[The study of the relationship between polymerized human serum albumin receptor and HBV replication].
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Adolescent↗
Biomedical subjects
K M Chia
Publications and source records attributed to K M Chia.
[Evaluation of serological exclusion methods in diagnosing non-A, non-B hepatitis].
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Diagnostic Errors↗
Clinical significance of enhanced sensitivity in detecting HBsAg by Biotin-Avidin ELISA.
The Biotin-Avidin System (BAS) was incorporated into enzyme-linked immunosorbent assay (ELISA) technique to increase the sensitivity of the standard ELISA for the detection of HBsAg. We compared the sensitivity of Biotin-Avidin ELISA (BA-ELISA) with monoclonal antibody reverse passive hemagglutination assay (McAB RPHA), standard ELISA and solid phase radioimmunoassay (SPRIA). BA-ELISA was most sensitive. Out of 276 healthy individuals who were HBsAg negative by polyclonal antibody RPHA (PcAB-RPHA), 10(3.60%) were HBsAg positive by the BA-ELISA. Out of 123 acute hepatitis patients who were HBsAg negative by PcAb-RPHA and anti-HBc positive by ELISA, 64(52.03%) were found to be HBsAg positive by BA-ELISA. This study indicates that enhanced sensitivity of BA-ELISA in detecting HBsAg significantly increases the correctness of diagnosis of HBV infection.
Avidin↗
[New development in the study of infectious hepatitis].
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Acute Disease↗
[New development in infectious hepatitis].
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Azathioprine↗