 5. Incidence in extracted first permanent molar teeth: a resurvey after four years.

First permanent molar teeth extracted during 1972 from patients aged 6 to 18 years attending the Royal Dental Hospital of Melbourne were sectioned sagittally and examined under ultraviolet light for fluorescent tetracycline deposits. The prevalence of fluorescent teeth increased with decreasing age to 84% in patients aged 10 to 11 years, then decreased in the younger children until only 67% were affected at six to seven years of age. Detailed analysis of the distribution of antibiotic in teeth demonstrated a marked decline in tetracycline experience since 1962-1964, i.e., fewer children received courses of the antibiotic, and confirms the trend demonstrated in a similar survey four years age. This trend is associated with a lower incidence and severity of crown discoloration and accompanying enamel hypoplasia in the youngest children examined.

Adolescent↗