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Biomedical subjects

K L Jahans

Publications and source records attributed to K L Jahans.

13 recordsLinked to original sources
PubMed2006

Camel tuberculosis--a case report.

An adult male dromedary bull was diagnosed with pulmonary tuberculosis (Tb). The dromedary was severely emaciated and died 2 months after the onset of the disease. It exhibited typical Tb lesions in both lungs and lung lymph nodes. A guinea pig inoculated with lung tissue from theTb camel died after 3 weeks from typical Tb. Mycobacteria were isolated from the dromedary's lung and lung lymph nodes and also from different organs of the guinea pig. The microorganism was identified as member of the antelope clade of the Mycobacterium tuberculosis complex.

Animals↗
PubMed1997

The characterisation of Brucella strains isolated from marine mammals.

Small Gram negative coccobacilli isolated from seals, porpoises, dolphins and from an otter road casualty were identified as Brucellae by their colonial and cell morphology, staining characteristics, biochemical activity, agglutination by monospecific antisera and susceptibility to lysis by Brucella specific bacteriophage. Their characterisation, including metabolic profiles, is described. These strains could not be assigned to recognised nomen species of the genus Brucella and it is suggested that they comprise a new nomen species to be called B. maris (sp. nov., type strain 2/94). It is further suggested the nomen species be subdivided into three biovars corresponding to their CO2 requirement, metabolic activity on galactose, dominant antigen and animal host.

Animals↗
PubMed1997

The differentiation of Brucella species by substrate specific tetrazolium reduction.

In the development of a tetrazolium reduction assay to replace substrate stimulated oxygen uptake for the identification of Brucella species, nine tetrazolium salts were evaluated. Only the more readily reduced compounds (MTT and INT) detected increased metabolic activity with the more fastidious, slow growing strains and with B. suis strains on L-arginine and DL-ornithine. The assay was optimised with MTT. MTT reduction profiles offered with the medium on which the cells were grown. Cells grown on TSA gave profiles more similar to the published respirometric results than those grown on SDA. The optimal substrate concentration was 0.84 g l-1 and prolonged (> 3 h) exposure to substrate was necessary before adding MTT. MTT concentration was not critical and the OD was proportional to the MTT concentration between 0.03 and > 0.5 g l-1. MTT reduction was linear for 60 min after its addition. The reaction was, therefore, stopped after 60 min by adding formaldehyde solution. The optimised assay was evaluated with 71 strains of Brucella, representing all the species and biovars of the genus. Each strain was assigned to its previously identified species and sub-groups were defined.

Amino Acids↗
PubMed1996

Isolation of Brucella species from cetaceans, seals and an otter.

Brucella organisms which differed from the recognised species of the genus, were isolated from nine seals, eight cetaceans and one otter. A method is described for the isolation of Brucella species from sea mammals and the first isolations of Brucella species are recorded from an Atlantic white-sided dolphin (Lagenorhynchus acutus), two striped dolphins (Stenella coeuleoalba), a hooded seal (Cystophora cristata), a grey seal (Halichoerus grypus) and a European otter (Lutra lutra). There were differences in the culture media required for the primary isolation of the organisms and in their dependency on carbon dioxide, Subcutaneous lesions, when present, always yielded a confluent growth. The organisms were isolated from seven of 14 spleen samples and also from the mammary glands, uterus, testes and blood and the mandibular, gastric, iliac, sub-lumbar and colorectal lymph nodes.

Animals↗
PubMed1992

Isolation of Brucella melitensis from aborting ruminants in Jordan.

Thirty-four isolates (16.5 per cent) of Brucella melitensis were cultured from 206 samples from aborted, still-born or weak full-term animals and vaginal swabs from aborted animals. Twenty-six of 31 isolates from sheep were B. melitensis biovar 3. Two isolates were biovar 1 and one of these was the vaccine strain Rev 1. The three remaining isolates were from vaginal swabs and were not biotyped since B. melitensis biovar 3 had been isolated from the aborted fetuses. One of two isolates from cattle and one isolate from a goat were biovar 1. The remaining cattle isolate was biovar 3.

Abortion, Veterinary↗
PubMed1990

Brucella biovars isolated from nomadic cattle in the southern Darfur Province of western Sudan.

The occurrence of brucellosis and the identification of biovars of Brucella species isolated from infected cattle were studied in nomadic, semi-nomadic and sedentary populations in the South Darfur Province of Western Sudan. Brucellosis was widespread in the area and in all management systems investigated. Forty-one strains of B. abortus were isolated and all were found to be biovar 6.

Animals↗
PubMed1990

The isolation of Brucella melitensis biovar 3 from a testicular hygroma of a ram in a nomadic flock of sheep and goats in western Sudan.

Brucella melitensis biovar 3 was isolated from a testicular hygroma in a ram from a nomadic flock of sheep and goats serologically positive for brucellosis and with a history of occasional abortions. The affected testis was enlarged, extensively damaged and contained fluid. The isolation of this biovar identifies the need for further investigations into the prevalence of brucellosis and the particular biovars involved in nomadic animals.

Animals↗
PubMed1990

Clinical manifestations of brucellosis in cattle of the southern Darfur Province, western Sudan.

Ninety-seven adult Zebu cattle with hygromas, 32 with arthritis and two showing long calving intervals were investigated for brucellosis. 92 per cent of hygromatous cattle, 62 per cent of arthritic cattle and both animals having long calving intervals were serologically positive and 65 per cent of milk samples were positive by the Milk Ring Test. Hygromas were found on the jaw, bursa, thigh, flank, hip, shoulder, neck and joints. Hygroma aspirates gave higher titres than sera in the serum agglutination test. IgG1, IgG2 and IgA were detected in all the aspirates and all but one contained IgM. Cattle with hygromas are a potential source of infection to other animals and a serious public health problem.

Animals↗
PubMed1987

Effect of infectious stunting syndrome on the immunocompetence of broilers.

Immune responses and liveweights of broilers affected with the stunting syndrome (group C) were compared with those of unaffected birds from the same premises (group B) and normal control broilers of the same genetic origin (group A). Measurements of cell-mediated immunity were made using the phytohaemagglutinin (PHA) intradermal test and those of humoral immunity by antibody response to sheep red blood cells. Weekly observations were made of birds aged from two to eight weeks. Throughout the investigation the weekly mean weight of affected birds was very significantly lighter (P less than 0.001) than that of unaffected and control birds. From the second to the sixth week, wing web PHA reactions in group C were very significantly less than those in groups B or A (P less than 0.001), and during the seventh and eighth week wattle PHA reactions were significantly less in group C than those in group B (P less than 0.02). No significant differences were detected between the three groups in humoral immunity; the complete, IgG and IgM, antibody levels being closely similar. The significance and possible practical applications of these findings are discussed.

Animals↗
PubMed1978

The isolation of an atypical Mycobacterium from three wood pigeons with tuberculosis-like lesions.

Cultural and biological tests were carried out on the livers and spleens of three wood pigeons. Acid-fast organisms were observed in histological sections and smears taken from tuberculosis-like lesions in the organs. Biological tests revealed that all the isolates sensitised the guinea pig to avian tuberculin. One was pathogenic for chickens while the remaining two were non-pathogenic. All were non-pathogenic for rabbits and domestic pigeons. The organisms could not be cultured on conventional media for Mycobacterium tuberculosis or media containing mycobactin. However they were readily maintained on 7H11 media. These results indicate a separate species of mycobacterium related to M avium.

Animals↗