Biomedical subjects

K M Kamal

Publications and source records attributed to K M Kamal.

4 recordsLinked to original sources

PubMed2001

Microsurgical varicocelectomy in the era of assisted reproductive technology: influence of initial semen quality on pregnancy rates.

OBJECTIVE: To evaluate the natural history of infertile couples in whom the man underwent varicocelectomy. DESIGN: Retrospective study. SETTING: University infertility clinic. PATIENT(S): 159 infertile couples. INTERVENTION(S): Microsurgical varicocelectomy. MAIN OUTCOME MEASURE(S): Standard semen parameters and pregnancy rates (assisted and unassisted). RESULT(S): Postoperative sperm concentration and motility increased significantly compared to before the varicocelectomy (28.9 +/- 3.0 vs. 22.5 +/- 2.1 x 10(6)/mL and 33.9 +/- 1.9 vs. 22.1 +/- 1.3%, respectively). Overall, 48% (76/159) of the couples achieved a spontaneous pregnancy at the mean of 30 months' follow-up. An additional 10% (16/159) and 11% (18/159) of couples achieved a pregnancy following intrauterine insemination and in vitro fertilization/intracytoplasmic sperm injection, respectively. Significantly higher spontaneous pregnancy rates were observed in couples in whom the man's initial sperm concentration was greater than or equal to 5 million sperm per milliliter compared to those in whom the man's initial sperm concentration was less than 5 million sperm per milliliter (61% vs. 8%, respectively). CONCLUSION(S): Our data demonstrate that, in couples in whom the man undergoes varicocelectomy for treatment of varicocele, close to 70% achieve a pregnancy. The initial sperm concentration is predictive of unassisted pregnancy outcome in this population.

Adult↗

PubMed2001

Free thiols in human spermatozoa: correlation with sperm DNA integrity.

OBJECTIVES: To examine the levels of sperm protamine free sulfhydryl or thiol (SH) groups and DNA denaturation (DD) in semen from fertile and infertile men.Methods. Semen samples were obtained from consecutive nonazoospermic men presenting for infertility evaluation (n = 66) and fertile men presenting for vasectomy (n = 10). The standard semen parameters (sperm concentration, motility, and morphology), sperm DD, and SH content were monitored. Sperm DNA integrity was evaluated by flow cytometry analysis of acridine orange-treated spermatozoa and expressed as the percentage of spermatozoa demonstrating denatured DNA. RESULTS: The mean (+/- SE) levels of sperm SH groups and the percentage of spermatozoa with DD were significantly higher in the infertile men than in the fertile men (SH groups 1083 +/- 97 and 570 +/- 101 nmol/10(6) spermatozoa, respectively; and DD 21% +/- 1.7% and 8.9% +/- 1.9%, respectively). The levels of sperm SH groups and DD correlated positively (r = 0.53). Also, the levels of sperm SH groups and DD correlated negatively with the standard semen parameters. CONCLUSIONS: Our data demonstrate that sperm SH content correlates positively with sperm DD and that significantly higher levels of sperm DD and SH content are found in infertile men than are found in fertile men. These data suggest that the enhanced susceptibility of sperm DNA to denaturation in infertile compared with fertile men may be associated with incomplete oxidation of the sperm SH groups.

DNA↗

PubMed1997

Antiallergic potential of furosemide.

Recent reports have indicated the effectiveness of furosemide in inhibiting responses to inhaled allergen and in treating allergic conjunctivitis. In the present study furosemide was tested for its antiallergic potential using compound 48/80 induced paw edema and in vitro mast cell degranulation. Furosemide was found to significantly inhibit compound 48/80 paw edema and compound 48/80 induced histamine release. Furosemide was also found to inhibit histamine release during passive peritoneal anaphylaxis in rats. The results suggest that furosemide may be inhibiting the release of mediators of anaphylaxis from the mast cells.

Animals↗

PubMed1993

[Hysterosalpingography using iotrolan--a new contrast medium].

Iotrolan was investigated as a new contrast medium for hysterosalpingography. Fertility rates and side-effects were registered in a series of 108 consecutive patients. The results indicate a higher fertility rate after hysterosalpingography with iotrolan than after the use of other water-soluble contrast media but a lower fertility rate when compared to lipiodol. As for side-effects, the study indicates that the frequencies of low abdominal pain and vaginal bleeding after hysterosalpingography are equal for iotrolan and lipiodol but higher for other, water-soluble contrast media. A prospective, randomized study is recommended for the clarification of these aspects.

Abdominal Pain↗