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Biomedical subjects

K Leonhardt

Publications and source records attributed to K Leonhardt.

8 recordsLinked to original sources
PubMed2000

Instituting medical abortion services: changes in outcome and acceptability related to provider experience.

A prospective case series carried out at 34 Planned Parenthood sites studied the safety, efficacy, and acceptability of medical abortion with methotrexate and misoprostol in 1973 women. Women with pregnancies of 26 to 49 gestational days were given methotrexate followed by vaginal misoprostol. Eighty-one percent of women had documented complete medical abortions; abortion was not confirmed by examination in 6%, and 13% had documented suction curettage. The rate of suction curettage decreased with site experience, from 17% during the first 20 procedures to 10% at sites that had performed more than 50. Sites that had previous experience with either methotrexate or mifepristone medical abortion had a rate of 9% after they had performed at least 50 procedures. Exit interviews with 755 of the 902 women having abortions in the first year inquired about the level of comfort with the abortion and its overall acceptability. Women's satisfaction with the side effects did not directly correlate with site experience. Overall satisfaction with the abortion experience was related to whether the women had complete medical abortions or suction curettage.

Abortifacient Agents, Nonsteroidal↗
PubMed1994

Photosystem II genes isiA, psbDI and psbC in Anabaena sp. PCC 7120: cloning, sequencing and the transcriptional regulation in iron-stressed and iron-repleted cells.

Under conditions of iron deprivation cyanobacteria produce flavodoxin to replace ferredoxin as the terminal electron acceptor of photosynthesis. In unicellular cyanobacteria, the gene for flavodoxin is the second open reading frame in a dicistronic operon whose transcription is tightly regulated by iron. The first gene, isiA, produces a protein that is very similar to CP43, a chlorophyll-binding, antenna protein of the photosystem II reaction center. In the filamentous, heterocystous cyanobacterium Anabaena sp. PCC 7120, isiA and the gene for flavodoxin are located in separate operons with independent promoters. In this paper, we report on the sequence of isiA and show that it is found in a monocistronic operon that is transcriptionally regulated to be expressed under iron stress but does not produce detectable transcripts under conditions of iron repletion. We also report on the sequence, organization and expression of the gene that codes for CP43, psbC. In Anabaena sp. PCC 7120, psbC has a genetic organization similar to that of other cyanobacteria and higher plants; the 5' end of psbC overlaps the 3' end of psbDI. Transcriptional analysis of the psbDC operon showed that it is constitutively expressed in both iron-repleted and iron-stressed conditions; however, a new monocistronic transcript was detected that contains psbC and is preferentially expressed under iron stress conditions.

Amino Acid Sequence↗
PubMed1992

An iron stress operon involved in photosynthetic electron transport in the marine cyanobacterium Synechococcus sp. PCC 7002.

The iron-stress-induced genes isiA and isiB have been cloned and sequenced from the marine unicellular cyanobacterium Synechococcus sp. PCC 7002. These genes code for a photosystem II chlorophyll-binding protein and flavodoxin respectively. The genes form a dicistronic operon that is transcriptionally activated under iron-stress conditions to produce an abundant monocistronic message containing isiA and a much less abundant dicistronic message that also contains isiB. The arrangement of these genes, their transcriptional control and the relative abundance of the monocistronic and dicistronic messages produced under iron stress parallels the pattern shown by the freshwater cyanobacterium Synechococcus sp. PCC 7942. The genes for the corresponding proteins found under iron-replete conditions, CP-43 and ferredoxin, have also been cloned and sequenced. Northern blot analysis indicates that both of these genes are constitutively expressed under both iron-stress and iron-replete conditions.

Amino Acid Sequence↗
PubMed1990

[Vocational rehabilitation of psychiatrically handicapped people].

The article describes the working concepts of "Lankwitzer Werkstätten", a facility for vocational rehabilitation of persons with (chronic) mental illness. The working concept of this sheltered workshop is crafts- and service-oriented. Some 60 rehabilitees and sheltered workshop employees work in four small "shops". Work structures and everyday organization are somewhat unusual in that they follow general labour market conditions as closely as possible. In their working structures, the sheltered workplaces usually do not differ from workplaces in small crafts enterprises of the general labour market. As far as remuneration is concerned, the workshop is in a position to at least envisage levels of pay that will ensure independence from social assistance supplements if the facility's technical equipment can be kept current and work planning made more efficient. Within the limits of the legal possibilities, and making the most of them, both the diagnostic-prognostic benefits of the entry procedure in the work training process as well as the placement opportunities for rehabilitees in the general labour market are being utilized, with occasionally very successful outcomes.

Chronic Disease↗
PubMed1989

[Dysplasia epiphysealis hemimelica--a case report].

One case of dysplasia epiphysealis hemimelica is described. It is an uncommon disorder belonging to the group of osteochondromatoses. The disease occurs on young children and is localized mainly in the ankle and knee joints. The time in which the patient came to our hospital was to late to make a causal operation. It is the reason to describe this case of dysplasia epiphysealis hemimelica.

Adolescent↗
PubMed1987

6-Methylpurine, 6-methyl-9-beta-D-ribofuranosylpurine, and 6-hydroxymethyl-9-beta-D-ribofuranosylpurine as antiviral metabolites of Collybia maculata (Basidiomycetes).

6-Methylpurine, 6-methyl-9-beta-D-ribofuranosylpurine and 6-hydroxymethyl-9-beta-D-ribofuranosylpurine were isolated from mycelial cultures of Collybia maculata and their antifungal, cytotoxic and antiviral activities investigated. This is the first report on the natural occurrence of these compounds.

Adenosine Deaminase Inhibitors↗