PubMed Health⌕ Search

Biomedical subjects

K L Gera

Publications and source records attributed to K L Gera.

7 recordsLinked to original sources
PubMed1976

Hepatitis B-antigen subtypes in Australia as detected by radioimmunoassay.

The distribution of the major subtypes of hepatitis B surface antigen (HBsAg) was examined in asymptomatic white Australian blood donors and patients with liver disease to attempt ot assess the biological significance of these subtypes in this relatively homogeneous Caucasian population. Since previous studies have shown a very low prevalence of HBsAg in white Australians, a highly sensitive solid phase radioimmunoassay (RIA) was developed and used to subtype the antigen. A total of 46 samples of hepatitis B-antigen positive sera, obtained from 21 asymptomatic volunteer blood donors and 25 patients with liver disease, were tested. Sixteen of these 21 asymptomatic carriers were shown to have subtype "ad" whereas only seven of the patients with liver disease had this subtype. This difference was significant (P less than 0.005) and supports the view that subtype "ad" is associated with a carrier state and subtype "ay" with liver disease in Australia.

Acute Disease↗
PubMed1975

Solid phase radioimmunossay for serum ferritin.

A solid-phase "direct" radio immunoassay which is highly sensitive (detecting less than 0.5 ng/ml) and reproducible is described for the assay of ferritin in human serum. The method makes use of specific anti-ferritin antibody prepared using human liver ferritin; It has the advantage that tubes coated with specific antiserum may be stored for later use and lends itself readily to an automated washing procedure. The concentration of ferritin present in the serum of normal males in the population was 56,9 ng/ml (geometric mean) with a 95 percent confidence range of 18 to 180 ng/ml. In normal females the concentration was 34.0 ng/ml (geometric mean) with a 95 percent condidence range of 10 to 143 ng/ml.

Animals↗